*j>. V \ V .^ -> .J " fV^ > I > :*& ./::.' -*■ r- Issued February 10, 1913. U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 105. B. T. GALLOWAY, Chief of Bureau. EXPERIMENTS IN THE CONTROL OF GRAPE ANTHRACNOSE. BY LON A. HAWKINS, Scientific Assistant, Fruit-Disease Investigations. 05010° 13 WASHINGTON : GOVERNMENT PRINTING OFFICE : 1918 • rf>3 BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A. Taylor. Editor, J. E. Rockwell. Chief Clerk, James E. Jones. [Cir. 105] 2 B. P. I— 790. EXPERIMENTS IN THE CONTROL OF GRAPE ANTHRACNOSE. INTRODUCTION. Anthracnose of the grape, or bird's-eye rot, caused by the fungus Sphaceloma am/pelinum De Bary, is a disease of considerable impor- tance on certain varieties of grapes grown in the eastern United States. While the disease does not seem to attack the Concord, which is the most extensively grown of American varieties of grapes, Vergennes, Salem, Diamond (Moore's Diamond), Norton (Norton's Virginia), Missouri Riesling, Diogenes, Champion, and other varieties of both wine and table grapes are very susceptible to anthracnose. In serious outbreaks of the disease the fruit in a vineyard of one of these susceptible varieties may be almost entirely destroyed and the vines seriously damaged. Certain exceptionally fine varieties of wine and table grapes can not be grown successfully in some localities because of unusual susceptibility to it. Anthracnose affects practically all the green parts of the plant. On the green shoots it causes cankers (PL I, fig. 3), which first appear as small, light-brown, slightly sunken spots. These spots enlarge, sink deeper, and become darker in color as the disease progresses. If the canker is near the base of a shoot, the whole shoot may be killed. If not killed directly by the disease, the shoot may be so weakened as to be easily broken. The fungus causes cankers on the petioles and ribs of the leaves (PI. I, fig. 2), on the stems of bunches of grapes, and on the tendrils. On the grape berries it first appears as light-brown spots, which rapidly become darker and are soon surrounded by a circular bright-red zone, which, as seen against the green background of the grape, presents the very striking appearance that gives the disease the name of "bird's-eye rot." The berries in later stages of the disease shrivel and dry up (PI. I, fig. 1). The fungus is also reported to be the cause of a leaf-spot. The writer has, however, never observed any case of leaf-spot on grape leaves that could be attributed to it. The fungus is supposed to live over the winter in the old wood and in mummied grapes. It repro- duces itself by means of minute, oblong, colorless spores, which infect the young, green parts of the plant. The disease is well known in Europe and, according to reports, may be controlled by proper treatment. Very little has been done in America, however, toward working out the best method for preventing the disease, and the recommendations for its treatment in this country are usually based [Cir. 105] 3 EXPERIMENTS IN THE CONTROL OE GRAPE ANTHRACNOSE. on its treatment abroad. As the climatic conditions of American and European vineyards differ, as well as the varieties of grapes usually grown in them, it was thought that it might be necessary to modify the treatment of the disease as practiced in Europe, in order to adapt it to American vineyards. The experiments described in this circular were accordingly taken up by the writer, under the direc- tion of Dr. C. L. Shear, Pathologist, of this Bureau, to see whether the disease could be controlled in this country by the use of a solution of iron sulphate and sulphuric acid in water, which is employed extensively in Europe. It was also considered worth while to experiment with applications of other fungicides to the dormant vines and to try the effect of summer sprays in comparison with the iron-sulphate and sulphuric-acid treatment mentioned. EXPERIMENTATION. The experiments which are here reported were conducted at Lawton, Mich., in a vineyard of Champion grapes belonging to Mr. John Robinson. In this vineyard in 1909 about two-thirds of the fruit had been destroyed and the young wood seriously injured by anthracnose. The vines selected for experimentation were a block of about 600 where the disease had been the most severe. This block was divided into nine plats, seven of which were to be treated and two to remain untreated as controls. The order in which the treated plats and controls w r ere arranged is shown in Table I. Table I.- -Treatment (riven plats of grapevines at Lawton, Mich., in 1910, 1911, and 1912 and the results obtained in 1911 and 1912. No. of plat. Treatment in 1910. Treatment in 1911. Diseased fruit in 1911. Treatment in 1912. Diseased fruit in 1912. 1 (When dormant.) Iron- sulphate mixture No. 1. (When dormant.) Iron- sulphate mixture No. 2. Control, untreated (When donnant.) Ten per cent solution of sul- phuric acid. (When dormant.) Con- centrated lime-sulphur solution (1-10). Same as in 1910 Per cent. Same as in 1910 Per cent. 2 Same as in 1910. . Same as in 1910 3 4 Control, untreated 4 per cent solution of sul- phuric acid. Same as in 1910 32.6 Control, untreated Same as in 1911 10 5 Same as in 1910 ....... Control, untreated Self-boiled lime-sulphur solution (8-8-50); four applications. 4-3-50 Bordeaux mix- ture; four applications. 4-3-50 Bordeaux mix- ture with two pounds of rosin-fishoil soap to 50 gallons of mixture; four applications. 12.2 Sprayed 5 times with 4-3-50 Bordeaux mixture. Two pounds of rosin-fish- oil soap were added in the last two appli- cations. do 7 8 ..do :. 'i 24.47 2.04 Rainfall by months. 1907 Inches. 0.56 1.72 1.70 4.50 2.81 .31 2.65 . 39 .64 2. 98 6. 78 .64 25.68 2.14 1908 Inches. 0.47 2.61 1.27 2.39 5.44 .88 1.37 3. 79 2. 5(1 1.62 2.87 1.50 26.80 2.23 1909 Inches. 0.00 .41 1.12 .85 1.72 .72 2.86 1.75 .29 1.20 .27 1.95 13.14 1.09 1910 Inches. I. lb .68 .21 4.4H 1.77 2. 24 1.12 .45 Jin 3.7(1 L.30 1.74 20.H2 1.67 1911 Inches. 0.16 1.73 3.34 23.41 1.41' .OS 1.14 2. lfi 1.24 3.09 2.13 1.53 21.42 1.64 1912 Inches. 0. 31 6.21 2.30 2.00 1.(4 3.42 .IIS 1 Average for the years 1891-1911 from records of the United States Weather Bureau. Rainfall from 190V to 1912 from records at the San Antonio Experiment Farm. -> Record for April, 1911, from the United States Weather Bureau service at San Antonio. The rainfall for the years 1909 to 1911 w^as considerably below normal, while that of 1909 was the lowest thus far recorded. Con- sequently the crop yields reported in these experiments were rather lower than may be expected under normal conditions. The year 1908, as indicated by the crop yields, w r as perhaps more favorable than normal, although the total rainfall for the year does not indi- cate it. The heavy rains in November of the preceding year w r ere apparently stored in the soil and used during the season of 190S. FACTORS INFLUENCING CROP YIELDS. The yields of forage crops reported in the following pages are extremely variable and often are not significant. They are pub- lished as a matter of record, but the interpretation of the experi- ments should be based on the printed conclusions and recommenda- tions rather than on the figures alone. It is not always clear which way the crop or the treatment of the land the previous season affected the subsequent crop yields. Usually, fallowing land for several months results in larger yields of the following crop, but this is not always the case. The kind of crop grown the previous year also affects the yields obtained. For instance, the small grains, such as wheat or oats, are harvested in May or early in June, corn in July, while cotton and sorghum occupy the land until late in October and use any available soil moisture until harvested. A crop planted the following March or April on land that had been devoted to cotton or sorghum is ordinarily at a disadvantage wdien compared with a crop on land that had been in oats or corn the previous year. It lias seemed advisable to publish the crop yields even at the risk of some misinterpretation, but in each case it is urged that for pur- [Cir. 106] FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. 7 poses of practical application the notes and comments concerning each crop or experiment be given the greater emphasis and that any broad generalizations from the figures in the tables be avoided. THE SOIL ON WHICH THE EXPERIMENTS WERE CONDUCTED. The San Antonio Experiment Farm is located in the southern extension of what is called the "Black Prairie region," known geologically as the Upper Cretaceous formation, and is representative of a large portion of central Texas extending from the Oklahoma line to the Rio Grande. The surface soil, which is a black, clayey loam, is capable of absorbing a high percentage of moisture, but tends to puddle and bake. It is very sticky when moist, and all tillage opera- tions are difficult because the plows and cultivators do not scour. The subsoil is of about the same mechanical composition as the surface soil, but it often contains more lime, which occurs as gravelly concretions. The subsoil is also much lighter colored, probably due in part to the lack of organic matter as well as to the larger proportion of lime. When supplied with plenty of water, the soil is usually very productive and has proved well adapted to growing many of the ordinary forage crops. CROPS ORDINARILY GROWN FOR FORAGE. The crops that are generally grown in the San Antonio region for forage are sorghum, oats, Johnson grass, millet, cowpeas, and corn, while alfalfa is grown to a limited extent where irrigation water is available. Of the crops mentioned, sorghum, oats, and Johnson grass are the most common, and they rank in the order named in popularity and value. The native wild grasses are used only for pasture. Most farmers grow only sorghum for forage, and conse- quently it has been deemed advisable to test other forage crops with a view to suggesting some practical diversification as a basis for systematic crop rotation. The native cactus, or Opuntia, is being used as a forage crop and has proved to be a valuable addition to the list, especially during unfavorable seasons. 1 SORGHUM. Experiments designed to determine the most prolific varieties, the best methods of planting, the proper rate of seeding, and the best date of planting sorghum have been carried on at the San Antonio Experiment Farm during the past five years. i For information concerning this crop, consult the following bulletins: ■Griffiths, David. The Prickly Pear as a Farm Crop. Bulletin 121, Bureau of Plant Industry, U. S. Dept. of Agriculture. 1908. The "Spineless" Prickly Pears. Bulletin 140. Bureau of Plant Industry, P. S. Dept. of Agri- culture. 1909. The Thornlcss Prickly Pears. Farmers' Bulletin 483, P. S. Dept. of Agriculture. Pill'. [Cir. 106] 8 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. Sorghum may be planted any time from March 1 to August 1 with the expectation of securing a crop, although as a rule the earlier the planting the larger the yield. When planted early in March, from two to four cuttings can be obtained from the earlier maturing varie- ties, such as Red Amber, while from the later maturing varieties, such as Sumac, two or three cuttings can be obtained under favor- able conditions. The varietal tests in 1908 indicated that Sumac and Red Amber were the best varieties for this region; hence, all later tests were carried on with these two varieties only. Table II gives the results of a test of varieties in 1908, which was an unusually favorable year. It has been observed that an unfavorable season often magnifies the differences in favor of the better varieties Table II. — Yield of sorghum varieties grown in one-tenth acre plats at (he San Antonio Experiment Farm in 1908. S. P.I. No. Variety. Date planted. Number of cut- tings. Yield per acre. 17554 Sumac Mar. 30 ..do 2 3 2 2 3 2 2 Tons. 12.29 17548 Red Amber 10.17 17556 Orange ... Mar. 18 ...do 7.12 17539 Planter's Friend 6.00 20799 Minnesota Amber ...do 5.38 17569 Kafir ...do 4.83 18684 Dwarf milo ...do 4.38 The sorghum was planted in rows 4 feet apart and the plats were one-tenth of an acre in size. As will be seen from Table II, the Sumac is the heaviest yielder of the varieties tested, but the hay is somewhat coarser than that from the other varieties. On the whole, the Sumac is deservedly the most popular variety grown. (PI. I, fig. 1.) In 1910 a comparative test of the Red Amber and Sumac sorghums was carried out under irrigation. Table III gives the results of this test. Table III. — Comparative test of the Red Amber and Sumac sorghum* grown under irriga- tion at the San Antonio experiment farm in 1910. Triplicate plantings. Red Amber sor- ghum. Sumac sorghum. Number of < 'in lings. Yield per acre. Number of cuttings. Yield per acre. 3 3 3 Average. Tons. 14.41 12.45 11.62 2 2 2 Tons. 14.75 14.00 12.60 12. 83 13. 78 [Cir. 106] Cir. 106, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate I. Fig. 1.— Sorghum in Shock, Showing the Method of Harvesting when a Heavy Crop is Grown. As Much as 13 Tons Per Acre of the Sumac Variety has been Grown without Irrigation. (Photographed June 8, 1908.) Fig. 2. -Broadcasted Sorghum on Field B5-6. There was Practically no Hay Yield from this Plat. (Photographed July 27, 1910.) Cir, 106, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate Fig. 1. -Drilled Sorghum on Field B5-14. The Hay Yield from this Plat was 3.76 Tons Per Acre. (Photographed July 27, 1910.) Fig. 2.— A Plat of the Appler Rustproof Oats. Probably the Best Variety for the San Antonio Section. (Photographed May 17, 1910.) FOKAdE-CROP EXPERIMENTS AT SAX ANTONIO. 9 It will bo observed that the average yield from the three plats of the Sumac variety was nearly a ton larger than from the three plats of the Red Amber. Another point in favor of the Sumac variety is that there were only two cuttings of the Sumac, while there were three of the Red Amber, and it costs more to harvest tliree crops than two. METHODS OF PLANTING. Two methods of planting are practiced by farmers, namely, plant- ing in rows far enough apart so that cultivation is possible, and planting closer, either in drills or by broadcasting. Practically no sorghum is grown under irrigation in cultivated rows, but in recent years an increasingly large area is being planted on dry land in culti- vated rows. When sorghum is planted in rows and cultivated, the crop may have to be harvested with a grain harvester or by hand if there 1 is a heavy growth, but if the rows are not more than 2 feet apart or the growth is not too heavy it may be cut with a mowing machine and handled in the ordinary manner. Even with the rows 4 feet apart the sorghum at the field station the past four years has been harvested in the same way as broadcasted sorghums. Drilled compared with cultivated rows. —The yields from sorghum grown without irrigation have been larger when planted in cultivated rows than when the crop was sown broadcast or in 8-inch drills. Unfortunately, this comparison was not made in 1908, which was a very favorable season. Table IV gives the yields in this experiment for the years 1907 to 1911. Table IV. — Yields per acre of Sumac sorghum planted in cultivated rows 4 feet apart and in 8-inch drills. 1907 1908 1909 1910 1911 Aver- Method. Num berof plats. Aver- age yield. Num- ber of plats. Aver- age yield. Num- Aver- ber of age plats, yield. Num- ber of plats. Aver- age yield. Num- ber of plats. Aver- age yield. age for four years. Rows 4 feet apart 5 2 Tons. 3.20 1.68 4 Tons. 13.02 4 3 Tons. 1.68 1.07 4 3 Tons. 3.06 .74 4 3 Tons. 3.44 4.36 Tons. 2.84 1.96 8-inch drills 1 There were no plats of Sumac sorghum close-planted in 1908. The great difference in yield between the cultivated plats and those in 8-inch drills in 1910, the comparatively slight difference hi 1909, and the difference in favor of the 8-inch drills in 1911 are due to the seasonal rainfall. As will be noticed by reference to the rain- fall table (Table I), the year 1908 was a very favorable one and much moisture that collected in the soil was carried over to 1909, while 1909 was exceedingly dry, and 1910 was more favorable dur- 69799°— Cir. 106—13 2 10 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. ing the early part of the season. The cultivation of the sorghum in 4-foot rows conserved a large part of the rainfall for the use of the crop, while the plats in 8-inch drills were dependent entirely upon the season's rain, as there was very little moisture carried over from 1909 and the rain that did fall was lost very quickly by evaporation. Up to about May 1 the spring of 1911 was unusually favorable, the rains coming so frequently that considerable periods passed when it was impossible to cultivate. Thus, before May 1 cultivation did not affect the yields as much as it would otherwise have done and the shortage of rain in June prevented a second cutting, enabling the drilled plat to outyield the cultivated plat. When a series of years is considered, it is safe to conclude that for unirrigated land planting in rows, so that cultivation is possible, will increase the yields enough to justify this method of planting. (PI. I, fig. 2, and PI. II, fig. 1.) The effect of the two systems of cultivation on the succeeding crops is also important. In the rotation experiments in 1911 there was a slight difference in the yield of corn and a very noticeable difference in the cotton yield in favor of the land on which sorghum had been grown in cultivated rows the previous year. Under irrigation the advantage was still with the cultivated rows. In rows 3 feet apart the yield was 14.41 tons per aero, while in 8-inch drills the yield was 12.6 tons. The difference in yield is, however, comparatively slight; and when one considers the expense of cultivating the rows, the quality of the forage, and the greater ease of handling the broad- casted sorghum it is doubtful whether it is profitable under irrigation to plant in rows and cultivate. Width of rows. — A test was made in one-ten th-acre plats of sorghum planted in rows, 2, 3, and 4 feet apart. Table V shows the yields from these plats. Table V. — Yields of Sumac son/hum planted in rows 2, ■ >. and 4 feet apart. Distance between rows. Yield per acre. 1910 1911 Average. 2 feet . . Tons. 5.07 5.90 5.00 Tons. 3.30 2.82 3.30 Tons. 4.19 3 feet . . 4.36 4 feet . 4.15 The plats of 1910 were planted in a field summer-fallowed in 1909, and consequently the yields are not comparable with those given in Table IY. The land on which the experiment was conducted in 1911 was cropped the previous year. In both years the quality of the forage was best on the plat with the rows 2 feet apart, with the forage from the rows 3 feet feet apart second in quality. While the differ- ences are not great, yet the quality of the forage was so much better rr-ir ififii FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. 11 from the closer plantings and cultivation with the ordinary farm machinery so much easier that undoubtedly a closer planting than 4 feet is advisable. Bate of seeding in rows. — In 1910 a test of Sumac sorghum in rows 4 feet apart, with the plants 1, 2, 4, and 6 inches apart in the row, was attempted. Owing to poor germination there was not much dilFerence in the resulting stands of the different plats, so that the results were of no comparative value. Table VI shows the yields of this experiment as carried out in 1911, the rows being 4 feet apart as before. . The figures are based on averages of actual counts of plants at the end of the growing season. Table VI. — Yields of Sumac sort/hum with the plants different distances apart in the row. Distance between plants. Yield per acre. Distance between plants. Yield per acre. 2 inches Tons. 3.30 2.70 7 inches Tons. 2 05 •1 inches. . 8 inches 1 97 These results are definite and conclusive, as they show very clearly the effect of reducing the thickness of the stand. There has been some discussion relative to the effect of thick seeding on the yields in cultivated rows, and the foregoing results bear directly on this question. Aside from the increased yields, the quality of the forage is so much better in the thicker secdings that if the yield were some- what less where thicker seeding is resorted to this method would still be advisable. Bate of seeding test in 8-inch drills. — There appears to be a wide difference of opinion in this locality regarding the proper rate to seed sorghum broadcasted or in close-drilled rows. To throw some light on the subject, the following test was made. On April 29, 1912, seven plats of one-tenth acre each were seeded to Sumac sorghum in 8-inch drills, the rate of seeding varying from 25 pounds to 174 pounds per acre. Table VII presents the results of the experiment. Table VII. — Rate-of -seeding test of Sumac sorghum in 84nch drills. No. of plat. Seed per acre. Yield per acre. 1 Pounds. 20 37 52 88 121 153 174 Tons. 4 54 2 4 97 3 5 12 4 5 27 5 5 nl 6 4 r >"> 7 4 'JO [Cir. 1061 12 FORAGE-CEOP EXPERIMENTS AT SAN ANTONIO. This season the best results were obtained from sowing 88 pounds to the acre, with 52 pounds and 121 pounds second and third, re- spectively. With these results as a basis, to seed a much greater or less amount does not appear to be advisable. The quality of the hay on plat 4 was fully as good as on the plats with a thicker seeding, and was naturally much better than on plats 1 and 2. SORGHUM-LEGUME MIXTURES. In 1008 a series of experiments was begun to test the growing of legumes intermixed with sorghums for forage. Considering the extent to which sorghum is grown and the way it reduces the pro- ductivity of the land on which it is grown continuously, there seems to be need of some practical way to lessen the injury to the soil, as well as to produce a better balanced ration. This work has a direct bearing on the production of a better quality, as well as on the main- tenance of the quantity of forage produced. METHODS OF PLANTING. Different varieties of cowpeas and other legumes and several different methods of planting were tested in 1909 on a small scale, to determine as far as possible from one year's work the best varie- t ies to grow and the best methods for growing them. The sorghum varieties were the Sumac and the Red Amber and the legume varieties the Iron, Whippoorwill, and Unknown cowpeas and a variety ol kulthi (Dolichos Ujlorus). Two methods of planting w-ere adopted in 1909, the row method and the band method. The band method consisted of planting two rows of sorghum 2 feet apart, with one row of the legume between, the bands being planted 3 feet apart and the intervening space kept cultivated. In the row method the sorghum and legume seeds were plant ed in the same row, the rows being 4.1 feet apart and the ground kept cultivated between the rows. The band method was not as successful as the row method, apparently because the legumes did not receive the benefit of cultivation. Of the legumes used in the mixtures, the cowpeas did the best, the Unknown cowpeas being on the whole slightly better than any of the other varieties. Table VIII gives the yields of the mixtures. There was one-thirtieth of an acre hi the plats planted by the band method and one-fortieth of an acre in those planted by the row method. The land was summer-fallowed the preceding year and was a Johnson-grass meadow previously. [Cir. 106] FORAGE-CROP EXPUEIMENTS AT SAN ANTONIO. 13 Table VIII. — Comparison of the row and band methods in 1909, on land summer- fallowed the preceding near. Mixture. Sumac sorghum Iron cowpeas Sumac sorghum Unknown cowpeas Sumac sorghum Whippoorwill cowpeas. - Sumac sorghum Knlihi i Dolichos biflorus) Average Yield per acre. Table IX. — Comparison of the roiv and band methods in 1910 on land cropped to son/hum the preceding year. Mixture. Yield per acre. Row method. Band method. Sumac sorghum Unknown cowpeas Sumac sorghum Brabham cowpeas. ...".. Sumac sorghum Kulthi ( Dolichos biflorus I Pearl millet Kulthi (Dolichos biflorus) Average Tons. 1.76 1.18 .70 1.17 1.21 1.21 Of the cowpeas planted by the band method fully 50 per cent died before reaching maturity, while the legumes planted by the row method made a good growth, although the sorghum was too thick, not allowing the legumes to develop as well as they probably would have done had the sorghum plants been farther apart. The band method, it will be noted, outyielded the row method in 1909, but this was due to the predominance of the sorghum and is no indication of the comparative success of the two methods, as the forage from the row method contained a much greater percentage of cowpeas. The total yield from the two methods of planting was the same in 1910, but the value of the forage from the row method was much higher, owing probably to the fact that the cowpeas had a better chance to develop than when the band method was used. A test of mixtures seeded in 8-inch drills was made under irrigation but was entirely too thick, so that the legumes were crowded out. The yields were about the same as would have been expected had the sorghums been planted alone. It is very doubtful whether it is advis- [Cir. me,] 14 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. able to plant the sorghum-legume mixtures under irrigation in this manner, and it is certainly not advisable to do so without irrigation. Even with a thin rate of seeding the sorghum plants stool profusely and have a tendency to crowd out the shorter leguminous plants. So far, none of the tests with this method of planting have been satis- factory. On a field which was fallow in 1909 and planted in Johnson grass previously, a rate-of-seeding test for sorghum-legume mixtures was made in 1910, to determine as far as possible in one year's test the best proportion of each seed for the mixture. The row method of planting was adopted and the rows were 3^ feet apart, the plats being one-tenth of an acre in size. Sumac sorghum and Unknown cowpeas were the varieties used in this test. Table X shows the results of this test. Table X. — Yields of rate-of-seeding test of sorghum-cowpea mixtures, planted by the row method in 1910. Mixture. Sorghum Cowpea . Sorghum Cowpea . Sorghum Cowpea. Sorghum Cowpea . Stand, plants in 50 feet. 95 90 74 100 70 105 30 100 Number ol- rul tings. } Yield per acre. Tons. 4,: 5. 15 4.02 2.35 With all points considered, the second plat showed the best rate of seeding, although a test of one year is not conclusive. (Fig. 1.) In the first plat the sorghum appeared to be a little too thick and crowded the cowpeas, reducing the quantity of forage from them. In the case of the last two plats the sorghum was entirely too thin, mak- ing it rather coarse, but the cowpeas made an excellent growth. While the combination of sorghum and cowpeas is recommended, especially for the irrigation farmer, yet it should be borne in mind that extreme care must be exercised to secure the proper mixture. If the sorghum is too thick, the cowpeas will not properly develop and the forage will be little better than if the sorghum were planted alone. On the other hand, if there is too great a proportion of cowpeas the yield will be materially reduced and will prove a disappointment to the grower. Sumac sorghum is undoubtedly the best variety to use when these mixtures are planted, owing to its leafy character and slow growth at [Cir. 106] FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. 15 the beginning of the growing season. Early-maturing sorts like the Red Amber crowd out the cowpeas. The yields from the plats of sorghum indicate that fully as much fodder can be obtained in the mixture as by seeding alone; therefore, the advantage derived from the admixture of the cowpea hay, if not obtained at too great an expense in seeding and cultivation, is well worth while. Fig. 1.— Sumac sorghum and Unknown cowpeas planted in the same row, showing the excellent growth made by both the cowpeas and the sorghum. There are about three plants of the sorghum to four of the cowpeas. (Photographed July 27, 1910.) SMALL GRAINS AS FORAGE CROPS. 1 While in the immediate vicinity of San Antonio the grain yields from such cereals as oats, barley, wheat, and rye are low, yet oats, and rye to a lesser extent, are quite extensively grown for forage. Oats are also very useful for whiter pasturage, and are generally a ' The growing of cereals for grain production only at low altitudes in the vicinity of San Antonio is not a profitable venture. The principal cause of low yields is the susceptibility of grains to rust. In higher alti- tudes cereals are grown to a considerable extent. In the high land near Kerrville, and even no farther distant than the town of Boerne (30 miles), satisfactory yields have been realized from wheat and>oats. [Cir. 106] 16 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. profitable crop. The oats are ordinarily seeded in October or Novem- ber and are not pastured until the plants are well established, which is some time in December, depending on the weather conditions. If a hay crop is to be secured, the stock is taken off as soon as the spring growth starts. Little is known as to the extent of the damage done to the crop by grazing, but farmers believe that the whiter pasturage alone hi a year of normal rainfall is worth all it costs. Table XI shows the yields of oats grown during the years 1908 to 1911. In 1910 three local varieties were obtained to compare with four varieties introduced by the United States Department of Agri- culture. These varieties were the Texas Red Rustproof, Hastings' Hundred Bushel, and the Winter Turf. The plats were one-fifth of an acre in size, and the land had been summer-fallowed the preceding year in each case. Table XI. Yields of oats hay at the San Antonio Experiment Farm, in 1908-1911, inclusive. Variety. Yield of hay (for single plats, except as indicated). 1908 1909 1910 1911 Average. Appier Rustproof Pounds. 4,720 4,170 Pounds. 2,399 1 1,990 Pounds. 2 1,474 2 1,146 1,260 800 3 2, 000 3 1,780 3 940 Pounds. 1,855 Failed. 2,120 2,130 1,730 Pounds. 2 624 Culberson Winter 1 826 Texas Red Rustproof 1 690 Hastings' Hundred Bushel 1 465 W inter Turf 1 865 l 780 Snoma 940 1 Averages of four plats. 2 Averages of five plats. 3 The actual weight on these plats was less than the figures indicate, as the hay was not thoroughly dry when weighed. From the tests made thus far the variety of oats known as the Appier Rustproof has proved to be the best for this locality, although the 191.1 test apparently does not indicate this superiority. The low yield of the Appier Rustproof oats hi 1911 was probably due to the following causes: The four plats, which were planted with different rates of seeding, were located north of four plats of Culberson winter oats, which were so severely affected with rust that they were not worth cutting. As the prevailing winds are from the southeast, the rust spores were undoubtedly carried from the Culberson Whiter oats to the Appier Rustproof oats, causing a much more severe infestation than would otherwise have been the case. A plat of Appier Rust- proof oats on the south side gave a hay yield of 2,260 pounds, and the average yield of this variety in the rotation plats was 2,437 pounds per acre. (PI. II, fig. 2.) [Cir. 100] FORAGE-CROP EXPERIMENTS AT SAN ANTON lo. 17 Since the Appier Rustproof oats proved to be the best, in the fall of 1909, 39 half-bushel lots of these oats were sent to different farmers in the San Antonio region. Later a circular letter was sent out, request- ing a report on the results obtained with the variety, and its value as compared with the variety ordinarily grown. Twenty-six replies were received, of which 11 were favorable, 3 , unfavorable, and the remaining 12 equally divided — 6 did not think the test was satisfac- tory, and found little difference between this variety and that com- monly grown. In 1909 a ratc-of -seeding test was made to determine the rate that would give the highest yield. The varieties of oats planted were Appier Rustproof and Culberson Winter. Table XII presents the results of the test. Table XII. — Yields <;/' Appier Rustproof and Culberson Winter oats in n rale-qf- seeding test, 1909. Variety. Kale of set 'ding. Approxi- mate num- ber of seeds per acre. Yield per acre. Si raw. Grain Culberson Winter I ><> Do .1)0 A ppler Kusl proof Do.- Do Do Pounds. 59 43 28 23 59 43 30 24 2. 124, 000 1,550,000 1,008,000 828, 000 950,000 700,000 480. 000 384,000 Pounds. 1,750 1,835 2,065 2,310 2,600 2,500 2,225 2,200 Bushels. 6. 87 7.85 11.76 13. 05 19. 06 is. (HI 16. 13 13.44 Apparently conflicting results were obtained hi this test. With the Culberson Winter oats the lightest seeding nearly doubled the grain yield and gave an increased yield of 560 pounds of hay. With the Appier Rustproof oats, however, the heaviest seeding yielded 5.62 bushels of gram and 400 pounds of hay more than the lightest. By taking a count of the oats it was found that there were about two and one-half times as many seeds of the Culberson Winter oats as there were of the Appier Rustproof oats in a single pound, so that in the heaviest seeding of the latter the number of seeds per acre was only slightly greater than the lightest seeding of the former. Although it is to be expected that the seasonal variations will change the results somewhat, yet the results from the season of 1909 indicate that 2 bushels of seed per acre is about the right quantity to sow. This test shows conclusively that the same rates of seeding of two varieties are not necessarily comparable and that the size and weight of the seed are important factors to be considered. For further information as to the best rate of seeding this experi- ment was repeated in 1910. Table XIII shows the yields for the year. [Cir. 106] 18 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. Table XIII. — Yields in a rate-of-seeding test of Appier Rustproof and Culberson Winter oats, in 1910. Variety. Rate of seeding. Yield per acre. Straw. Grain. Appier Rustproof. . Pounds. 71 59 45 30 37 28 23 14 Pounds. 1,890 1,225 1,609 1,320 1,000 1,200 1,280 1,050 Bushels. 21.5 Do 14.0 Do 16.4 Do 15.2 Culberson Winter . 8.6 Do.. 7.2 Do 9.1 Do 6.6 With Appier Rustproof oats the heaviest seeding gave the highest yield, but with Culberson Whiter oats there was so little difference that the deviation could be attributed to variation in the soil. OTHER SMALL GRAINS THAT MAY BE USED AS FORAGE CROPS. Other small grains which have been tested for use as forage crops are wheat, barley, emmer, and rye. Table XIV shows the yields from these cereals. These plats were one-tenth of an acre in size and the land was summer-fallowed the previous season. Table XIV. — Hay yields from various cereals tested for forage. ( Viral. Hay yield. 1910 1911 Abruzzes rve Pounds. 2,480 1,100 1,740 '800 Pounds. 3,380 Rieti wheat Black winter emmer Barley 2 1,951 1 Average yield from six varieties. 2 Average yield from eighl varieties. With the exception of rye, none of the cereals mentioned in Table XIV gives indications of being of any great value for this section, from a forage standpoint. With the exception of rye, all those listed are very susceptible to rust. While the variety of rye grown appears to be somew r hat better than oats from the standpoint of yield, yet the value of oat hay is somewhat higher. Rye comes second on the list of cereals for hay and is recommended next to oats. OTHER FORAGE CROPS. JAPANESE SUGAR CANE. A one-tenth-acre plat of Japanese sugar cane was planted at the field station on March 22, 1910. This is a forage crop of value in Florida [Cir. 106] Cir. 106, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate III. Fig. 1.— Japanese Sugar Cane Grown Under Irrigation. A Promising Crop for THE IRRIGATIONIST. (Photographed November 1, 1910.) Fig. 2.- Unknown Cowpeas Planted in Rows 3 Feet Apart. Cowpeas are a Crop that should be More Widely Grown in the San Antonio Section. (Photographed July 27, 1910.) Cir. 106, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate IV. Fig. 1.— Canada Field Peas Grown without Irrigation. These Peas Gave a Yield of 1.9 Tons per Acre. (Photographed May 19, 1911.) Fig. 2.— Guinea Grass Grown Under Irrigation. This Grass has Possibilities as a Forage Crop when Irrigated. fPhotoeraiiln'il November 1, I'.tlO.I FORAGE-CEOP EXPERIMENTS AT SAN ANTONIO. 19 and the Gulf coast region. The yield from the plat, planted under irrigation, was 13.5 tons per acre in 1910, and 11.95 tons per acre in 191 1 . (PL III, fig. 1 .) To obtain the best results late fall planting is recommended. This cane promises to be a valuable addition to the list of forage crops that can be grown under irrigation in this section, as it makes a large yield of very palatable fodder. In the plats tested the canes were planted in furrows about 6 inches deep and 6 feet apart. Ordinary cultivation was given the crop. Mr. John M. Scott, in a recent bulletin, 1 says that the number of whole canes required to plant an acre is about 3,000. He suggests that the rows be made about 8 feet apart and that the canes be cut in pieces having three or four eyes to each piece and dropped in a double line. There is some reason for believing that a closer planting than 8 feet may be advisable in Texas as the 1 canes would then be less coarse. ALFALFA. A number of tests have been made with different varieties of alfalfa and with different methods of seeding this crop. None of the results so far obtained have been sufficiently satisfactory to warrant alfalfa being grown on a commercial scale without irrigation. The principal reason for this difficulty seems to be that in the immediate locality of San Antonio it is not possible to store enough moisture in the soil to carry the plants over the periods of drought that occur in the summer. If there were no loss by surface run-off and evaporation, both of which are important factors in the section mentioned, the crop could prob- ably be grown at a profit without irrigation. Alfalfa is being grown successfully in some localities in this region under irrigation, but apparently is not adapted to this section unless it can be irrigated occasionally. In 1907 a series of plantings of a number of varieties of alfalfa were made in 6-inch drills, in single rows 2 feet apart and in double rows about S inches apart, with a cultivated space of 24 inches between. These plats were not irrigated. Of the three methods of planting, the double-row method, with the intervening space kept cultivated, proved to be the best, although the yields from the plats planted in this manner were so low that they were not profitable, even during the favorable season of 1908. Six strains were also seeded in broadcast plats in December, 1906. Two of the plats died out almost entirely, the stand in many of them being reduced to 5 or 10 per cent. Several plats seeded September 25, 1906, gave much better results, the stand in October of the following year being about 90 per cent. Thorough inoculation of the soil seemed to be decidedly beneficial, the plat i Scott, John M. Japanese Sugar Cane for Forage. Bulletin 105, Florida Agricultural Experiment Station. [Cir. 1061 20 EORAOE-CROP EXPERIMENTS AT SAN ANTONIO. inoculated with soil giving much the best growth. The cuttings con- tinued so light, however, that there was not sufficient return for the expense of culture. Table XV .—Comparative yields of varieties of alfalfa. S. P. I. No. Source of variety. Yield per acre. 12549. 12846. 14786. L8627. L8628 18664. Argentina Tripoli Turkestan Arabia (irrigated seed) Arabia (nonirrigated seed; . Provence, France Pounds. 400 320 4S0 120 400 80 The best yield made by alfalfa in rod-square plats was 880 pounds an acre at one cutting. The other cuttings were only clippings made for the purpose of protecting the alfalfa from the drought as much as possible. Four varieties of alfalfa were seeded under irrigation in one-tenth of an acre plats in September, 1909. Two of these plats were almost completely ruined, and the other two badly injured by root-rot before July, 18, 1910. The root-rot proved, perhaps, more destructive than usual in these plats. POSSIBILITIES OF ALFALFA. Where alfalfa is grown in the vicinity of San Antonio under irri- gation it is generally necessary to replant the fields or portions of them every fall. Where this is clone the reported yields indicate that alfalfa is a fairly satisfactory forage crop. On the Sewer farm, south of San Antonio, five to eight cuttings are ordinarily obtained, although the last cuttings are light and mixed with native grass and weeds. Where root-rot has destroyed the plats the vacant areas are taken up by grass and weeds. It must be admitted that the possibilities of alfalfa in soils infested with root-rot are limited, and the writer has yet to see a cultivated field of any area in the black soil of this section that is not infested with this fungus. That the light, sandy soils south of San Antonio are in some instances less infested, if not entirely free from the fungus, has also been observed, but whether these isolated areas are of sufficient extent to permit the establishment of permanent alfalfa fields is yet to be demonstrated. The best superficial method of determining whether the root-rot fungus is in the soil is to observe the cotton fields. If there are small areas of varying size with dead cotton plants, the farmer will be safe in concluding that the fungus is in his fields. [Cir. 106] FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. 21 CLOVER. So far, only one variety of clover appears to be adapted to this section. This is called shaft al clover {Trifolium suaveolens). Only one test was made with this crop, but the plants made an ex- cellent growth, and this clover may have possibilities as a winter forage, although it is rather susceptible to low temperatures when not well established. Five other clovers have been tested, including two varieties of crimson clover (Trifolium incamatum) , and one variety of sweet clover (Melilotus alba). The best plat of crimson clover, which was planted in November, 1907, gave a yield of 1,240 pounds per, acre the following spring. The plat of sweet clover gave a yield of 1,640 pounds per acre. These plats were one-twen- tieth of an acre in size. Neither of the last two clovers was at all promising. COWPEAS. The cowpea is apparently as well adapted to the section of San Antonio as any other leguminous plant that has been on trial. It is of value as a forage crop, but is particularly useful as a green manure, and in a well-planned rotation there is no more valuable plant. One great difficulty with the local soil is its compactness and tendency to run together and bake. Plowing under a crop of cow- peas every three or four years will do much to make the soil more friable, and if the rotation is well planned the yields should eventu- ally be increased. The best results have been obtained from growing cowpeas in cultivated rows about .3 feet apart. No complete failure of this crop has been recorded in the five years it has been grown, and good yields have been secured under favorable conditions. Ordinarily from 1J to 2^ tons of hay per acre are obtained, although under favorable conditions the yields may be greater. Several varieties have been grown, but those that have proved best adapted to local conditions are the Whippoorwill and the Unknown. (PI. Ill, fig. 2.) Table XVI.- Yields of cowpeas without irrigation. s. P. i. No. 24919 25078 24414 17330 L'tiSKI Variety. Red Ripper droit , Brabham Chinese Whippoorwill. Unknown Yield per acre. 1909 Tans. 0. 40 .68 .72 .92 1910 Tons. 1.38 "i.93 [Cir. 106] 22 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. CANADA FIELD PEAS. For a winter legume the Canada field pea does fairly well. In the winter of 1908-9, which was extremely unfavorable, a fair crop was produced, and an excellent crop was grown during the whiter of 1907-8. This pea does not successfully withstand the hot summer weather, and consequently it can be grown profitably only as a winter crop. The peas should be planted about the first of November or earlier, in order to become well established before a severe freeze occurs. Later planting sometimes proves successful, but it is not generally advisable. It is necessary to plant early enough to have the crop mature before the hot months of the summer. In this respect Canada field peas are much like oats. A somewhat larger test was made during the winter of 1910-11, when three varieties wore grown. Owing to the severe freeze of Jan- uary all of the varieties were killed nearly to the ground and some of them were killed outright. S. P. I. No. 22042 (McKay) proved to be the hardiest of those grown. Three plats one-tenth of an acre in size were devoted to as many different methods of planting. Table XVII. — Yields in 1911 of ( 'anada field peas planted alone and with oats in 1910. Method of planting. Peas and oats mixed, proportion 1 to 5, 12-inch drills. Peas alone, 12-inch drills Peas and oats mixed, proportion 1 to 5, 24-inch drills. Rate of seeding. Pounds. 112 120 56 Yield of hay per acre. Tons. 1.23 1.94 1.16 It is doubtful whether any combination with oats will give as high a yield as when the peas are grown alone, as will be seen from the yields shown in the table. It will be observed by consulting Table XI that the peas gave a much larger yield than did any of the varie- ties of oats (PI. IV, fig. 1), and being a leguminous crop they also improved the soil on which they were grown. The hay is extremely palatable, and being rich in protein it is of value to mix with oat or sorghum hay. As a winter green-manure crop it is of particular value. A rather more extensive test of this crop was made in the winter of 1911-12. About 50 varieties bearing S. P. I. numbers were plant ed in 1-rod rows, but owing to the ravages of birds it was necessary to replant, making the resulting crop so late that the test was of little practical value. Plantings in the orchards on the farm resulted more satisfactorily. S. P. I. Nos. 18806 (Erfurt), 30307 (Canadian Beauty), and 30134 [Cir. 100] FoliAGE-CROP EXPERIMENTS AT SAN AXTOXIO. 23 (Golden Vine) were seeded with a grain drill. Nos. 18806 and 30307 were completely destroyed by the unusually severe winter, but No. 30134 came through with practically a perfect stand. The hay yield from this planting was at the rate of 2,783 pounds per acre and the grain yield 14 bushels. The hay yield in this instance does not represent what it would have been had the vines not been allowed to mature. The "vines were so ripe when cut that the greater part of the leaves dropped oil'. OTHEE LEGUMES. Table XVIII gives the yields of a number of leguminous crops tested in 1909. Table XVIII. — Yields of legumes in 1909. s. r. I. No. Variel v. Yield per acre. 212S6 22025 17534 21600 Kulthi (Dolichns biflorus) Bonavist beans (Dolichos lablab). . . do Moth beans (Phaseolus aconitifolius) Pounds. 320 4G0 520 900 Four species of Stizolobium bearing S. P. I. numbers were tested in 2-rod rows in 1910 and gave promise of being desirable legumes for this section. No. 24870 (the Bengal bean, Stizolobium aterrimum) and No. 25715 (the Florida velvet bean, S. deeringianum) were the most promising. The only objection to this legume which has been suggested is its slow growth. From the time of planting to the flowering of S. deeringianum 164 days intervened. The quantity of forage was much greater than that produced by any other legume that has been on trial. MILLET. As was noted in a previous publication, 1 millet may be used to advantage as a catch crop when an early crop of hay is desired or when the seasonal rains are very late. A crop may be matured in 40 to 60 days, and a yield as high as 1 to H tons of hay per acre has been obtained from the better varieties of early-maturing millet. The slower growing varieties give, as a whole, a somewhat larger yield per acre under favorable moisture conditions, but they are not so sure of maturing as the early ones. On the whole, millet is not as desir- able, as many other forage plants, because of its lack of drought resistance. Table XIX shows the yield-; of the varieties tested in 1908. 1 S. H. Hastings. The Work of the San Antonio Experiment Farm in 1908. Circular 34, Bureau of riant Industry, U. S. Dept. of Agriculture. 1909. [Clr. 106] u FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. Table XIX. — Number of days necessary to mature several varieties of millets grown in 1908, with yield of hay. s. P.I. No. 21533 21287 22340 22425 21601 22423 22420 22426 22427 22424 21074 22422 Valid \ . Kutki (Panicum psilopodium) Shama (Panicum colonuvfi) German ( Chaetochloa italica) do Sanwa (Panicum frumentaceum) ( 'ommon ( Chaetochloa italica) Kursk ( Chaetochloa italica) Hungarian ( ( 'haetochloa italica ) Japanese (Panicum frumentaceum) Siberian ( Chaetochloa italica) Broom-corn millet (Panicum miliaci um i do Yield per acre. Tons. 2.50 1.93 1.70 1 65 1 60 1.57 1.56 1.50 1.34 1.25 .25 .25 Pearl millet (Pennisetum americanum) was grown in 1910 under irrigation. It gave two cuttings, totaling 10.3 tons per acre, but the hay was of poor quality and the yield less than that of Sumac sorghum under like conditions. In 1 009 and 1910 tests were made, but the season was very unfavor- able and practically no hay yields were obtained. On the whole, millets are not to be recommended for use in the cropping system in the locality of San Antonio, since they are not dependable as a crop without irrigation, and under irrigation certain grasses are much more productive. GRASSES. Up to the present time no perennial grass except Johnson grass has survived for any great length of time and given a satisfactory yield in the locality of San Antonio. There is need of a good grass, especially by the irrigation farmer, owing to the fact that alfalfa, clovers, etc., are attacked by root-rot and that none of the grass family are subject to this disease. Johnson grass is grown com- mercially in a few cases, but on the whole it is rightfully considered more of a pest than a profitable crop. Owing to the nature of its rooting system it is capable of withstanding long periods of drought without dying out, but its culture should be discouraged, as there are much more profitable grasses without the objectionable features of Johnson grass which can be grown in its place. Para grass (Panicum barbinode) has been tried, and although it made a luxuriant growth under irrigation at the field station, when it was tried without irrigation it made practically no growth and completely died out during the season of 1909. The plat that was irrigated proved somewhat difficult to handle on account of the thick mass of roots which had formed during the two years the grass was being tested. The ground on which it was grown was very [Cir. 106] FORAGE-CROP EXPERIMENTS AT SAX ANTONIO. 25 difficult to plow, requiring four heavy mules hitched to a sulky plow to break the laud in the spring. This may be an objection to its being grown on a large area, unless the roots are allowed to decay before plowing. In the coast country it is being grown quite exten- sively and the reports show it to be a valuable pasture and hay grass. If the field is not irrigated for a season it is said that there i^ no difficulty in plowing the land. The plat of Para grass just mentioned was transplanted in field D3 in February, 1909, but was not irrigated that year. In 1910 this plat received eight irrigations and a hay yield of 4.65 tons was obtained. Guinea grass (Panicum maximum) has also been tried and appears to be somewhat more promising than Para grass. The plat was treated the same way as the plat of Para grass and in 1910 gave a yield of 5.29 tons per acre in two cuttings. This grass sets seed freely, while Para grass has not been known to seed hi this country. On the plat of guinea grass the stand was very thin in 1909, but in 1910 there was an excellent stand brought about by the grass reseed- ing itself. (PI. IV, fig. 2.) In July, 1910, a one-eleventh-acre plat was planted to Rhodes-gra>s (CJdoris gayana), which made a very satisfactory growth. From two cuttings 4.5 tons per acre of very excellent hay was obtained. Owing to the somewhat severe winter of 1910-11 the spring stand of grass from all these plats was very poor, as they had been badly winterkilled. As this has not happened before, there is no reason for making an adverse report on any of the varieties until more extensive trials have been made. MINOR FORAGE CROPS. LENTIL. Six varieties of lentil (Ervum lens) were planted in February, 1908. None of the varieties tested gave promise of sufficient value to war- rant extensive experimentation. MUNG BEANS. In the spring of 1908 two varieties of urd (Phaseolus max), S.P.I. Nos. 16129 and 17309, and one variety of mung bean {Pliaseohis radiatus), S. P. I. No. 16793, were tested. While these beans proved somewhat more promising than some other leguminous plants tested, they did not show indications of equaling either cowpeas or stizolo- biums, and so were discarded. CHICK-PEAS. In March, 1908, seven varieties of chick-peas (Cicer arietinum) were tested. These varieties seem not to be adapted to this district. [Cir. 106] 26 FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. GUAR. In 1906 and 1907 a plant introduced from India called guar (Cyamo- pis tetragonoloba) was tested. While this made an excellent growth and proved to be a most drought-resistant plant, the only live stock that would eat it were sheep and goats. The stalk is rather inclined to be woody, the foliage scant, and the seeds small, although it is very prolific. The fact that stock did not eat the guar may have been due in part to the age which the plants were allowed to reach before being harvested. In India the pods are said to be used as a vegetable, while in other cases it is used as a stock food or as a green-manure crop. Accord- ing to Sir George Watt, 1 when the plant is cultivated as a vegetable it is grown on highly manured land and apparently is well supplied with moisture, as it is generally planted in May and irrigated until it rains. The unsatisfactory showing made by the plant here may have been caused partly by a lack of appreciation of the requirements of the plants in order to secure the best results, as the tests were made without irrigation and during comparatively dry years. It is prob- able that the greatest value of guar for this section will be as a green- manure crop. VETCHES. About 30 different lots of vetches were tested on irrigated land during the winters of 1906-7 and 1907-8, but none of them showed indications of being a reliable winter crop. Under the most favor- able conditions the better varieties made a fair growth, but when the conditions were at all adverse, especially as to moisture supply, the growth was very unsatisfactory. Of the varieties tested, hairy vetch (Vicia villosa) and scarlet vetch (V.fulgens) made the best showing. Further trials of the vetches under irrigation are needed. SOY BEANS. In 1907 and again in 1910 several varieties of soy beans were planted and were entirely destroyed by rabbits, as the areas planted were small. Undoubtedly either large areas will have to be grown or the farm inclosed in a rabbit-proof fence if this crop is to be grown successfully. RAPE. A plat of Dwarf Essex rape was grown during the winter of 1906-7. While the plants made a strong early growth, they were later badly affected by the drought, and in February were attacked by the har- lequin cabbage bug, which entirely destroyed the crop. PEANUTS. A plat of Spanish peanuts was grown in 1908. While the plants made a fair growth, yet then' behavior in this heavy black soil and i Watt, George. The Commercial Products of India, pp. 449-450. I fir. 106] FORAGE-CROP EXPERIMENTS AT SAN ANTONIO. 27 the unevenness of the distribution of rainfall make the growing of the crop in this section inadvisable. SUMMARY. Though cotton lias heretofore been the principal crop of the San Antonio region, the increasing demand for roughage has made forage crops as profitable as cotton. ( !rop seasons are such that green forage may be available during the entire year under favorable conditions. In' the selection of crops drought resistance is of primary impor- tance; the consideration of earliness is probably second in impor- tance. Sorghum is the most widely planted forage crop of the section, and the Sumac variety has given the highest yields. Sorghum planted in rows so that cultivation is possible has given better yields on nonirrigated land than that sown in 8-inch drills. Rows 3 or 3^ feet apart are preferable to those of either a greater or less distance, yield and quality considered. Oats and rye are valuable as whiter forage. The Appier Rustproof oats have given the highest yields of seven varieties grown. Japanese sugar cane is a new forage crop well adapted to this section. Alfalfa can not be successfully produced without irrigation. On irrigated land frequent reseeding is necessary to maintain the stand on account of root-rot. Cowpeas are the best summer leguminous crop for this section. The Whippoorwill and the Unknown have proved to be the best varieties of those tested. Canada field peas have proved particularly well adapted to the local conditions in the vicinity of San Antonio as a winter crop. Millets are not as well adapted to these conditions as many other forage crops because of their lack of drought resistance. Xo perennial grasses are grown without irrigation except Johnson grass, and the growth of this is not recommended. Para grass (Panicvm l>arbinode) and guinea grass (/'. maximum) have given fair yields when grown under irrigation. Rhodes-grass (CMoris gayana) appears to be somewhat more prom- ising than either Para grass or guinea grass. Approved: James Wilson, Secretary of Agriculture. Washington, I). (\, November 9, 1912. I Cir. 106] o Issued February 7. 1913. U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 107. B. T. GALLOWAY, Chief of Bureau. A VARIETY OF MAIZE WITH SILKS MATURING BEFORE THE TASSELS. tfeJMny Ne * vo* K BY G. N. COLLINS, Botanist, Crop Acclimatization and Adaptation Investigations. 70277° fir 1(17 13 ' 10 WASHINGTON : GOVERNMENT PRINTING OFFICE : 1913 [Cir. 107] 2 BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A. Taylor. Editor, J. E. Rockwell. Chief Clerk, James E. Jones. ADDITIONAL COPIES o f this publication A may be procured from the Superintend- ent of Documents, Government Printing Office, Washington , P. C. , at 5 cents per copy B. P. I.— 799. A VARIETY OF MAIZE WITH SILKS MATURING BEFORE THE TASSELS. INTRODUCTION. In the varieties of maize cultivated in the United States the anthers usually open before the silks on the same plant appear, and pollen is still falling when the silks are in a receptive condition. Except where high and constant winds prevail at flowering time, this results in a large proportion of self -pollinated seed. In maize self-pollination so reduces the vigor of the plants that any means by which it can be avoided or reduced is worthy of careful consideration. In nature wind-pollinated species, such as maize, usually avoid self-pollination by producing pollen at a time when the stigmas of the same plant are not in a condition to be pollinated. To have the pollen produced before the stigmas are receptive (proteran- dry) or the stigmas receptive before the pollen falls (proterogyny) will prevent self-pollination. Of the two methods proterogyny would appear to be the more advantageous, and is in fact more widespread, though Kerner's statement x that "As far as we can tell at present all monoecious plants are protogynous" has been materially modified. According to Hackel, 2 proterandry is more general than proterogyny among the grasses. Of the primitive varieties of maize from tropical America that have been under observation in this country, nearly all are more decidedly proterandrous than the improved varieties grown in the United States. In spite of the fact that primitive varieties of maize are more often proterandrous than improved varieties, there are reasons for believing that the more remote ancestors of maize were proterogynous. Whether teosinte (Evchlaena mexicana Schrad.), the nearest wild relative of maize, is naturally proterandrous or proterogynous, seems not to have been recorded. In our experiments one type of Euch- laena secured from Durango, Mexico, was proterandrous to about the same extent as the unimproved varieties of maize. Samples of i Kerner, A. J., and Oliver, F. W. Natural History of Plants, vol. 2, 1895, p. 313. 2 Hackel, E. " Gramineap," in Engler and Prantl, Naturliehen l'flanzenfamilien, Th. 2, Abt. 2, p. 2. [Cir. 107 J 3 4 MAIZE WITH SILKS MATURING BEFORE THE TASSELS. Euclilaena seed from Florida, Costa Rica, and one of unknown origin imported from London produced plants that were all proterogynous. The season was too short, however, for any but the Durango plants to mature seed, and the unusual conditions may have caused the plants to behave abnormally. It is known that hybrids between maize and teosinte are common in the region about Durango. In fact, seeds that were obviously hybrids between the two species were found in the lot from which our plants were grown. It is therefore possible that although the plants showed nearly all the characters peculiar to Euclilaena, except that they were less branched, they may still be very dilute hybrids with maize, thus accounting for their proterandrous habit. The pro- terandrous habit in the Durango variety of teosinte is evidently asso- ciated with the development of a well-defined mam stalk, or culm, a character which it shares with maize and by which it differs from the other types of teosinte. Aside from the question whether teosinte is naturally proterogy- nous or not, there is evidence for the belief that the proterandrous habit of maize is the result of the separation of the sexes into different parts of the plant and that the perfect-flowered ancestors of maize were proterogynous. It is a very common occurrence in maize for pistillate flowers to be developed in the staminate inflorescence as perfect flowers or as scattered pistillate spikelets, or, more often still, for a portion of the inflorescences to be completely pistillate. An example of the frequency with which pistillate flowers occur in the terminal inflores- cence of suckers may be seen in the classification of suckers given on page 10. Another abnormality in maize of even more frequent occurrence than pistillate flowers in the staminate inflorescence is for the pistillate inflorescence, or ear, to terminate in a staminate spike. Whenever male and female flowers occur in the same inflores- cence of a maize plant as a result of either of these common abnor- malities it has always been observed that the silks are developed some days before the male flowers open. If these abnormalities are looked upon as reversions to a perfect-flowered ancestor it seems reasonable to assume that this primitive ancestor was proterogynous. ADVANTAGES OF PEOTEROGYNY. Selection for earliness and the crusade against the so-called barren stalks have without doubt operated to change the condition of pro- terandry that obtains in the unimproved varieties of maize to one of approximate synacme, where pollen and silks appear simultaneously. Synacme is certainly less desirable than proterandry, but now that [Cir. 107] MAIZE WITH SILKS MATURING BEFORE THE TASSELS. 5 we have unwittingly progressed thus far may it not be possible to proceed to the still more desirable condition of proterogyny % Self-pollination, while less desirable, is at the same time less precarious than cross-pollination. It seems to be an inefficient arrangement to have the opportunity for the deleterious (but rela- tively more certain) self-pollination precede the opportunity for the advantageous (but more precarious) cross-pollination. If the conditions could be reversed by having the plants moderately pro- terogynous, the proportion of cross-pollinated seed would be greatly increased, while if by any chance the silks failed to receive foreign pollen, pollen from the same plant would be forthcoming as a last resort, and the danger of poorly filled ears would be avoided. The advantages of proterogyny in maize appear so important that the effort has been made to develop this habit in some of the improved varieties. Occasional proterogynous plants are to be found in most varieties, and wherever such plants have been observed in our experiments seed has been saved and planted the following year. In many instances it was found, however, that the proterogyny was only apparent, resulting from an abnormal development of the staminate flowers, and many of the plants noted as proterogynous proved to be sterile as far as the production of pollen was concerned. As with other characters that result from injury or abnormal condi- tions, proterogynous variations of this nature have been found to be inherited very imperfectly, if at all, and little progress has been made by this method. The discovery of a variety that appears to be regularly proterogynous makes the outlook for improvement in this direction much more hopeful. Two ears of a red pop corn, purchased at Granada, Spain, by Mr. Waiter T. Swingle, of this Bureau, in January, 1912, and planted at Lanham, Md., the following spring, produced plants, nearly every one of which was proterogynous. The ears of this variety are so small that the variety will probably be of no economic importance except perhaps as a pop corn. The subsequent behavior of the variety can not be safely inferred from its behavior the first year, but that the proterogynous tendency exists to a marked degree seems certain, and to attempt its transfer to our improved varieties would seem well worth the effort. The present account is published in the belief that those interested in developing new types of maize will find valuable breeding material in this strain. Only a small quantity is on hand, but a few seeds can be supplied to those wishing to make a study of this variation and to assist in deter- mining its economic importance. The improvement of maize varieties should be looked upon as a local problem. The acclimatization of an improved variety is likely to entail nearly as much time and labor as the original work of improve- [Clr. 107] MAIZE WITH SILKS MATURING BEFORE THE TASSELS. ment. Before the value of a variety possessing novel characteristics can be determined it should be tried in a great variety of different conditions and in combination with different varieties. DESCRIPTION OF THE VARIETY. The two original ears secured by Mr. Swingle, though apparently belonging to the same general type, showed obvious differences. Ear No. 1 (fig. 1) was 12 cm. long, 13 cm. in circumference, rather abruptly tapering, and with 20 somewhat ir- regular rows of small, rounded grains. The pericarp was wine colored or gar- net. A few of the seeds of this ear had a colored aleurone visible beneath the colored pericarp. The stigmas were persistent after the manner of the "rice" varieties, but the seeds were not beaked. The cob was a dirty red. Ear No. 2 (fig. 2) was 16 cm. long, 10.5 cm. in circumference, more nearly cylindrical, and with 16 regular rows. The pericarp was much lighter than in ear No. 1, almost a brick red. The stigmatic point was apparent, but no portion of the stigma remained at- tached to the seed. The cob was col- ored, but was of a lighter shade than that of ear No. 1. Ears Nos. 1 and 2 have been num- bered 34426 and 34427, respectively, in the seed and plant introduction series of the Department of Agriculture. Seeds from each of the ears were planted in separate rows and 62 plants grew to maturity. The plants were strong, vigorous, and unusually uniform (fig. 3). Aside from the proterogynous habit, the most striking characteristic of the type was the dense clustering of the spikelets toward the end of the branches of the tassel. To make pos- sible a quantitative statement of this tendency, the uppermost branch of the tassel of each plant was divided at the middle and the spikelets of the two halves counted. It was found that on the average [Cir. 107] 5B*ffi«!^«a Fig. 1.— Proterogynous variety of maize. Ear No. 1. (Natural size.) MAIZE V/ITH SILKS MATURING BEFORE THE TASSELS. the distal half of the branch bore 2.13 times as many spikelets as the prox- imal half. It was in connection with this va- riety that tins character was first noted, and consequently the data for making comparisons with other varie- ties are meager. Measurements of 50 plants taken at random from a neigh- boring field of Boone County White corn showed the distal portion to have only 1.33 times as many spike- lets as the proximal portion. In other varieties the distribution of the spike- lets is still more nearly uniform. The increase in the number of the spikelets toward the end of the branches is brought about by a change in the arrangement. In the basal portion of the branches the arrangement is normal, that is, the spikelets are arranged alter- nately in pairs, each of which consists of a stalked and a sessile spikelet. Toward the end of the branches all the spikelets are sessile and the number in each group increases from two to three or more. Another peculiarity, perhaps asso- ciated with the proterogynous habit, is that the silks in most of the plants, instead of appearing first on the upper- most ear, appear almost simultaneously from three ears, and in a number of plants the silks of the second ear showed before those of the first ear. Daily notes were taken on the indi- vidual plants, and the flowering time of both male and female flowers was noted. Of 59 plants, 8 had sterile tassels and failed to produce pollen. Of the remaining 51 plants, in 1 the anthers opened two days before the [Cir. 107] *J* 1 ft v^ % s V Qc% Or? 4* Ft Fig. 2.— Proterogynous variety of maize. Ear No. 2. (Natural size.) 8 MAIZE WITH SILKS MATURING BEFORE THE TASSELS. silks emerged, in 9 the silks appeared on the day the first pollen was shed, and in 41 silks were produced from 1 to 7 Fig. 3. — Plant of a proterogynous variety of maize. days before pollen fell. The average number of days that elapsed after the silks emerged before pollen was shed was 2.1. The rela- tor. 107] MAIZE WITH SILKS MATURING BEFORE THE TASSELS. 9 tive frequency of the different degrees of proterogyny is shown below : Number of days after the silks ap- peared before pollen was shed — 2 —1 Number of plants 1 9 12 3 4 5 6 7 7 13 13 5 2 1 Of the 29 plants grown from ear No. 1, the pericarps of 9 were white, the remaining 20 being colored as in the parent ear. Of the 33 plants grown from ear No. 2, the pericarps of 11 were white, the remaining 22 being a red of the same shade as the parent ear and consistently lighter than the progeny of ear No. 1. The measurements shown in Table I are given with the idea of providing data for determining the effects of acclimatization. The size of a plant and of its various organs is so directly dependent on the nature of the environment that measurements are of little value for descriptive purposes except as they are made comparative by including familiar varieties that may be used as a standard. If, however, the expression of characters has been seriously disturbed by the change of locality, the number of the different parts as well as the actual dimensions may be expected to change in subsequent generations, and the data here presented may serve as a rough indi- cation of such comparative relations. Since in a number of char- acters the differences appear to be significant and not the result of chance variation, the average measurements of the plants grown from each of the two ears as well as the combined averages are recorded separately. Table I. — Measurements of plants grown, from, two ears of red pop com from Spain. Character. Height centimeters . Number of nodes above the ground Number of nodes alio ve the ear Numl >er of green leaves at silking Length of longest leaf centimeters. Width of longest leaf do. Number of nodes above longest leaf Distance from top of last leaf sheath to the lowest tassel branch centimel srs - . Distance from first to last tassel branch do. . . . Distance from last tassel I iranch to tip of tassel do Number of tassel branches Number of secondary 1 irancb.es in tassel Length of uppermost tassel branch centimeters. . Number of spikelets on uppermost tassel branch Length of staminate spikelets millimeters. . Number of ears per plant Number of rows in upper ear Number of rows in second ear Length of upper ear centimeters. . Number of suckers per plant Ear No. 1. 249 ±3.55 15.4 ± .12 4.93± .08 14.5 ± .16 99.8 ±1.14 10.1 ± .13 10 ± .16 3.55± .38 13.8 ± .29 29.9 ± .48 21.6 ± .03 6 ± .39 9. (if 2.5 18.3 19.4 19.3 1.0 .1(1 .08 .19 .42 .46 .19 Ear No. 2. 226.5 ±2.57 14.3 ± 4.26± 13.8 ± 94.6 ± 9.6 ± 9.2 ± .12 .1 ,09 .88 09 13 3.2 15 27.9 26.6 • 7.4 18.2 82.8 ± ± ± ± ± ± ± 9.78± .16 2.8 ± .14 15.8 ± 17.3 ± 18 ± 2.1 ± .31 .31 . 30 .76 .48 .17 . 32 .10 .27 .37 .18 Combined average. 238.5 ±2.34 14.8 4.6 14. 1 97.4 9.8 9.6 9. 73 ± 2.7 ± 16.2 ± 18.8 ± 18. 6 ± 1.8 ± .07 .07 .09 .75 .07 .11 3.4 ± .25 14.2 ± .22 29 ± .30 23.9 ± .59 6.5 ± .30 07 13 13 24 23 10 [Cir. 107] 10 MAIZE WITH SILKS MATURING BEFORE THE TASSELS. ABNORMALITIES. The nature and relationships of a variety are often best determined by a study of the abnormalities which the plants exhibit. Though usually more pronounced the first year that a variety is grown in a new locality, the relative frequency of the different abnormalities is often significant. The following abnormalities were observed in the plants grown from both ears: Husks coalesced, 2; fascicled spikelets in tassel, 3; lobed husks, 5; ears with staminate flowers at tip, 4; fasciated (or "bear's-foot") ears, 9; ears exserted beyond husks, 4. In addition to these common abnormalities that occur with varying frequency in nearly all varieties, single examples of the following were discovered: A leaf like bract in the tassel; a small ear inclosed in husks at the base of the tassel; perfect flowers in the ear (in this plant three well-defined stamens were found at the base of nearly eveiy seed — an abnormality which may have been overlooked in other plants, since the presence of less well-developed stamens could have been determined only by carefully dissecting each ear); two seeds grown together in the ear; 1 and a secondary ear borne directly in the axil of the prophyllum instead of the axil of a husk, a phenomenon of rare occurrence in maize, except in the hairy Mexican type (Zea hirta), in which it is normal. The above abnormalities were confined to 21 plants. In the re- maining 38 plants no abnormalities were observed. Great diversity was exhibited in the suckers, a characteristic of unimproved types, especially when grown in a new locality. The series was so extensive that an attempt was made to classify the different types, the result of which is shown in Table II, together with the relative frequency of the different types. These variations might be classed as abnormalities, but they all represent intermediate stages between the two normal types of branches, viz, the pistillate inflorescence (or ears) and normal suckers that are replicas of the main stalk. Table II. — Classification of suckers. No. Description of sucker. Number of plants. Resembling main stalk, ear and tassel normal Similar to No. 1, with no ear developed Terminal infloresence staminate, with tips of central spike and branches aborted; no ear. . Similar to No. 3, with ear in normal position Terminal inflorescence staminate, with scattered pistillate flowers on both central spike and branches; no ear Similar to No. 5, with ear in normal position Terminal inflorescence staminate and normal except for one pistillate branch; no ear Terminal inflorescence staminate and normal except for a pistillate portion at tip of cen- tral spike; no ear Similar to No. 8, but with ear in normal position Terminal inflorescence staminate except for a pistillate portion at the base of both central spike and branches; no ear 17 20 4 1 1 1 1 11 1 1 This abnormality has been described and its significance discussed by Mr. J. H. Kempton in " Two- Flowered Female Spikelets in Maize," Bulletin 278 of the Bureau of Plant Industry. [Cir. 107] MAIZE WITH SILKS MATURING BEFORE THE TASSELS. 11 Table II. — Classification of suckers — Continued. No. n 12 13 14 15 16 18 19 20 Description of sucker. Terminal inflorescence staminate except for a pistillate portion at base of central spike and tips of branches; no ear - - ;---.;---:- / - - ■ Terminal inflorescence staminate, with pist Mate portion at base of central spike; branches wanting; no ear - .•--.----; :----.;--■ Terminal inflorescence with pistillate portions at the base and tip of central spike; branches staminate; no ear Terminal inflorescence with central spike completely pistillate; branches stammate, or nearly so; no ear - Similar" to No. 14 except for a staminate portion at tip of central spike Terminal inflorescence with central spike completely pistillate; branches staminate, with pistillate portion at tip; no ear Terminal inflorescence with central spike completely pistillate: branches pistillate, or nearly so; no ear Terminal inflorescence pistillate; no branches; no ear Terminal inflorescence a normal staminate tassel, with an ear at its base; no ear in normal position No terminal inflorescence; no ear Number of plants. The suckers which resembled main stalks, producing; both ears and tassels, were also for the most part proterogynous, though less uni- formly so than the main stalk. Notes were secured on the suckers of 15 such plants. Of these, two were proterandrous, one was synac- mic, two had one sucker proterogynous and one proterandrous, four had sterile tassels, and eight were proterogynous. In every plant with sterile tassels on the sucker the tassel of the main stalk was also sterile. There were apparently no intermediate stages between completely sterile tassels and those producing an abundance of pol- len. Sterile tassels are not uncommon in many varieties of maize which show no proterogynous tendencies. SUMMARY. The present paper records the discovery of the proterogynous habit in a variety of maize introduced from Granada, Spain. In plants of this type the silks are exserted and receptive before the pollen begins to fall. This proterogynous characteristic insures a much larger percentage of cross-pollinated seed than is obtained in the ordinary varieties, in which the falling of the pollen is simulta- neous with or precedes the exsertion of the silks. If this character can be combined with the good qualities of improved American types it will obviate the necessity of detasseling to secure cross-pollinated seed. A small quantity of pure seed of this variety was produced during the past season and is available for those wishing to undertake the introduction of this character into local varieties. Approved : James Wilson, Secretary of Agriculture. Washington, D. C, November 21 , 1912. [Cir. 107] o Issued April 5, 1913. U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 108. B. T. GALLOWAY, Chief of Bureau. THE CHINESE WOOD-OIL TREE. BY DAVID FAIRCHILD. Agricultural Explorer in Charge of Foreign Seed and Plant Introduction. WASHINGTON : GOVERNMENT PRINTING OFFICE : 1913 [Cir. 108 2 BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A. Tayl Editor, J. E. Rockwell. Chief Clerk, James E. Jones. Cir. 108, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate I. ■ l .! An Old Wood-Oil Tree 30 Feet High in Full Flower in Western Szechwan, China, on the Banks of the Yangtze River. (Photographed by Mr. E. II. Wilson, Arnold Arboretum Expedition, 1908.) B. P. I.— 802. THE CHINESE WOOD-OIL TREE. INTRODUCTION. The Chinese wood-oil or tung-oil tree (Aleurites fordii) is grown on the heavy-clay hillsides and waste places along the Yangtze River above Hankow, where the rainfall is heavy and the climate is similar to that of some of our Southern States, although the temperature does not go so low in winter. The tree is probably not very long- lived and would be comparable in this respect to the silver maple. It drops its leaves in winter and does not wake up early in the spring, like many trees, and therefore is not likely to be severely injured by late frosts. The flowers come out before the leaves. They are fully as large as catalpa flowers, and the tree in bloom is a very pretty sight. As an ornamental the wood-oil tree (Pis. I and II) is likely to prove about as desirable as the catalpa, but the soft wood is of little value and, like many other soft-wooded trees, the branches break off easily in heavy winds. The Chinese wood-oil tree commences to bear fruit when 4 or 5 years old. The fruits are the size of small apples — from 2 to 3 inches in diameter. (PI. III.) They contain from two to eight (most commonly five) large, oily seeds, that are reported to be poisonous and should not be eaten. They at least have a purgative effect, similar to that of the castor bean, to which the wood-oil tree is botanically distantly related. The yield of these nuts in China is reported to be from 30 to 75 pounds per tree. ECONOMIC VALUE OF WOOD OR TUNG OIL. The value of this Chinese tree lies in the fact that the nuts contain one of the best drying oils known, called wood or tung oil. In recent years this oil has produced, it is reported, a revolutionary effect on the varnish industry of the United States. It has largely taken the place of kauri gum and has made possible the manufacture of a quicker drying varnish, which is less liable to crack than that made from kauri gum, and has been found of special value in waterproof priming for cement. 72160°— Cir. 108—13 3 4 THE CHINESE WOOD-OIL TREE. This valuable oil constitutes about 23.9 per cent of the substance of the nuts, and its market price, which is normally 6 to 7 cents per pound, has risen recently to 12 cents. If figured out on the basis of 1 pound of the applelike fruits yielding 0.58 pound of nuts and 0.138 pound of oil, a pound of fresh fruits would be worth from 0.82 cent to 1.6 cents, and a bushel, which would weigh approximately 27 pounds, would be worth from 22 to 43 cents, depending on the market price of the oil. For comparison, 65 cents a bushel is considered a fair price for apples. Not more than 108 trees (i. e., 20 by 20 feet apart) should be planted to the acre, and this would mean a possible gross yearly return of from $23 to $46, depending on the market price of the oil figured on the above basis. One 8-year-old tree belonging to Mr. W. H. Raynes, of Tallahassee, Fla., bore this year 852 fruits, prac- tically 2 bushels, which would make the gross returns from $46 to $92 an acre, provided that all the trees in an orchard did as well as this one. CULTIVATION. In the neighborhood of Tallahassee, Fla., land suitable for the cultivation of the Chinese wood-oil tree is selling for $10 to $15 an acre, and land on which it will grow can probably be had for much less. The cost of planting, cultivation, and marketing would prob- ably not exceed $15 an acre yearly, so that as a tree crop this wood-oil tree is worthy of consideration by the owners of cheap lands in the South. Experiments might be made in blasting holes for the reception of the trees where soil conditions render digging difficult in the ordinary way. Since the trees are rapid growers, it is also probable that it will be satisfactory if the soil is kept free of weeds for a space of 4 feet on each side of the row and the center space between the rows left in sod. After an orchard is well established it may not require any cultivation, although possibly the increased crops would more than pay for the cost of clean culture. PRODUCTION OF WOOD OIL. The work of gathering these large fruits will not, in all probability, approach the cost of gathering apples, which is commonly 5 cents a bushel. The nuts are removed from the husks very readily after the fruits have been stacked in heaps, and the process of crushing and extracting the oil is not likely to be more expensive than the ex- traction of cottonseed oil. It is also probable that the same or similar machinery can be used for the expression of wood oil. In China, according to reports, after the fruits have been gathered and the husks removed, the nuts are put into a large iron pan about [Cir. 108] Cir. 108, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate II. Wood-Oil Tree in Full Bloom, near Tallahassee, Fla., on the Estate of Mr. William H. Raynes. This tree was imported as a seed from Hankow, China, by Mr. L. S. Wilcox in 1905. It bore 410 fruits in mil and 852 fruits in 1912, approximating 1 and '2 bushels, respectively. It lias not been injured by a temperature of 14° F. Cir. 108, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate III. CO +3 CO ta 2 '53 - •"*■ X >, O rQ _i t- _; o ~ DO t- .— co ~ " z 7*r- *: i-S a. s ^ O O at I ^.^2 1* CO C° < t. CH ci~* cr ;. '"■ 2 K a u_ S- O Cy CD J= > LU -w ^ r- _, CD < X ~ r- co LU M -X3 io UJ I i'd 1- z *=■ si o o z f & 5 %w o ^H rr £fl a 0) 0) „ /— _£; UJ +j +-» u u~t *y rr r-^ O H o "t^ £ z o o 3 -s p. 1- .1^ tr Bo O ~ *-* GO _J m" o o3 ■u o Q mJ- o „&* o c £ o • "T'o LU "'SO co UJ 03 S z S -^ s-. . I 2 S o ^ •— < 0/ C u_ .■z — o C3 CD 43 x r- C CO o & h Sp z> »: O £E .ti ° u. p z f— H UJ CD Ul X rr O p THE CHINESE WOOD-OIL TEEE. 5 18 inches in diameter and are stirred over a fire until parched. The seeds are then ground into a fine meal, which is heated or steamed before it is put in the press, supposedly for the purpose of assisting in the extraction of the oil. POSSIBILITIES OF THE WOOD-OIL INDUSTRY. In starting an oriental industry in America the most important factor to be considered is the amount of hand labor involved. There does not appear to be much involved in this industry, as the gathering and husking of the fruits seem to be the only hand work required. The freight haul from China to America is a water haul practically all of the way, but instead of its acting as a tariff wall to protect the grower in this country it apparently acts as a handicap, at least at the beginning of such an industry. According to recent freight rates a grower in Montgomery, Ala., routing his product to Mobile by rail and thence by water to New York, would have to pay 86 cents per 100 pounds, whereas his Chinese competitor at Hankow pays only 61.5 cents per 100 pounds to ship around the world to New York. However, it is not to be expected that these rates would be maintained in case the supply of oil became an important article of commerce. There enters into this discussion, however, the factor of a home supply of the material, and undoubtedly this is very desirable. The American farmer has the advantage over the Chinaman of cheap, accessible lands and team labor. Since the hand labor involved in a well-planned orchard is not great, it would seem to be entirely possible by the systematizing of such an industry on large plantations to produce this wood oil more cheaply than it is now produced by the wayside plantings in China, which must be very wasteful of human labor. This labor factor in China is now becom- ing an important one, as the cost of labor is rapidly rising. It seems reasonable, therefore, to suppose that the American extensive method of handling such a tree crop would in time overtake and outstrip the back-yard and wayside methods of the Chinese. The prospects are that there will be a continual and growing demand for wood oil. Five million gallons were imported from China last year. The growing use of soy-bean oil, it is reported, will tend to increase rather than decrease the consumption of wood oil, as soy- bean oil dries too slowly and requires an addition of wood oil to help it dry. The home demand in China is likely to increase and the opinion of importers seems to be that the American-grown oil could capture the market. If it does, 40,000 acres of trees would be required to supply the present demand. There now remains to be considered the very important question whether the American-grown trees will produce as good a product as |Cir. 1081 6 THE CHINESE WOOD-OIL TREE. the Chinese trees. This problem still remains to be determined, but will doubtless be settled in the course of a few months. There seems to be no basis for the theory, however, that the oil produced here by the same species of tree will be materially different from that produced in China. Whatever differences have already been observed in the small samples which have been expressed from the nuts pro- duced in Florida are attributed by Dr. Rodney H. True, of the Office of Drug-Plant, Poisonous-Plant, Physiological, and Fermentation Investigations, to the method of extraction of the oil rather than to any specific difference in its composition. SUMMARY. To sum up the whole wood-oil situation as it stands to-day in the minds of investigators of the Department of Agriculture: An important plant industry, involving a present area of 40,000 acres and a possible one of several times this acreage, is here repre- sented. Chinese wood-oil trees have grown and fruited well in South Caro- lina, Florida, Alabama, Louisiana, Mississippi, Georgia, Texas, and California. These trees are growing on cheap land and do not require very careful attention. The tree has stood a temperature as low as 4° F., at Clemson College, S. C, without injury, except the loss of a few of the small lateral branches, and is slow to start into growth even when subjected to a temperature of 80° F. It is therefore not so liable to be injured when this temperature is immediately fol- lowed by a drop to 18° F. There are large regions in the South where the temperature for decades at a time does not go below zero Fahren- heit, and it is in these that it should be tried. Until it is known just how low a temperature the wood-oil tree will stand without injury it is not safe to predict the northern limit of its probable cultivation. The distribution of several thousand wood-oil trees through the South in 1906 and 1907 has brought in a considerable amount of infor- mation as to the behavior of the tree in this country. From these data it appears that the tree has done best in the more moist parts of the Gulf coast region, on deep loam soils which are underlain with stiff clay. The sticky gumbo soils of eastern Texas seem unfavorable to its growth, and it has not done well on the almost pure sand soils of Florida. The wood-oil tree quickly recovers from frost injury, sprouting up again from the stump. Being of rapid growth, it should recover more quickly than an orange tree. The indications are that the blooms may be occasionally caught by late frosts, as they open in March in the latitude of Tallahassee, Fla., but in this respect they seem to be less liable to injury than pear and peach trees. [Cir. 108] THE CHINESE WOOD-OIL TEEE. 7 The Department of Agriculture will have on hand one year from now for distribution to bona fide experimenters a limited number of 1-year-old trees, and these will be ready to send out in February and March. Applicants for them must have the necessary conditions of soil, temperature, and rainfall and the actual intention to take up the serious study of this industry before their requests for plants can be granted. Experiments with single trees have been made, and what is now desired is the creation of acre plantations in the hands of private individuals. Approved : James Wilson, Secretary of Agriculture. Washington, D. C, November 29, 1912. Cir. 108J ADDITIONAL COPIES of this publication -£*- may be procured from the Superintend- ent of Documents, Government Printing Office, Washington, D. C, at 5 cents per copy U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 109. B. T. GALLOWAY, Chief of Bureau. MISCELLANE( )US PAPERS. United States Official Cotton Grades N. A. COBB Potato Leaf-Roll • . W. A. ORTON Morphology of Cotton Branches 0. F. COOK The Wilting Coefficient for Plants in Alkali Soils T. H. KEARNEY Interpreting the Variation of Plat Yields F. D. FARRELL RK Issued January 4, 1913. WASHINGTON: GOVERNMENT PRINTING OFFICE. 1913. BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A.Taylor. Editor. J. E. Rockwell. Chief Clerk, James E. Jones. [Cir. 109] 2 UNITED STATES OFFICIAL COTTON GRADES. 1 By N. A. Cobb, Agricultural Technologist in Charge of Agricultural Technology and Cotton Standardization and Grading Investigations. AUTHORIZATION AND SALE OF STANDARD COTTON GRADES. The appropriation bill for the Department of Agriculture for the year ended June 30, 1909, authorized the fixing of a standard of middling cotton and, using the same as a basis, the establishment of nine grades of cotton, to be designated — Middling fair. Strict good middling. Low middling. Strict middling. Middling. Strict good ordinary. Good middling. Strict low middling. Good ordinary. The Secretary of Agriculture was authorized to call to his assist- ance expert cotton classers, and he was directed to prepare copies of the official standard in practical form for distribution at cost. The Secretary convened in Washington a committee of prominent cotton growers, dealers, manufacturers, and experts in February, 1909, and after due deliberation the committee submitted a unani- mous report, together with a set of grades which they had prepared in accordance with the wording of the appropriation act. This report was approved by the Secretary. To familiarize the public with the official grades, copies of them were temporarily placed with a number of associations, exchanges, col- leges, and other organizations for examination. Within six months after their promulgation sets had been purchased by cotton exchanges, cotton mills, and textile schools in 21 States, as well as in England, Germany, and Mexico. The sales of the grades have doubled in each succeeding year, so that at the present time they are distrib- uted in 40 States and 10 foreign countries, and have been officially adopted by the following cotton exchanges and associations: New Orleans. Little Rock. New England Buyers. Memphis. Galveston. Arkwright Club. St. Louis. Macon. Southern Cotton Buyers. Charleston. Mobile. Fall River Cotton Buyers. Natchez. Oklahoma. i Issued Jan. 4, 1913. [Cir. 109] 3 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. The grades are in use in a number of other associations that have not gone through the formality of adopting them by vote. The accom- panying chart indicates the distribution of the grades up to the 1st of October, 1912. The original set of standards was prepared by experts under the direction of the committee referred to, and from this set the copies for sale have been made by experts from time to time, as required. The original cost of preparing a complete set of the grades was $35, but within the past year it has been reduced to $25. A set DISTRIBUTION Official Cotton Gmdes of the US'Depdriment ofAcriculfure The United States Official Cotton Grades have been on sale for two years and one month. The present price is §25 per set of nine grades. The chart does not shon the distribution abroad. The Grades have been sold in Great Britain, Ger- many. France, Italv. Belgium, Japan. India, and Mexico. The Official Grades are prepared and issued by the Secretary of Agriculture in accordance with law. The Department has no power to dispose of the Official Grades except by sale. of the grades consists of 9 boxes, each containing 12 samples, sepa- rately packed, which show the range of diversity within the grade. In the top of each box is a photograph showing the appearance of the cotton when certified by the Secretary. Fractional sets are sold practically pro rata. PERMANENCY AND VALUE OF STANDARDIZATION. One of the problems which confronted the department at the beginning was the preservation of the grades as originally prepared by the committee, and numerous experiments were made to deter- mine the best plan to be followed. The experiments indicated that LCir. 109] UNITED STATES OFFICIAL COTTON GRADES. 5 by the use of large vacuum tubes the cotton types could be kept without appreciable change for an indefinite period of time. Fifty sets of the grades have been put up in vacuum tubes and stored for future use. Each separate sample in each grade was placed in a vacuum tube, making 12 tubes for each grade, or 108 for a complete set. The cotton is first wrapped in chemically pure wliite paper and then in pure black paper, before being placed in the tube, and in the top of the tube, after the insertion of the wrapped cotton, a 2-inch layer of specially prepared asbestos is placed. The air is then exhausted and the tube sealed by fusing off. No such system has ever before been put into effect in connection with cotton grades or any other graded agricultural product. This is probably one of the largest undertakings of the kind ever attempted. From time to time one of these vacuum sets will be broken open and replaced in grade boxes, each sample having been specially pre- pared and labeled with that end in view. In this way absolutely exact copies of the original types will be available from time to time for the next 100 years or more, and cotton grading will, for the first time, be freed from the drawback of depending solely on the memory of experts. A campaign to educate the producers and local buyers in the use of the grades has been carried on through the agents in charge of the Farmers' Cooperative Demonstration Work. Several of the agricultural colleges are now teaching cotton grading at their summer schools, as well as during the regular term, and are using the official grades as the basis of instruction. With the return of the students to the farm a demand for the grades for use on the farm is bound to grow. With the increasing quantity of cotton which is now sold by the grower directly to the mill, the value of the grades is increasing, all branches of the industry, producer, merchant, and manufacturer alike, using a uniform standard whose correctness and constancy are guaranteed by the Government. When the original set of types was prepared by the committee appointed for the purpose, the aim was to produce a set which would be representative of American white cotton as a whole. No atten- tion was paid to the source from which the cotton came, and this policy has been continued in the preparation and sale of copies of the original types. The committee had before it an exceedingly great variety of American cotton. Not only did the department secure, through the officials of about 30 American cotton exchanges, grade types in accordance with the ideas of those exchanges and place these entirely at the disposal of the committee, but members of the committee were invited to bring with them cotton winch they con- sidered would be suitable for the preparation of the official grades. [Cir. 109] 6 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. A number of members provided cotton in accordance with tins invi- tation. It is safe to say that the committee's attempt to make a set of grades that would apply to the whole of the American cotton belt was so sincere and so adequately backed up by suitable material from which to prepare the grades that the result was all that could possibly have been expected. During the past few months an effort has been made to ascertain what proportion of the 1912 crop is classifiable on the basis of the present official grades. From a collection of many hundreds of sam- ples taken at random from various parts of the cotton belt, very little cotton has been secured which was not readily classifiable on the basis of the present official grades. There is a tendency toward the increased use of cleaning machines as adjuncts to ginning machinery. . These cleaning machines remove trash and dirt and thus raise the grade of the cotton, so that in the future we may expect more and more cotton to fall into the higher grades, where there is less difference between the cottons from various parts of the country. This tendency toward cleaner cotton will doubtless facilitate the use of the official grades. It is important to keep in mind that many people, in the practice of grading cotton, are influenced, consciously or unconsciously, by other factors than the color and the amount of trash. Where such a practice prevails, a sample of cotton that on the basis of color and trash would pass as official middling, may pass as of a higher grade, if the staple is unusually good. It is believed that such grading can result only in confusion and that length of staple should be entirely dissociated from the official grades. FURTHER WORK IN CONTEMPLATION. The officers of the department are carefully investigating the various problems connected with the question of cotton standardiza- tion, and whenever the time seems ripe for further steps toward the establishment of universal cotton grades the department will be equipped and ready to give all the assistance possible. If the plan of establishing standards for the grading of American cotton is to be brought to a successful issue, it is of the utmost importance that the foundations upon which these standards rest shall be secure. The firmest foundation possible will be that which is in thorough accord with present practices, and the wish of the officers of the department is that no mistakes be made in this funda- mental work. If a foundation can be laid which meets with univer- sal approval there is not the slightest doubt that there can be built upon it a system of cotton standardization which will be of great service throughout the cotton industry. [Cir. 109] POTATO LEAF-ROLL. 1 By W. A. Orton, Pathologist in Charge of Cotton and Truck Disease and Sugar-Plant Investigations. INTRODUCTION. The years 1911 and 1912 have been marked by the prevalence of potato troubles in some of our Western States more serious than any hitherto experienced. Such heavy losses have resulted, particularly in eastern Colorado and western Nebraska, that the production of potatoes, generally one of the most profitable crops for these irrigated districts, has been rendered so uncertain that the growers have been compelled to greatly curtail their acreage. The Bureau of Plant Industry has nearly ready for publication a bulletin on these new diseases, but as this will necessarily be some- what technical in character and not available for distribution until later in the season, this paper is issued for the information of the potato growers in the districts affected. The identification of the disease responsible for the decrease in potato production in the country east of the Rocky Mountains and west of the one hundredth meridian has been in doubt until recently. It was at first thought to be an aggravated form of diseases already known, Rhizoctonia, stem-blight, and Fusarium wilt, but it is now considered a new and distinct malady called "leaf- roll." This has been common in Europe since 1905, but has not hitherto been iden- tified in the United States. DESCRIPTION OF LEAF-ROLL. As indicated by the name, leaf-roll is marked by an upward rolling of the leaves on then- midrib. There is usually a pronounced change in the color of the foliage to a yellow, unhealthy shade, often tinged reddish or purplish. The disease may start early in summer and be far advanced by the end of July. The plants do not die quickly, as they do when attacked by Fusarium wilt, but may live nearly as long as healthy oil es. The growth is checked and the formation of tubers prevented. Often no potatoes are set, or only small ones clustered around the i Issued Jan. 4, 1913. [Cir. 109] 7 S CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. base of the stem, while numerous rudimentary tubers are formed on the stolons. The browning of the woody part of the potato stem and the pres- ence of a brown discolored ring at the stem end of the tubers is not so much a character of the leaf-roll, but is rather to be taken as an indication of the presence of another disease, the Fusarium wilt. The formation of aerial tubers is sometimes a feature of leaf -roll, but in other cases is the result of stem cankers caused by the fungus Rhizoctonia. Leaf-roll is considered to be hereditary through the seed potatoes; that is, if potatoes borne on plants affected by leaf-roll are planted the resulting crop will be diseased and usually much worse than the first crop. The cause of leaf-roll remains unknown, though it has been pre- valent in Europe smce 1905 and has been given much study there. It is now believed to be a physiological disorder rather than one caused by a parasite. Many consider it due to some unfavorable soil or climatic condition, but no one has been able to show what conditions produce it or how it may be controlled by any cultural practices. LEAF-ROLL AN UNSOLVED PROBLEM. No fully satisfactory remedy for leaf-roll has been discovered. It presents one of our most serious problems for investigation and one which it is hoped to push actively as soon as means are provided. We have, however, the benefit of seven years of German experience with the same trouble. The anxiety caused by its appearance in Germany has been somewhat allayed with the passage of time, and the best authority on potato diseases there even states that through the awakening of interest in better culture and in improvement of seed the leaf-roll will prove in the end a benefit to German agricul- turists and their potato production will be permanently increased. CONTROL MEASURES. While we can not recommend any preventive treatment with the confident assurance that it will be successful, there are some points of attack that are strongly to be recommended as having given the best results elsewhere and as being common-sense measures whether disease is present or not. Most important of these are good seed, crop rotation, and improved culture. The seed problem takes first place in any movement for the better- ment of our potato industry and particularly in these western dis- tricts, where diseases are extensively carried on seed. While there may be occasional apparent exceptions, it is the general rule that seed from fields affected by leaf -roll will give a diseased crop. It is [Cir. 109] POTATO LEAP-ROLL. 9 therefore strongly to be recommended that no seed be planted except that known to come from healthy fields. If there are none in the neighborhood, seed should be brought in from outside. Leaf- roll is not known to occur in Minnesota, Wisconsin, or Michigan. The expense of. bringing seed from distant points and the uncer- tainty of getting a vigorous stock of the variety desired emphasize the great need of a better organization of the potato growers for seed selection and inspection. It should be possible to buy seed potatoes accompanied by a certificate from a reliable authority that they are free from disease and of the variety claimed. Such certification should be based on a field inspection made in early autumn, when the foliage is still alive. Leaf-roll can not be detected by an inspection of the tubers. In purchasing seed potatoes, those infected with Fusarium wilt should also be avoided. Any lot where many tubers show a brown f discolored ring when cut across the stem end should be discarded. Crop rotation is absolutely essential to permanent potato culture. It is a common practice to grow several successive crops in new western land, but this always has one inevitable result — diseases are introduced and spread until it is no longer possible to grow potatoes with profit. Must every community and every farmer learn this lesson separately, or will the experience of the many profit the remain- ing few ? There is abundant land in every irrigated district for a long rota- tion, and particularly at this time when so few potatoes are being planted it is possible to put them on land that has not been in this crop for four or five years. Cultural methods may reduce the injury from leaf -roll and other diseases. No pains should be spared to give the potato crop the care and attention needed for its most favorable development. Some of the most essential points are given by Prof. L. C. Corbett in Circu- lar No. 90 of the Bureau of Plant Industry, as follows: PREPARATION OP LAND. Since the potato is a deep-rooted crop and forms its tubers beneath the soil, it standa to reason that it requires a much deeper seed bed than will be necessary for cereal crops. In fact, the preparation for potatoes should be as deep and as thorough as for sugar beets, whether in the irrigated or in the humid region. If a preparatory crop, such as alfalfa or clover, is to be turned under for potatoes, it is advisable to plow this crop under in the fall and to compact the soil sufficiently to make it a good retainer for water, but not so smooth that it will blow. Before planting in the spring the land should be made fine to a depth sufficient to admit of planting and cultivation. If unusually dry the land should be irrigated before the crop is planted. If the normal precipitation has occurred during the winter and spring months, the crop may be planted without irrigation. It is not advisable, however, to plant the crop in dry, hot earth and to immediately irrigate it. Irrigation should precede rather than follow [Cir. 109] 10 CIRCULAR NO. 109, BUREAU OP PLANT INDUSTRY. the planting. If the crop does not grow rapidly after planting, irrigation should be provided from time to time as the appearance of the crop and the condition of the soil would indicate ; a dark-green or blackish color shows a lack of moisture on the part of the plants, while light-green or yellowish tints indicate the presence of too much moisture. The plant should be kept growing at the maximum rate from the time it appears above the ground until it has completed its season's work, and cultivation supplemented by irrigation must be relied upon to keep the plant working. METHOD OF IRRIGATION. The water for irrigating potatoes is best applied in every other furrow, the furrows being narrow and deep and the water so applied that the ground will not be saturated above the point where the tubers are formed. This will induce the formation of a deep instead of a superficial root system. In order to accomplish this the rows must be sufficiently wide apart to admit of throwing up broad high ridges, with narrow deep furrows between, in which the water can be led in a small stream for a long period rather than by means of a large stream flowing only for a short time. The successive irrigations should be carried on hi alternate rows; the second irrigation should be in the rows not used by the first, and the third in the rows used during the first. Culti- vation should follow irrigation as quickly as the condition of the soil will permit, but as soon as the tubers have made then growth, usually about September 1, water should be withheld, so that the soil will dry and the crop ripen in proper condition for harvesting. [Cir. 109] MORPHOLOGY OF COTTON BRANCHES. 1 By O. F. Cook, Bionomist in Charge of Crop Acclimatization and Adaptation Investigation*. INTRODUCTION. The cotton plant has two distinct kinds of branches, differing in position, structure, and function. The upright "limbs," or vege- tative branches, behave like the main stalk of the plant and do not produce flowers or bolls. The floral buds appear on horizontal fruit- ing branches. The specialized habits of growth are of interest from the general standpoint of morphology, as well as in their applications to cultural and breeding problems. The practical considerations have been- treated in several publications of the Bureau of Plant Industry. 2 POSITIONS AND HOMOLOGIES OF BUDS. The positional relations of the two types of branches have seemed to throw the clearest light upon their structural homologies. It is easier to determine the positions of the buds from which the branches develop than to understand the relations of the mature branches. In addition to the bud that serves to continue the growth of the shoot, each internode of the cotton plant produces two other buds, one in the axil of the leaf and another to the right or left of the axil. Some stalks are right handed and others left handed with respect to the position of the extra-axillary buds and the branches produced by these buds. The axillary buds usually remain dormant, but may be developed into vegetative branches when the conditions are favorable to luxu- riant growth of the plants. The fruiting branch es arise from the extra- axillary buds of the main stalk and the vegetative branches. The floral buds are also developed in the extra-axillary position on the fruiting branches. Thus the floral buds of the fruiting branches have been considered homologous with the extra-axillary buds that give rise to the fruiting branches. The axillary buds of the fruiting branches are able to produce vegetative branches, like the axillary 1 Issued Jan. 4, 1913. i Weevil-resisting adaptations of the cotton plant, Bulletin 88. A study of diversity in Egyptian cotton, Bulletin 156. Dimorphic branches in tropical crop plants: Cotton, coffee, cacao, the Central Amer- ican rubber tree, and the banana, Bulletin 198. Arrangement of parts in the cotton plant, Bulletin 222. Dimorphic leaves of cotton and allied plants in relation to heredity, Bulletin 221. The branching habits of Egyptian cotton, Bulletin 249. [Clr. 109] 11 12 CIECULAR NO. 109, BUREAU OF PLANT INDUSTRY. buds of the main stalk. The tunctional specialization, of the branches oi the cotton plant is much less complete than in coffee and cacao, where one form of branches is unable to regenerate the other form. A SYMPODIAL THEORY OF THE FRUITING BRANCHES. Another interpretation, advanced by Mr. H. M. Leake, of Cawn- pore, India, makes the two kinds of branches appear much more fundamentally different. According to Leake the vegetative branches are monopodial and the fruiting branches sympodial. This means that a vegetative branch is looked upon as a single con- tinuous shoot, while a fruiting branch is supposed to be composed of a succession of independent shoots, each represented by a single internode and terminated by a flower. Thus, the floral buds of the joints of the fruiting branches would correspond to the terminal buds of the vegetative branches or of the main stalk. 1 The structure of the fruiting branches and the nature of the dif- ferences between the two kinds of branches are matters of special interest, because Leake has reported Mendelian reactions in crosses between varieties with different habits of branching. Varieties with many vegetative branches on the main stalk are described as mono- podial, while the varieties with only a few vegetative branches are called sympodial, and the two characters are supposed to reappear in definite proportions of the hybrid progenies. These results have been accepted by Bateson and used as one of the chief illustrations of the practical importance of the Mendelian methods of breeding hybrid varieties. 2 INFLUENCE OF ENVIRONMENT ON HABITS OF BRANCHING. The same characters that have appeared to Mendelize in India have shown in the United States a wide range of environmental modification. Varieties that would be considered sympodial under some conditions become altogether monopodial under other condi- tions. When the conditions keep the plants small, only a few of the vegetative branches are produced or none at all; but when the con- ditions favor luxuriant growth the fruiting branches may be replaced or transformed into vegetative branches. Even under the same con- ditions in other respects it is possible to control the development of the branches by different methods of thinning. Plants that are thinned early, so as to stand far apart during the earlier stages of growth, are likely to show a much greater development of the vege- tative branches than plants that are left closer together. In some varieties of cotton the number of vegetative branches may be in- i Leake, H. M. Studies in Indian cotton. Journal of Genetics, v. 1, p. 205, 1911. 2 Bateson, W. Genetics. Popular Science Monthly, v. 79, p. 319, 1911. [Cir. 109] MORPHOLOGY OF COTTON BRANCHES. 13 creased by the growth of more of the axillary buds into branches, but in most cases the additional vegetative branches are produced from extra-axillary buds that under other conditions would have developed into fruiting branches. The nature of the differences between the two kinds of branches must be understood before either the Mendelian reactions or the environmental transformations can be appreciated. Though there is nothing inherently improbable in the idea that the same character may show both kinds of reactions, such cases would not be expected under the Mendelian theory of heredity as a process of alternative transmission of "unit characters." It would not be expected that a character which behaved as a pure recessive in a Mendelian experi- ment could be called forth by a change of external conditions. Yet this possibility seems to be indicated by the variations that have been observed in the brandling habits of cotton. RELATIONS OF FLORAL BUDS TO STIPULES. Though a casual examination of the cotton plant may appear to support the theory that the fruiting branches are sympodial, there are several facts that seem to forbid the acceptance of this view. The relations of the floral buds to the adjacent parts of the fruiting branches do not warrant the conclusion that the floral buds of the fruiting branches correspond to the terminal buds of the vegetative branches or of the main stalk. If the floral buds of the horizontal branches were homologous with the terminal buds of the upright shoots, they should ave the same symmetrical position in relation to the stipules. But instead of standing midway between the stipules the pedicel of the floral bud is always nearer to one of the stipules than to the other. The leaves of the main stalk and vegetative branches have equal stipules, but on the fruiting branches the stipules are generally unequal. The inequality is especially pronounced in Egyptian cotton, where one of the stipules is often more than twice as wide as the other. In all cases the more enlarged stipule subtends the floral bud and frequently shows a slight attachment to the base of the pedicel. A STIPULAR RIM ON FRUITING BRANCHES. Another peculiarity of the fruiting branches, shown especially in the Egyptian cotton, is a narrow rim, or collar, that connects the bases of the stipules, so that the base of the leaf may be said to encircle the branch. This is not true of the leaves of the main stalk, where the stipules become much more widely separated, with no indication of a connecting rim. These differences may be ascribed to the fact that the thickening of the joints of the fruiting branches is not symmetrical [Cir. 109] 14 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. in relation to the base of the leaf. The faet that no stipular rim appears on the main stalk or vegetative branches doubtless results from the fact that the principal thickening of the vegetative shoots takes place in the segment of the stalk that is more directly between the stipules, whereas on the fruiting branches the first thickening is chiefly on the side that has the enlarged stipule. POSITIONS OF FLORAL BUDS. The chief reason for the sympodial theory of the fruiting branches of cotton is doubtless to be found in the fact that the flowers and fruits appear to stand opposite to the leaf of the same node, as in other plants that have sympodial habits of growth. But closer attention to the cotton plant will make it apparent that the floral bud is not directly opposite to the base of the petiole of the leaf. When younger joints of the fruiting branches are examined, the floral buds are found closer to the axillary buds — that is, more nearly in the position of the extra-axillary buds of the main stalk and the vegetative branches. The floral bud appears to have the terminal position because the true terminal bud remains relatively undeveloped until the growth of the floral bud is well advanced. The fruiting branches might be said to grow a joint at a time by successive enlargements of basal internodes of a small terminal shoot. The accelerated development of the floral bud leaves the terminal bud at one side with the axillary bud. But the apparently terminal position of the floral bud is lost when the base of the next joint thickens to its full size. This pushes the pedicel farther away from the original insertion next to the axil- lary bud and also brings it into a more erect position at an angle with the branch. When the terminal bud is aborted, as often happens, the pedicel of the last flower or boll remains in fine with the last internode of the branch. 1 The idea of sympodial branching might be strengthened by cases where the pedicel is abnormally short and thick or where the devel- opment of the next internode occurs too late to push the pedicel out of line. A still closer approximation to the sympodial condition would be reached if an aborted terminal bud of a fruiting branch were replaced by the development of an axillary bud. This probably occurs in some cases, though examples have not been observed thus far. The axillary buds are usually aborted with the terminal buds. 1 The relative rates of growth of the floral buds and the adjacent terminal buds or young shoots will probably be found to differ in the various species and varieties of cotton, like other characters. It was noticed by Mr. G. N. Collins, of the Bureau of Plant Industry, in a variety of Sea Island cotton growing at Tuxtla, Mexico , in January , 1907, that all of the involucres had one of the bracts with a deep channel or reentrant angle on the side next to the terminal shoot. It has also been observed by Mr. Rowland M. Meade, of the Bureau of Plant Industry, that the bract on the side toward the terminal shoot is usually smaller than the other bracts of the same involucre. See "Arrangement of parts in the cotton plant,' Bulletin 222, Bureau of Plant Industry, U. S. Department of Agriculture, 1911. ICir. 109) MORPHOLOGY OF COTTON BRANCHES. 15 and this is especially likely to occur on the shortened fruiting branches of small plants or at the end of the season when growth has nearly ceased. In connection with the bractlike leaves of abnormally shortened fruiting branches both the axillary and the terminal buds are sometimes completely suppressed, as in the axils of the bracts of normal involucres. TRANSFORMATION OF FRUITING BRANCHES INTO VEGETATIVE BRANCHES. Even on normal fruiting branches the pedicels of the floral buds are not turned aside far enough to bring the successive joints of the branches entirely into line, which accounts for the characteristic zig- zag form of the fruiting branches, but when the floral buds are aborted at a very early stage the branches are able to grow straight, like the vegetative branches. Such transformed branches may show the scars of aborted floral buds on a few of the lower joints, while the upper joints are without scars and are as straight and upright as normal vegetative branches. This is one of the ways in which fruiting branches are transformed into vegetative branches, but such cases of transformation during the growth of the branch are seldom found. The transformations usually occur at earlier stages of growth, before the formation of any floral buds, even on the basal joints. On nor- mal fruiting branches the basal internode is longer than the others, whereas vegetative branches often have short internodes at the base; but some branches are intermediate in this respect, as well as in other characters. Individual plants with branches of intermediate form have been found in unacclimatized stocks of Kekchi and other imported types of cotton. Though behaving as vegetative branches in other re- spects, an abortive floral bud appears at each node throughout the season. On some plants all the buds are shed while still very minute, while on other plants the buds reach more advanced stages of devel- opment. The closer the approach of the plants to normal fertility the more zigzag the branches become. The occurrence of s,uch transformations and in such various degrees does not indicate that the two forms of branches are so fundamentally different as the sympodial theory would imply. SUCCESSIVE GROWTH OF INTERNODES OF VEGETATIVE SHOOTS. That the zigzag form of the fruiting branches may be due to the accelerated growth of the floral buds is also indicated by a similar tendency in rapidly developing terminal shoots of main stalks or vegetative branches, especially on large luxuriant plants. Here the joints also appear somewhat zigzag at first, apparently because the [Cir. 109] 16 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. young leaf and the fruiting branch of each internode have a period of very rapid growth when they take nearly upright positions and throw the delicate terminal shoot out of the perpendicular. The petiole of a young leaf often becomes thicker, for a time, than the next joint of the stalk. Subsequent thickening of the joints pushes the leaves and branches over to the side, so that the zigzag form of the young terminal shoots is gradually lost and the mature stalk becomes straight and upright. In cluster varieties the mature stalks may still show the zigzag form, which is doubtless connected with the fact that such varieties have an accelerated development of • short, thick fruiting branches. Thus, if the zigzag form were to be accepted as a sufficient indication, the main stalk might be consid- ered sympodial as well as the fruiting branches, but there is no occa- sion for such a theory, in view of the fact that the mam stalk has no floral buds or branches opposed to the leaves. CONCLUSIONS. There is no apparent necessity to consider the normal development of the cotton plant as resulting in any truly sympodial structure. The pseudosympodial form of the fruiting branches arises from the fact that a flower, instead of a branch, is developed from the extra- axillary bud, and from the further peculiarity that the fruiting branches have a stronger tendency to develop one joint at a time. But notwithstanding these differences the two kinds of branches may be considered as homologous in other respects. The floral buds have an extra-axillary position on the internodes of the fruiting branches, corresponding to the extra- axillary position of the buds that produce the fruiting branches on the mam stalk or the vegeta- tive limbs. Transformations of branches from the fruiting to the vegetative form afford additional evidence that the two types of branches are not so fundamentally different as the sympodial theory Would imply. Changes from one form of branches to the other are readily induced by differences of external conditions. [Cir. 109] THE WILTING COEFFICIENT FOR PLANTS IN ALKALI SOILS. 1 By Thomas H. Kearney, 2 Physiologist in Charge of Alkali and Drought Resistant Plant Investigations. INTRODUCTION. In a previous publication of this bureau 3 the wilting coefficient was defined as " the moisture content of the soil (expressed as a percentage of the dry weight) at the tune when the leaves of the plant growing in that soil first undergo a permanent reduction in their moisture content as the result of a deficiency in the soil-moisture supply. By a permanent reduction is meant a condition from which the leaves can not recover in an approximately saturated atmosphere without the addition of water to the soil." It follows that when the moisture content of a given soil falls below the percentage representing the wilting coefficient, the plants are unable to continue their growth. In the present publication, therefore, the soil water in excess of the wilting coefficient will be referred to as "moisture available for growth." The term "alkali" is here used in the popular sense to designate those alkali and alkali-earth metals (Na, K, Ca, Mg) which are most frequently present in excess in the soils of arid regions, together with the acid radicles (CI, S0 4 , C0 3 , HC0 3 ) with which these bases are generally associated hi alkali soils. The experiment described in the following pages was designed to ascertain whether an alkali content of the soil not sufficiently high to cause evident injury to the plants would have the effect of raising the wilting coefficient; in other words, whether the plants under such conditions would reach a permanently wilted condition before they had exhausted the soil moisture to a point corresponding to the wilting coefficient as calculated from the moisture equivalent for the type of soil used. It was assumed that if this proved to be the case it would be possible to determine the minimum concentration at which alkali of a given composition becomes the limiting factor for plant growth from the inability of the plants to reduce the soil moisture to the calculated wilting coefficient. For this purpose it was decided to grow seedling plants of wheat in a series of soil mixtures having a gradually increasing salt con- tent. Each soil was to contain at the outset the same amount of water available for growth — i. e., the same percentage of moisture .above the calculated wilting coefficient — and no additional water 1 Issued Jan. 4, 1913. 2 Dr. L. J. Briggs, Physicist in Charge of Biophysical Investigations, Bureau of Plant Industry, cooper- ated by having the moisture equivalents determined and the soil extracts prepared in his laboratory. The chemical analyses of the extracts were made by Mr. J. F. Breazeale, of the Bureau of Chemistry. 3 Briggs, L. J., and Shantz, H. L. "The wilting coefficient for different plants and its indirect determi- nation," U. S. Department of Agriculture, Bureau of Plant Industry, Bulletin 230, 1912. 71357— Cir. 109—13 2 17 18 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. was to be supplied. It was believed that this method of experi- ment would eliminate all varying factors except the salt content of the soil solution. In order to determine whether the plants reduced the moisture content to the wilting coefficient, it was necessary to provide against direct evaporation from the soil during the course of the experiment. This provision also served to reduce to a mini- mum variations in the concentration of the solution in different portions of the soil mass. STOCK SOILS USED. Two natural soils from North Platte, Nebr., one of which had a much higher salt content than the other, were employed. Each was moistened sufficiently to make it easy to handle and was then thoroughly mixed. That a high degree of uniformity was thus obtained is shown by the close agreement of the determinations of electrical resistance, moisture content, and moisture equivalent * made on different portions of each stock soil. The two stock soils had a mean moisture equivalent of 25.5 and 24.1 per cent, respectively, indicating wilting coefficients of 13.8 and 13.1 per cent. They gave, when saturated, a mean electrical resistance of 205 and 38 ohms, respectively. 2 The total water- soluble material was 0.17 and 1.03, respectively, in percentages of the dry weight of the soil. The chemical composition of the alkali salts in each of these stock soils is given in Table I, which follows. The data were obtained by analyses of extracts prepared in the ordinary manner for alkali determinations — agitation for 30 minutes with 500 c. c. of water per 100 grams of soil. Table I. — Chemical composition of the extracts from soils used in the experiment, in percentages of the total solids in the extracts. Soil Total soluble (in percentage of dry weight of soil). Percentages of the total soluble solids. No. Ca. Mg. K. Na. CI. SO,. C0 3 . HCO3. 78 0.17 1.03 12.2 1.6 5.1 11.5 6.9 14.3 29.2 2.0 8.8 8.7 44.2 25.7 79 8.4 The composition of the soluble matter was markedly different in these two soils, that which had a low salt content (No. 78) having 1 The moisture equivalent has been shown by Briggs and McLane (Bulletin 45, Bureau of Soils, 1907) to be an accurate indicator of the moisture-holding capacity of the soil and to be more readily determinable than the moisture capacity itself. As defined by Briggs and Shantz (Bulletin 230, Bureau of Plant Indus- try, p. 56), it is "the percentage of water which it [the soil] can retain in opposition to a centrifugal force 1,000 times that of gravity." The method followed in determining moisture equivalents is described on pp. 56 and 57 of Bulletin 230. From the moisture equivalent the wilting coefficient can be calculated by means of a formula given on p. 58 of the same publication. 2 In the standard container (capacity about 50 c. c. ) used in determinations of the salt content of the soil by means of the electrolytic bridge. The apparatus is described by Dr. L. J. Briggs in Bulletin 15, Bureau of Soils, 1899, pp. 32 to 35. See also "The electrical bridge for the determination of soluble salts in soils," by R. O. E. Davis and H. Bryan, Bulletin 61, Bureau of Soils, 1910, wherein tables are given for temperature correction and for computing from the resistance at 60° F. the percentage of salts present. [Cir. 109] THE WILTING COEFFICIENT FOli PLANTS IN ALKALI SOILS. 19 been absolutely, as well as relatively, richer in calcium and magne- sium, but both absolutely and relatively very much poorer in sodium, in chlorids, and especially in sulphates. Soil No. 78 is also relatively richer, although absolutely poorer, in potassium and bicarbonates. In order to insure as nearly as possible the same percentage of water available for growth in all soil mixtures used in the experiment, suf- ficient water was added to each stock soil to give them practically the same "available" moisture content. The total water contents at- tained were 24.4 per cent in soil Xo. 78 and 23.9 per cent in soil No. 79, which gave available-water contents of 10.6 and 10.8, respectively. PREPARING THE SOIL MIXTURES. It was decided to use in the experiment stock soil No. 78 and 14 mixtures of the two stock soils. A graduated salt content in these mixtures was obtained by varying the proportions of the two stock soils. Precautions were taken to reduce evaporation to a minimum during the mixing and subsequent handling of the soils. Determina- tions of the electrical resistance (in ohms, at 60° F.) and of the total water-soluble material, moisture equivalent, and moisture content (in percentages of dry weight of soil) were made upon each mixture as prepared for planting. These values, together with the initial con- centration of the soil solution (calculated from the total water-soluble material and the initial water content) , the wilting coefficient (calcu- lated from the moisture equivalent), and the percentage content of water available for growth (by subtraction of the wilting coefficient from the percentage of total water present) are given for each of these mixtures in Table II. Table II. — Electrical resistance, total soluble material, concentration of solution, mois- ture equivalent, uniting coefficient, moisture content, and moisture available for growth in the soil mixtures used (means of all determinations) . Mixing pro- portions of stock soils. Soil- mix- ture Electri- cal re- sistance. Total soluble material. Initial concen- tration of solu- tion. 1 Moisture equiva- lent. Wilting coeffi- cient (calcu- lated). Moisture content. Moisture available for 78 79 No. growth. Ohms. Per cent. 20 1 200 0.183 0.75 25.5 13.8 24.4 10.6 19 1 2 105 .222 .94 25.3 13.7 23.6 10.1 18 2 3 136 .236 1.00 25.5 13.8 23.6 9.8 17 3 4 122 .305 1.24 24.5 13.3 23.6 10.3 16 4 5 111 .336 1.42 24.5 13.3 23.7 10.4 15 5 6 99 .365 1.54 24.4 13.3 23.7 10.4 14 6 7 89 .405 1.71 25.0 13.6 23.7 10.1 13 7 8 81 .418 1.76 25.5 13.8 23.7 9.9 12 8 9 76 .488 2.06 25.2 13.7 23.7 10.0 11 9 10 72 2.409 (?) 25.0 13.6 23.6 10.0 10 10 11 65 .509 2.14 23.7 12.9 23.8 10.9 9 11 12 62 .027 2.63 24.9 13.5 23.8 10.3 8 12 13 59 .675 2.85 24.5 13.3 23.7 10.4 7 13 14 55 .740 3.15 24.1 13.1 23.5 10.4 6 14 15 50 .759 3.17 23.8 12.9 23.3 10.4 1 Since the percentage of moisture corresponding to the wilting coefficient for the 15 soils averaged 56 per cent of the percentage representing the initial moisture content and since there was little change in the total salt content of the soils (as shown by determinations of electrical resistance) during the period of the experiment, it follows that the concentration of the solution in each soil had very nearly doubled when the wilting coefficient was reached. 2 There was evidently an error here, either in taking the sample or in the determination. [Cir. 109] 20 CIRCULAR NO. 109, BUREAU OP PLANT INDUSTRY. The figures for moisture equivalent in Table II show some irregu- larities, doubtless attributable to errors in the method of determina- tion. Such errors could be eliminated only by making a larger number of determinations for each soil. The contents of water available for growth in the whole series of mixtures ranged from 9.8 to 10.9 per cent, but these differences were hardly of sufficient magnitude to affect the results of the experiment. Chemical analyses of extracts from each of these soil mixtures showed, for most components, an increase from mixture to mixture corresponding to the increase of total soluble matter. A note- worthy exception was HC0 3 , of which practically the same quan- tity was found in all. The mixtures having the lowest total salt content had the highest content of calcium and magnesium, as would be expected from the fact that larger amounts of these bases existed in stock soil No. 78 than in stock soil No. 79. PLANTS USED AND CONDITIONS OF GROWTH. Kubanka wheat (Grain Investigations No. 1440) was used, the stock having been the same as that employed by Messrs. Briggs and Shantz in their investigations of the wilting coefficient. The seeds were placed in moist blotters on April 3; three days later they were in proper condition for planting. While no special effort was made to select seedlings of the same size, none were planted winch did not have the pluinule and the rootlets extruded. All seedlings planted appeared to be perfectly healthy and had no roots much longer than 1 inch. The seedlings were planted in straight- walled, cylindrical drinking glasses, each holding about 250 grams of soil. Five seeds were planted in each glass and there were six glasses of each soil mixture, making a total of 30 seedlings planted in each soil mixture used in the experiment. In planting, most of the soil required was placed in the glass and was shaken down by tapping the bottom of the glass on the table. The seedlings, taken directly from the moist blotters, were laid on the loose surface of the soil; the glass was then filled evenly to the brim with the remaining soil, so as to completely cover the seedlings, and the soil was pressed down firmly, first around the edges and then in the center. The surface was then sealed over with- a melted mixture of 80 per cent of paraffin and 20 per cent of petrolatum, as described in a previous publication of this bureau. 1 In order to equalize as far as possible the temperature of the soil in the glasses and to prevent condensation, which would result in an unequal distribution of the water and salts in different portions i Bulletin 230, pp. 10-14, [Cir. 109] THE WILTTNO COEFFICIENT FOR PLANTS IN ALKALI SOILS. 21 of the soil mass, the glasses were kept in a partitioned metal tank, through which a stream of water was kept flowing during the whole period of the experiment. 1 A light tent of bolting cloth was stretched over the tank during hours of bright sunlight, in order to prevent, if possible, the heating and melting of the wax seals. 2 EMERGENCE OF THE PLANTS. A record was kept of the rate at which the plants in each glass appeared above the surface of the soil, this record having been based on daily observations during the first 12 days (with the exception of the eighth day after planting) and a final observation on the four- teenth day, after which no more plants emerged. In mixture No. 15 no plants appeared. Table III shows the total number of plants (out of the 30 inserted) which finally appeared above the seal and the number of days from the date of planting to that when the last plant emerged in each soil mixture. Table III .—Rate oj emergence of the plants in the different soils. Soil-mixture No. Number of plants iliai emerged. Number of days until emergence ceased. Soil-mixture No. Number of plants that emerged. Number of days until emergence ceased. 1 30 30 30 30 30 30 30 30 4 4 4 4 4 4 4 4 9 30 30 24 15 23 10 6 2 10 7 3 11 10 4 12 14 5 13 10 6 14 14 7 15 8 The behavior of the plants in the several glasses of the more con- centrated soils (Nos. 11 to 14) was decidedly puzzling. In mixture No. 11 all plants emerged in five of the glasses and none in the sixth. In mixture No. 12 all plants emerged in three of the glasses and none in the other three. In mixture No. 13 all plants emerged in three of the glasses, all but one of the plants in two others, and none in the sixth. In mixture No. 14 all plants emerged in two of the glasses and none in the other four. No explanation could be found for these irregularities. Electrolytic bridge readings at the close of the experi- ment showed that they could not be attributed to differences in salt content of the soil as between the glasses in which growth was made and those in which no plants emerged. 1 This method is described and illustrated in Bulletin 230, Bureau of Plant Industry, p. 13. 2 In spite of these precautions, owing to the rather high temperatures that prevailed during the period of this experiment , some softening and buckling took place in several of the glasses, which made it neces- sary to break the seals in order to release plants caught beneath them. The seals were repaired so quickly that no appreciable loss of water by direct evaporation could take place. This could have been obviated by using for the seals a composition having a higher melting point. rrtr mm 22 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. Nevertheless, the figures given in Table III show clearly a retarding effect of the more strongly saline soil mixtures. Observations at more frequent intervals than every 24 hours might have shown some retar- dation in mixtures less concentrated than No. 9, although it was noted that emergence was at least as prompt in No. 5 as in Nos. 1 to 4. CHARACTER OF GROWTH. On April 13, one week after planting, the longest leaf in each glass was measured and the mean of these values for the six glasses of each soil was taken as expressing the growth made in that soil while there was still abundant "available" moisture. Table IV shows the values thus obtained. Table IV. — Mean length of the longest leaf in each soil one week after planting. Soil-mixture No. Mean electrical resistance. Mean length of the long- est leaf. Soil-mixture No. Mean electrical resistance. Mean length of the long- est leaf. 1 Ohms. 200 165 136 122 111 99 89 81 Millimeters. 153.5 156.0 138.0 134.0 128.0 121.0 113.0 96.7 9 Ohms. 76 72 65 62 59 55 50 Millimeters. 81.0 2 10 66.0 3 11 42.9 4 12 29.0 5 13 21.0 6... 14 29.0 7 8 These data show a much more marked correlation between the rate of growth of the plants and the salt content of the respective soils than the figures relating to rate of emergence given in Table III. In soils Nos. 1 to 8 the seedlings made a good, healthy growth in all the glasses. In Nos. 9 to 1 1 their growth was good in the main, but the plants were smaller. In Nos. 12 to 14 the growth was irregular in the glasses in which any plants emerged, some plants having been small and sickly, while others had made a fair growth and appeared fairly normal. For some reason the average growth was better in No. 13 than in No. 12. DETERMINATION OF THE WILTING COEFFICIENT. As soon as the plants appeared to be in a wilted condition the soils were collected from the glasses for moisture determination. Table V gives the final water content of the soil in each glass in which sufficient growth was made to reduce the water content to anywhere near the wilting coefficient, as well as the mean of these determinations for each soil mixture. For comparison, the mean wilting coefficient is given for each mixture, as calculated from the moisture equivalents at the outset of the experiment. [Cir. 109] THE WILTING COEFFICIENT FOR PLANTS IN ALKALI SOILS. 23 Table V. — Water contents of the soils when plants first appeared wilted, with calculated wilting coefficients of the corresponding mixtures. Soil-mix- ture No. Glass No. Water content when plants first appeared wilted. For each glass. Mean for each soil mixture. 13.4 13.3 13.2 12. S i 13. 3 13.4 15.4 13.7 13.5 14.6 13.9 13.8 14.2 14.0 14.1 14.8 14.5 14.5 14.7 14.8 13.8 14.1 14.9 15.1 14.7 14.0 14.4 14.1 15.0 14.1 14.0 13.2 14.0 13. 6 15.2 14.5 13.2 14.1 14.3 14.6 Wilting coefficient (calcu- lated from the moisture equiva- lents). 14.4 14.1 13.8 13.7 13. S 13.3 13.3 13.3 Soil-mix- ture No. Glass No. 10. 11. 12. 13. I I Water content when plants first appeared wilted. For each glass. 13.5 13.8 13.4 13.9 13.6 13.2 13.0 12.8 13.4 13.4 13.3 13.5 15.2 14.5 14.1 14.4 14.0 13.6 13.6 14.0 14.1 13.4 14.1 14.6 14.0 14.2 13.8 13.9 14.7 15.2 13.4 12.8 15.5 13.3 13.3 13.5 14.8 Mean for each soil mixture. Wilting coefficient (calcu- lated from the moisture equiva- lents). 13.6 13.2 14.3 14.0 14.1 13.6 13.8 13.7 13.6 12.9 13.5 13.3 13.1 The figures in Table V show the mean water content at the time of apparent wilting to have been higher in most of the soil mixtures than the calculated wilting coefficient. 1 Nevertheless, even in the most strongly saline mixture (No. 14) in which any emergence took place, the plants in at least one of the glasses were able to reduce the moisture content to a percentage agreeing, within the limits of experimental error, with the calculated wilting coefficient. On the other hand, in glasses where the plants remained very small and sickly this was not possible. Thus, in one of the glasses of mixture No. 13, in which the plants began to die at about the date i Probably because the high temperatures that prevailed toward the close of the experiment made it rather difficult to distinguish between temporary and permanent wilting. More accurate determina- tions of the permanent wilting point could doubtless have been made if the plants, as soon as they ap- peared to be wilted, had been transferred to a saturated atmosphere in order to determine whether they could recover their turgidity. [Cir. 109] 24 CIRCULAR NO. 109, BUREAU OP PLANT INDUSTRY. when the wilting coefficient had been reached in the least saline mix- tures, the soil-moisture content (determined when it became evident that the plants would make no further growth) was found to be 22.8 per cent. Since the initial water content of this soil was 23.7 per cent and the initial moisture available for growth was 10.4 per cent (Table II), the loss of water by transpiration in this glass amounted to only 3.8 per cent of the total and 8.6 per cent of the available moisture present at the outset. While the moisture content of all the soils was finally reduced in at least one of the glasses to the wilting coefficient, tins happened much more rapidly in the soils winch had a high electrical resistance (low salt content) than in those which had a low electrical resistance (high salt content). There was, on the whole, a remarkably close correla- tion between the salt content of the soil and the length of time required for the plants to reach a wilted condition. The relation is expressed hi Table VI. Table VI. — Time required to exhaust in each soil mixture the water available for growth. Number of glasses in which wilting occurred. Soil mixture No. Mean electrical resist- ance. Mean number of days from date of plant ing until wilting began. Number of glasses in which wilting occurred. 1 200 165 • 136 122 111 99 89 18.0 18.0 19.3 22.0 25.0 23.5 24.3 6 6 6 6 6 6 6 2 3 4 5 6 7 Soil mixture No. Mean electrical resist- ance. Mean number of days from date of planting until wilting began. 8 9 81 76 72 65 62 59 55 27.0 27.6 31.5 33.4 36.0 38.7 43.5 10 11 12 13 14 CONCLUSIONS. The results of this experiment make possible the following conclu- sions : (1) The presence of an excess of soluble salts in a soil did not affect the ability of young wheat plants to reduce ultimately the water con- tent of that soil to the wilting coefficient, unless the quantity of salts was sufficient to induce marked pathological symptoms in the plants. (2) The time required for the exhaustion of the water available for growth increased steadily with increasing concentration of the soil solution (Table VI). (3) The amount of growth made, so. long as the moisture content of the soil remained well above the wilting coefficient, was deter- mined by the concentration of the soil solution (Table IV). (4) The presence of alkali increased the water requirement of the plants ; in other words, increased the quantity of water transpired in LCir. 109] THE WILTING COEFFICIENT FOR PLANTS IN ALKALI SOILS. 25 producing a unit weight of dry matter. The conditions of the experi- ment here described did not allow of accurate determinations of water requirement, but this conclusion seems unavoidable in view of the following facts: (a) Some of the plants in each soil mixture were able to reduce the moisture content to the wilting coefficient. Hence, practically the same total quantity of water was finally lost by transpiration from each soil, (b) The plants in the more strongly saline soils were much smaller when the wilting coefficient was reached than were those in the soils having a lesser salt content. Experiments are now in progress to measure the effects of different quantities of alkali upon the water requirement of plants. [Cir. 109] INTERPRETING THE VARIATION OF PLAT YIELDS. 1 By F. D. Farrell, Agronomist, Office of Western Irrigation Agriculture. FACTORS OF ERROR IN FIELD EXPERIMENTS. All results obtained in field experiments are subject to errors the extent of winch can not always be accurately determined by physical measurements. These errors may be due to a large number of causes, such as incorrect weighing of crop products, faulty determinations of plat areas, variations in the quantities of products recovered and wasted, unobserved variations in field treatment, etc. These are termed experimental errors, and the influence of some of them can be determined and controlled. Other important causes are individual plant variations, soil irregularities due to natural conditions or to nonuniform previous treatment, uneven distribution of soil moisture, temperature variations, etc. The influence of these factors can sel- dom be accurately determined or controlled. Frequently the total effect of all these causes is so great as seriously to influence the conclu- sions drawn from observed results, and if it is not taken into con- sideration the results may be badly misinterpreted. Where a large number of observations are made of results obtained under condi- tions winch are uniform, except as they are affected by accidental error, the extent of that error can be calculated by a mathematical formula, and a part of the calculation results in what is known as the "probable error." EXPERIMENTAL DETERMINATION OF THE PROBABLE ERROR. FIELD TESTS IN NEBRASKA AND MONTANA. In order to determine as nearly as possible to what extent these factors would influence the crop yields of plats to be used in the field experiments under irrigation at the Scottsbluff (Nebr.) and Huntley (Mont.) experiment farms, certain of the fields to be used were planted to uniform crops and given uniform treatment. In 1911 field K, at Scottsbluff, containing 66 quarter-acre plats, and field K, at Huntley, containing 68 quarter-acre plats, were planted to oats. At Huntley in the same year fields B II and B III, which are adjacent, and which contain 46 quarter-acre plats, were planted to sugar beets. In 1912 fields B II and B III, at Huntley, were planted to alfalfa. Ten adjacent quarter-acre plats were planted to alfalfa in 1912 in 1 Issued Jan. 4, 1913. [Cir. 109] 27 28 CIRCULAR NO. 109, BUREAU OP PLANT 'INDUSTRY. field A II at Huntley. In each case the plats were harvested sep- arately, and the yields obtained form the basis of the material pre- sented in this paper. The results obtained with 66 plats of oats on field K at Scottsbluff in 1911 will serve as an illustration of the method pursued. These plats received uniform treatment throughout the season, so that without a consideration of accidental errors it would be expected that the plat yields would be equal ; or, in other words, it would be expected that the yield of any plat taken at random would fairly represent the yield of any other plat in the field. Such an expectation is, of course, never realized. It was found in the case of the oat plats at Scotts- bluff that the yields varied from 372 pounds to 212 pounds — an extreme range of 160 pounds per plat. This variation is equivalent to 20 bushels per acre, or 55.7 per cent of the mean yield. Where a difference of 20, or even 10 or 5, bushels per acre is obtained from two plats receiving different treatments the difference is commonly con- sidered significant and attributable to the treatments. But it is obvious that a difference of 5 bushels per acre would not have been significant on field K in 1911 if the oat plats had received different treatments. It is commonly found that approximately one-half the results observed in a series will be greater than the mean of all the results and that approximately one-half the results will be less than the mean, and also that the number of results differing from the mean will decrease as the magnitude of the difference increases. For example, there are 29 plats in field K which yielded within 24 pounds of the mean yield, while only 10 plats differed from the mean yield by more than 48 pounds. Because of this tendency for the majority of the results to lie relatively close to the mean, the mean is taken as a basis upon which to calculate the probable error. In a "perfect" series " The probable error is such that, talcing any single result at random, the chances are even for or against that result differing from the average by the amount of the probable error. In other words, half the results should differ from the mean by less than the probable error, the other half by more." 1 The probable error is calculated in the following manner: The mean of all the results is determined. This mean is subtracted from each result to obtain the difference, each difference is squared, and the sum of these squares, disregarding the sign, is found. Then the following formula is employed: The probable error of a single result= P fi7 .r /The sum of the squares of the differences. yThe number of results less 1. i Wood and Stratton. Journal of Agricultural Science (Cambridge), v. 3, pt. 4, p. 417. December, 1910. [Cir. 109] INTERPRETING THE VARIATION OF PLAT YIELDS. 29 The 66 plats at Scottsbluff are not a sufficient number to form a "perfect" series, where the variations are so great, but they afford an opportunity of calculating the probable error with sufficient accuracy for practical purposes. The mean yield of the 66 plats is 287 pounds and the probable error of any single result is found to be ±23.9 pounds, or 8.3 per cent of the mean yield. This is equivalent to 3 bushels per acre. According to the above definition of probable, error, 33 plats should yield within 23.9 pounds of the mean, and the yield of the other 33 plats should vary from the mean by more than 23.9 pounds. It was actually found that 29 plats varied from the mean by less than the probable error and 37 varied by more. While those numbers 29 and 37 are not equal, they are as close as can be expected in a series of only 66 observations and close, enough for practical purposes. The specific application of these determinations to field experiments is this: In a series of plats planted to a single crop and given uniform treatment marked variations may occur and from these variations the probable error may be determined. If, then, in the same series of plats later planted to a single crop and given different treatments, the variations in yield do not exceed the probable error as previously determined, these variations can not properly be attributed to the effects of the treatments and they should not be considered as sig- nificant. In other words, the probable error may be employed as a measure of the confidence which can safely be placed in experimental results. The more a variation, supposedly due to a treatment, ex- ceeds the probable error the greater the. confidence which can properly be placed in the supposition that the treatment is responsible for the variation. If a variation is greater than twice the probable error, its significanee is very materially increased, antl if it is so large as to exceed three, four, or five times the probable error, its significance approaches a practical certainty. In the series of plats at Scottsbluff only ten plat yields Varied from the mean by more than twice the probable, error, and only four varied by more than three times the probable error. There were no vari- ations from the mean equal to four times the probable error. In other words, to have obtained a variation equal to four times the probable error it would have been necessary to introduce, a variation in the treatment. If under this different treatment a variation in yield greater than four times the probable, error were obtained, it would be a practical certainty that the variation was due to the treatment. But any variation less than this would be subject to the suspicion that it might be due to accidental errors, such as soil irregu- larity, etc., rather than to treatment, and the suspicion would increase as the size of the variation decreased. [Cir. 109] 30 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. Table I contains the probable error determinations made on five groups of plats, one group at Scotts bluff, Nebr., and four at Huntley, Mont., together with some other data associated with the probable error in each case. Explanations of the various items are given after the table under the respective numbers of the items. Table I. — Probable error determinations made on five groups of field plats at the Scotts- bluff and Huntley experiment farms . Items. 1. Mean yield (groupsA,B,D,andEln pounds: group C in tons per acre) „ /Number of plats yielding above the mean \ Number of plats yielding below the mean 3. Extreme range of plat yields, expressed in percent- age of mean yield of each group 4. Mean deviation of plat yields, expressed in per- centage of mean yield of each group 5. Probable error of asingle result expressed in terms of yield 6. Probable error, expressed in percentage of mean yield of each group: a Of any single result 6 Of the average of 10 results c Of the average of 20 results d Of the average of 30 results e Of the average of 40 results /Of the average of 46 results a Of the average of 66 results ft Of the average of 68 results 7. Number of plat yields differing from mean: a By less than the probable error — Actual Theoretical 6 By more than the probable error- Actual Theoretical c By less than twice the probable error d By more than twice the probable error « By more than three times the probable error. / By more than four times the probable error. . Groups. A. 66 plats oats, Scotts- blufffleld K, 1911. 287 35 31 55.7 9.8 ±23.9 8.3 2.6 1.9 1.5 1.3 1.2 1.0 29 33 37 33 56 10 4 B. 68 plats oats, Huntley field K, 1911. 554 37 31 106.0 22.5 ±105.95 19.1 6.0 4.3 3.5 3.0 2.8 2.34 2.31 26 34 42 34 63 5 C 46 plats beets, Huntley fields B II and Bill, 1911. D. 46 plats alfalfa, Huntley fields B II and B III, 1912. 11.71 29 17 67.9 12.9 ± 1.29 ±11.0 3.5 2.4 2.0 1.7 1.6 25 23 21 23 38 8 4 356 23 23 52.5 12.5 ±38.18 ±10.7 3.4 2.4 1.9 1.7 1.6 19 23 27 23 38 8 E. 10 plats alfalfa, Huntley field A II, 1912. 572 5 5 51.5 9.6 ±49.27 ± 8.6 ±2.7 6 5 4 ."> 7 3 1 (1) The mean yield of each group of plats is expressed in pounds per plat, except in the case of group C, where, for convenience, it is expressed as tons per acre. (2) This item is given to show how closely the plat yields in the different groups agree with the assumption that approximately one-half the results will exceed the mean. It is shown that in groups D and E the division is exactly equal; in A it is very close, and in B and C it is rather noticeably unequal. It should be stated that in group C, 7 of the 46 plats were spring plowed and the others fall plowed in prepara- tion for beets. To this extent the treatment of the plats in group C was not uniform. The mean yield of the 7 spring-plowed plats was 0.83 ton per acre less than the mean yield of all 46 plats. The discrepancy introduces an error into the calculations, but it has only a very slight effect on the probable error. (3) The extreme range in the yields obtained in each group is stated as a percentage of the mean yield of each group so as to facilitate comparisons among the groups. The extreme range in the plat yields at Scottsbluff was from 372 to 212 pounds per [Cir. 109] INTERPRETING THE VARIATION OF PLAT YIELDS. 31 plat, a total variation of 160 pounds, or 55.7 per cent of the mean yield. It will be noted that a high extreme range is closely associated with a high probable error. (4) This item includes the mean of the deviations from the mean yield of each group expressed as a percentage of the mean yield. It will be noted that this is closely associated with and slightly greater than the probable error of a single result in each case. (5) The probable error of a single result is first given in terms of yield. The prob- able error of ±23.9 pounds in the case of group A means that if the yield of any plat in the group is taken at random the chances are even that the yield of such plat will be within 23.9 pounds of the mean yield of the group. It should be noted that the figure expressing the probable error is always plus or minus. Hence, while a result may vary from the mean by less than the probable error, it may be either greater or less than the mean. (6) In order to facilitate comparisons the probable error is here stated as a percentage of the mean yield. The probable error in group A is ±23.9 pounds of oats per plat, or 8.3 per cent of the mean yield. The probable error of ±49 pounds of alfalfa per plat in group E is 8.6 per cent of the mean yield of that group. When reduced to a per- centage basis the probable error of group A and that of group E are seen to be very nearly the same. The probable error of group C and that of group D are also extremely close. This fact is of special interest, because the fields used were the same in both cases — fields B II and B III, Huntley — while the crops were different and were, of course, produced in different seasons. So far, the probable error of a single result, the yield of a single plat, has been con- sidered. When the average of several results is taken, the probable error of that average is, of course, less than the probable error of one result. In other words, the average yield of 10 plats is likely to represent the mean yield of the group more nearly than does the yield of any single plat taken at random. The average yield of any 10 plats taken at random in group A will have a probable error of ±7.59 pounds per plat, or 2.6 per cent of the mean yield, as compared with 8.3 per cent of the mean yield, the probable error of a single result. If, then, the number of results averaged be increased to 20, the probable error is still smaller — 1.9 per cent of the mean yield in group A ; and the probable error of the average grows continually smaller as the num- ber of results averaged is increased, as is shown in the table. The fact that the prob- able error of the average of several results is less than that of a single result empha- sizes the desirability of conducting field experiments in multiplicate. The probable error of an average can be obtained by dividing the probable error of a single result by the square root of the number of results averaged. (7) Under this heading are given the number of plat yields differing from the mean by more or by less than the probable error or one of its multiples. Under a and b is given the number of results which, theoretically, should differ from the mean by more or by less than the probable error, as well as the actual number found. Theo- retically, half the results should differ from the mean by less and half by more than the probable error. The table shows that the actual results are in the main reasona- bly close to the theoretical requirements. They are as close as can be expected where such small numbers of results are considered. Under d, e, and fit is seen that comparatively few plat yields differ from the mean by more than twice the probable error, still fewer by more than three times, and none by more than four times the probable error. Hence, it may be expected that on these fields accidental variations as great as four times the probable error are not likely to occur; so that when different treatments are applied and variations greater than four times the probable error are obtained, such variations can be attributed to the treatments with a high degree of certainty. [Cir. 109] 32 CIRCULAR NO. 109, BUREAU OF PLANT INDUSTRY. FIELD TESTS IN ENGLAND. Wood and Stratton, of Cambridge, England, have published some results relative to the probable error of field experiments. Their in- vestigations covered numerous observations on wheat, barley, oats, mangels, potatoes, and other crops. They observed that there was no significant difference between the probable error of one of these crops and that of any other. This is in a way supported by the close agreement found at Huntley with sugar beets and alfalfa, groups C and D, where the same field was used for loth trials. But Wood and Stratton take 5 per cent as the probable error of a single plat in carefully conducted field experiments. 1 The facts that at Huntley and Scottsbluff widely different probable errors were found for the same crop (oats) and that the probable errors determined at the two farms are considerably greater than 5 per cent indicate that to take 5 per cent as " the probable error of field experiments " is not generally justified. CONCLUSIONS. The material here presented may be used to emphasize the follow- ing points: (1) Probable error determinations are valuable as a measure of the reliability of results of field experiments and of the confidence which may properly be placed in conclusions drawn from such results. (2) The value of experiments would be greatly increased if the probable error applicable to the field were determined in advance; and where the experiments contain several plats receiving the same treatment it is desirable to determine each year the probable error of the results obtained on such plats, as in this way the effect of seasonal factors on the probable error may be estimated. (3) In conducting field experiments all possible precautions should be taken in order to minimize accidental errors. (4) It is always desirable to conduct tests in multiplicate, so as to be able to compare averages rather than single results. (5) In interpreting and publishing experimental results the prob- able error should be given consideration; otherwise, insignificant differences may be unduly emphasized. 1 " The probable error of field experiments is investigated by two independent methods, and found to be about 5 per cent of the crop."— Journal of Agricultural Science (Cambridge), v. 3, pt. 4, p. 440. De- cember, 1910. [Cir. 109] ADDITIONAL COPIES of this publication -ii- may be procured from the Superintend- ent of Documents, Government Printing Office, Washington, P. C. , at 5 cents per copy U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 110. B. T. GALLOWAY, Chief of Bureau. MISCELLANEOUS PAPERS. Grass Demonstrations in the South. Some Asiatic Actinidias DAVID FAIRCHILD Powdery Dry-Rot of the Potato W. A. ORTON Preparation of Land for Egyptian Cotton in the Salt River Valley, Arizona . E. W. HUDSON Agriculture on the Truckee-Carson Project: Vegetables for the Home Garden . . F. B HEADLEY and VINCENT FULKERSON Fungous Staining of Cotton Fibers ALBERT MANN The Jack Bean and the Sword Bean C. V. PIPER Fiber from Different Pickings of Egyptian Cotton .... T. H. KEARNEY LIBRARY NE W YORK EN Issued January 18, 1913. WASHINGTON: GOVERNMENT PRINTING OFFICE. 1913. [Cir. 110] 2 BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant chief of Bureau, William A.Taylor. Editor, J. E. Rockwell. Chief Clerk, James E. Jones. GRASS DEMONSTRATIONS IN THE SOUTH. The accompanying correspondence, dealing with the subject of demonstrations in grass culture, is submitted as showing the interest in this kind of work in South Carolina. It is published by direction of the Honorable Secretary, who for years has advocated the pro- duction of grass and legumes as a step toward greater diversification of crops and the increase of animal husbandry in the Southern States. MEMORANDUM FOR THE SECRETARY. Dear Mr. Secretary: You may be interested in the efforts being made to encourage the production of grass as a feature of stock raising in South Carolina. Last spring Mr. W. W. Long, of our Farmers' Cooperative Demonstration Work, inaugurated a series of demon- strations in portions of the Piedmont region to show the possibilities of grass and hay production. He took up the work with 100 different farmers, inducing each of them to make an acre demonstration with grass. The predominant industry of this section is cotton raising and not a great deal of interest is taken in live stock. Each farmer put out an acre of grass under the direction of Mr. Long. The land was thoroughly plowed, a ton of lime to the acre was applied, and, in addition, 400 pounds of fertilizer used. The fertilizer consisted of 200 pounds of acid phosphate and 200 pounds of ground bone. The acid phosphate cost $12 a ton and the ground bone $29 a ton, which would make the cost of the 400 pounds of fertilizer $4.10. The lime cost about $5 a ton. The farmers furnished all the work, the lime, and the fertilizer. The grass seed was furnished from cooperative funds. The most successful mixture for hay in this section of the South is found to be one made of orchard grass, tall meadow oat-grass, Italian rye-grass, and red clover. A half bushel each of orchard grass, tall meadow oat-grass, and Italian rye-grass, and 10 pounds of red-clover seed were sown to the acre. This very heavy seeding was believed to be necessary in order to insure a good stand. We have been using this mixture for some time on the Arlington Experimental Farm with good success, cutting as high as 2J and 3 tons of hay to the acre. The work in South Carolina has been very satisfactory, practically all of the 100 farmers having a fine crop of hay this season. From all indications, some of these plats will cut 2h to 4 tons per acre. So much interest has been aroused in the matter that farmers are now preparing to put out 2 additional acres, making 3 acres in all. They have found by this demonstration that they can grow enough hay 1 Issued Jan. IS, 1913. [Cir. 110] 3 4 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. to take care of the two or three head of horses used in their general farm work. The success with the hay crop has encouraged farmers, furthermore, to look into the live-stock proposition to the end of introducing greater diversification and devoting less acreage to cotton. Very respectfully, B. T. Galloway, Chief of Bureau. April 28, 1912. memorandum for the secretary. Dear Mr. Secretary: You will probably recall that' last spring we sent you a memorandum concerning some important demonstra- tion work in South Carolina by Mr. Long, who is connected with this Bureau under Mr. Knapp. Mr. Long started out with the plan of demonstrating to the farmers of South Carolina the practicability of growing grass. About 100 good farmers were selected and each agreed to put out an acre of grass under Mr. Long's direction. In the memorandum which I forwarded you the success of the work at that time was indicated. As a result of further work a great deal of interest has been aroused in the subject. Mr. Long informs me that 300 demonstration plats have been put out this fall. Recently Hon. A. F. Lever made a trip over the State in company with Mr. Long, and I inclose herewith a copy of a letter winch Mr. Lever has forwarded; also a copy of a letter which he wrote to Prof. English, of Clemson College, both of which are self-explanatory. Very respectfully, November 25, 1912. B. T. Galloway, Chief of Bureau. [Inclosure.] Prof. B. T. Galloway, Washington, D. C. Dear Dr. Galloway: I am inclosing herewith a copy of a letter which I have written to-day to Prof. English, of Clemson College, which explains itself. I have expressed to him what I desire you to know as my feeling in regard to this grass- demonstration work now being conducted in this State. I do not believe that I shall have any hesitation in saying that the Department is undertaking no greater work than this is, and I sincerely trust that the funds set aside for this work for this season will be sufficient to enable it to develop as rapidly as possible. I desire to talk with you about it when I come on to Washington. I regret that you could not be with us on the trip, as 1 know that you would have enjoyed it. With personal regards, I am, very truly, A. F. Lever, Member of Congress from 'South Carolina. Lexington, S. C, November 22, 1912. [Inclosure.] Prof. W. L. English, Clemson College, S. C. My Dear Prof. English: You will recall seeing Mr. Long, Mr. Dorrick, and me starting on our trip to look at the grass demonstrations being conducted by the Depart- ment of Agriculture in 20 of the counties of this State. I have become so much inter- ested in the work and feel that it is of so much importance to the future of the State that I felt that I should call your attention to it that you in turn might bring the work to the notice of those in authority at Clemson. [Cir. 110] GRASS DEMONSTRATIONS IN THE SOUTH. 5 The demonstration plats in the upper part of Richland County have been growing for one year, and not only do they strike a layman, such as I, as having been entirely successful, but the demonstrators themselves are enthusiastic over the results ob- tained and are proving it by the fact that they are increasing their acreage, and their neighbors are following their example. The plat of Mr. Sam C. Cathcart, a few miles above Winnsboro, was particularly successful, for that gentleman told me that he had gathered 3 tons of first-class hay from a little less than an acre of ground and that he valued it at about $33 per ton, that being the price of good timothy hay at Winnsboro. Mr. Cathcart is not only a large farmer, but runs a considerable-sized dairy, and he tells me that in this grass work of the Department he sees the beginning of the solution of the agricultural problems of this State as far as forage crops are concerned and as far as soil building goes. From our Fairfield trip we came back into the Dutch Fork of Lexington County and saw there the plat of Dr. J. L. Shuler, one of the most substantial citizens 'of the State; and not only could we see with our own eyes that it had been entirely suc- cessful, but Dr. Shuler assured us that it was successful beyond his expectations, and as a result he has doubled his acreage this year. Mr. James W. Shealy, who is in charge of this section of the county, informs me that his 3 acres of experimental work for this past season has been increased to 17 for the coming season, which, in my mind, is evidence not only of the success of the demonstrations, but of the interest of the people in it, who are beginning to realize that they must grow more in connection with cotton. We came back into the territory covered by our mutual friend, Judge Derrick, and saw two of his demonstrations, one on the farm of Mr. G. W. Caughman and one on that of Mr. D. J. Caughman. The former has his demonstra- tion plat upon about as poor a piece of red Spanish oak land as you ever saw, and notwithstanding this he got from it 2 tons of fine hay. Mr. D. J. Caughman has his plat on better land, and yet not the best type of land by any means, and he tells me that he cut his grass three times and gathered 3 tons to the acre. He is now grazing his hogs upon it, and the clover is 6 or 8 inches high and practically green. The cured hay is as fine as I ever saw. What I have seen on this trip convinced me that my own idea of the feasibility of growing grasses in this country, formed some 10 years ago, was entirely correct. We can grow grasses without any doubt, and it is up to the Department of Agriculture and Clemson College workers to lock arms and demonstrate that fact to the farmers of the State. To my mind there can be no line of work undertaken that will mean so much ultimately to the wealth of the State as to induce our farmers to grow their own forage crops; and this, as you know, is unequaled as a soil builder and con- servator. I think every student of the agricultural future of this State is agreed upon the proposition that we must make ourselves, to as large an extent as possible, a live-stock State. We must grow cattle; we must have some substantial crop with which to reenforce our cotton crop; we must have some way of reducing the enor- mous fertilizer bills of the State. We must find some means of producing enough beef and dairy products in this State to save us the enormous drain because of our deficiency hi this respect; and, to my mind, this grass work, if we can make it suc- cessful, and I am sure it can be made successful with the proper efforts behind it, will bring about the very condition of affairs which, I think you will agree, is so necessary to the agriculture of the State. I have taken the liberty of writing you at this length because of my very deep interest in this work and because of the impression I formed of you personally of your great desire to be of the greatest benefit to the people of the State. With personal regards, I am, very truly, A. F. Lever. Lexinoton, S. C, November 22, 1912. [Cir. 110] SOME ASIATIC ACTINIDIAS. 1 By David Fairchild. Agricultural Explorer in Charge of Foreign Seed and Plant Introduction. ACTINIDIA ARGUTA. At least five species of Actinidia are now growing in this country, and they deserve much more consideration than has been given them. There is no finer climbing shrub for porches in this latitude than Actinidia arguta Miq. Its foliage seems to be practically free from diseases, is of a beautiful dark-green color with reddish midribs, and for situations where a mass of uncontrolled irregular foliage is wanted and when a trellis can be provided, it is unusually successful. It is a very vigorous grower and will cover a trellis 20 feet long and 10 feet high in two or three years. Several years ago the writer had the pleasure of tasting a few fruits picked from a vine of this species which covered over a hundred feet of trellis on the house of Mr. Charles N. Parker, of Marblehead, Mass. This vine had fruited more or less regularly since it was 9 years old, and it was at that time 20 years of age. The flavor of the fruits was very sweet and pleasant, reminding one of figs. They were about the size of damson plums, with very thin skins and filled with extremely small seeds. A woodcut of the fruit of this species has been published in Bailey's Cyclopedia of American Horticulture. The value of this plant as a fruiting vine seems to have been little emphasized in America. The long time required for it to come into bearing and its dioecious nature, which has no doubt resulted in many males being planted, has probably disheartened Americans from growing it more extensively. From Asia, however, there have come various reports about its usefulness and productiveness which make it seem probable that there are better fruiting strains there than we have in America. In northern Chosen (Korea) Mr. J. D>. Hubbard, of the Oriental Consolidated Mining Co., of Unsa, informed the writer in 1 909 that this species was known there as the tara, or wild fig, and that it climbed 30 feet high and fruited profusely. ACTINIDIA CALLOSA. The species Actinidia callosa Lindl. is allied to A. arguta and probably has not been grown outside of arboretums and botanic gardens in America. 'Issued Jan. 18, 1913. [Cir. 110] 7 8 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. ACTINIDIA POLYGAMA. In the Arnold Arboretum, vines of Actinidia polygama Miq. have been growing successfully for years, and from one point of view, at least, it is one of the most interesting plants in the arboretum. Its leaves and twigs are so relished by cats that a wire cage has been constructed about it, and evidently they still molest it, for wherever the twigs or leaves come near enough to the wire netting they are scratched and mutilated by the cats and cats' hairs are sticking to the wires, which are evidences of efforts to get at the plants. Mr. Jackson Dawson's observations indicate that the Boston cats learned that this was good to eat a few months after it was first introduced. ACTINIDIA KALOMIKTA. Actinidia Icalomikta Ruprecht, which has also proved hardy in Boston, although so far as known it has not fruited, is reported to fruit well in the mountains near Merkoechofka, Siberia. The fruits are dried by the Russian settlers and put aside for winter use in the making of confectionery and to put in their bread. Mr. Frank N. Meyer has described a variety of this species from Tungying, China, which is shorter and shrubbier than the ordinary one. He found the same species indigenous in the mountains of Okyansky in eastern Siberia. In the mountains of northern Chosen (Korea), where he saw it in August, 1906, he reported it to be a scant bearer. ACTINIDIA CHINENSIS. The most striking species of this interesting genus yet brought into America is Actinidia cJiinensis Planch., known in China as the yang- taw, and it is of very recent introduction. The attention of English horticulturists was first called to it by Mr. Robert Fortune, who found it when traveling hi behalf of the Royal Horticultural Society. Mr. E. H. Wilson was the first to send seeds of it to Veitch & Sons, in London, from his first expedition to western China. As early as 1900 seeds were sent to the Office of Foreign Seed and Plant Introduc- tion from Ichang, but they failed to grow. In 1904, however, the office received through the initiative of Consul General L. S. Wilcox, of Hankow, a shipment of plants which had been carefully packed at Chungking by Mr. E. H. Wilson, at that time engaged in an explora- tion of Szechwan Province. The shipment arrived in excellent con- dition, and as it is from these plants that the distributions through the Southern States have been made, sufficient historical interest attaches to it to warrant publishing Mr. Wilcox's letter in full. [Cir. 110] SOME ASIATIC ACTINIDIAS. 9 [No. 115.] Hon. Francis B. Loomis, Assistant Secretary of State, Washington, D. C. Sir: I have the honor to inform you that last fall I obtained a sample of fruit called by the Chinese "yang-taw." On investigation I learned that the original plants were brought by Mr. Wilson, a botanist of Kew Gardens, London, from near the borders of Yunnan in the foothills in the southern part of the province of Szechwan (a climate similar to southern California). He sent some of the plants to England, where they endeavored to introduce them, but found the climate unsuitable, being too cold, except those that were planted in the Kew Gardens. The latter have proved a success. The botanical name given them at Kew Gardens is Actinidia chinensis. The fruit is about the size of a hen's egg,. has a thin, leathery, hairy skin covering it, and is full of meat; seeds very similar to a gooseberry or fig. It is sometimes called the hill goose- berry. In bloom it has a beautiful flower, and in my opinion it belongs to the same family as the passion flower. When the fruits are picked and left for a few days until soft they are very fine eating. They have the flavor of the gooseberry, fig, and citron. They make delicious jam, pies, and sauce. I asked Capt. Lovett, the Chinese Imperial Maritime Customs' Harbormaster here, if it was possible to procure a few plants, as I would like to send them to the United States Department of Agriculture. We supposed they had come from Ichang and would be like currant shoots. He wrote to the party that sent the fruit (Mr. Goodhart, of Ichang) who said he would try. After giving up all idea of receiving them, a box came two days ago, weighing three or four hundred pounds, with the information that they had been secured at Chungking (1.000 miles up river), from plants formerly obtained on the borders of Yunnan by Mr. Wilson, under whose advice they have been packed in moss and sand, warranted to keep for months. I felt I had a white elephant on my hands; the bill for them has not yet been presented. I have inquired of several southerners and none of them are acquainted with the fruit in the South, and it cer- tainly would be a valuable fruit to introduce there. I think it worth the cost and trouble of sending it. I will forward it to Shanghai and request Consul General Goodnow to have it transferred to a Japanese steamer to Consul Harris, of Nagasaki, and have requested him to put it on a transport for San Francisco, in care of Mr. William A. Cooper, U. S. Despatch Agent, to hold it pending instructions from the State Department as to where he is to ship it. I will also write Mr. Cooper to inform the State Department of its arrival. I have packed in the box two bottles of the fruit that I put up in alcohol last fall in order that the Agricultural Department may get some idea of the two varieties that I received. The long variety seems to be the finer flavored. As the plants are carefully packed and strongly boxed, I trust that they may arrive in good condition and that the fruit may be more valuable for family use than the navel orange. At least I trust it will not be the case of a "mountain bringing forth a mouse." I have the honor to be, sir, your obedient servant, L. S. Wilcox, Consul General. Hankow. China, March 21, 1904. At the Plant Introduction Field Station at Chico, Cal., these plants have flowered (PI. I) and have been propagated. Since 1904 the Office of Foreign Seed and Plant Introduction has sent out 1 ,340 young plants of this remarkable climber, and one of these flowered in 1910 at Durham, N. C. Unfortunately, however, the speeimens which flowered at Chico turned out to be males, and therefore the plants 72780°— Cir. 110—13—2 10 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. propagated and distributed are valuable only as ornamentals. Our subsequent introductions of seedling plants have not as yet given any indication of flowering. Doubtless there are females among them. Repeated efforts to get authentic female plants of good varieties from China have been unsuccessful, owing to the difficulty of getting living plants properly packed and shipped from the interior. In the Gar- deners' Chronicle for July 31, 1909, is an account of the first flowering of this species in Europe. The flowers borne in 1909 at Nice, France, were also all males. It is therefore too early to predict anything as to the probable behavior and value of the species from the fruit- culture point of view. Reports have reached us that a specimen of this species has fruited at Veitch & Sons' nurseries, near London, but no details have yet been published, so far as the writer is aware, regarding the quality of this fruit borne in England. These facts are much to be regretted, since from Mr. Wilson's descriptions the fruit must be excellent. In his report of 1898 he describes the plant as "fruiting abundantly, bearing fruits 1 to 2\ inches long and 1 to If inches across (PI. II). Epicarp membranous, russet brown, more or less clothed with villous hairs. Flesh green, of most excellent flavor, to my palate akin to that of the common goose- berry but tempered with a flavor peculiarly its own." 1 Dr. Samuel Cochran, of Hope Hospital, Hwaiyuan, Anhwei, China, in a letter dated September 26, 1911, writes : We find them delicious; they have something of the tart, rich flavor of the goose- berry or strawberry. Not that the flavor is the same, but it has the same attractiveness, being entirely free from mawkishness or insipidity. They make excellent preserves. The flavor scarcely needs improvement. The fruit eats well raw after the skin is re- moved, needing about as much sugar as strawberries. Rev. William F. Beaman, of Kiatingfu, near Yachow, has reported orally to the writer that the flavor of this fruit varies remarkably, some tasting like a pineapple and others like a strawberry, confirm- ing in this respect the experience of Mr. Leigh Hunt respecting the Korean species (probably Actinidia arguta) . According to Mr. Hunt, who spent many years in northern Chosen (Korea), one could find on the same vine fruits of a flavor to suit everyone's taste. Others have written enthusiastically of this fruit, and photographs and alcoholic specimens show it to be one worthy of consideration. The behavior of the plant solely as a climber warrants its wide distri- bution, however, and it has already been taken up by at least one American nursery firm. This species is not so hardy as the others mentioned, being killed to the ground by severe freezes, but the rapidity of its growth in the spring and the interesting character 1 Wilson, E. H. In Seeds and plants imported during the period from January 1 to March 31, "1908: Inventory No. 14, U. S. Department of Agriculture, Bureau of Plant Industry, Bulletin 137, p. 14, No. 21781, 1909. [Cir. 110] Cir. 1 1 0, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate I. TO r* 03 a-? ■5 . o o °p 32 m o3 a a oj O . Ss ■" o ^ P 5 o o -I CD < I- i o z < >• UJ X 1- u z 11 i- - TO Oft ai-a^j a — u ssa ,^ St o a° s - .a -o .^> 'OBI' in 03 > .5 Xl "3 t* o 5 03 -S 03 S-ci-a •HpO > *- fc-l 2 ftbC 2 Cir. 1 10, Bureau of Plant Industry, U. 5. Dept. of Agriculture. Plate II a o w I ft) — Soo A J! < -3° > M o % ui "3 - < 3S g o 0> B O SOME ASIATIC ACTIXIDIAS. 11 of its rapidly growing shoots will make it a desirable plant for trellises and porches far north of the region where it can pass the winters unin- jured (fig. 1). The remarkable plushlike texture of the leaves, their unusual dark-green color, the bright-pink hairiness of the young shoots, and the regular spacing of the leaves on the stem all contrib- Fig: 1.— The yang-law on a wall trellis. Shoots produced before the middle of Julv bv a vine of Ac/inidia chinensis Planch, which was killed to the ground by a temperature of —17° F. the previous winter. Before autumn the trellis was almost concealed by the foliage. The trellis is on the north side of the house, and the spaces of the trellis are 10 inches square. Photographed at "In the Woods," Chevy Chase, Md., July ute to make this climber especially suited to locations where broad masses of green foliage are wanted. An old wall or outhouse, a broad porch or pergola, where the plant is not confined, would be a suitable place for this tit tractive vine. The Chico specimens have flowered profusely. The male flowers, which are borne in masses, are single white blossoms as large as a wild rose and with a [Cir. 110] 12 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. great quantity of yellow stamens (fig. 2). The petals fade to a pink as they wither. The flowers have a delicate but peculiar fragrance. Considering the wide range of climate to which the different spe- cies of this genus of Asiatic plants is adapted, from the climate of California to that of Maine, the thought has occurred to the writer Fig. 2.— Male flowors of the yang-taw. The flowers are pure white when they open but turn pink as they fade. The stamens are bright yellow. Natural-size photograph taken of flowers borne by tlir original introduction of the species (Actinidia chinensis Planch.) into America (S. P. I. No. 1 L629) at the Plant Inl reduction Field Station, Chico, Cal., by Dr. Walter Van Fleet, April 18, 1910. that the genus ought to be a promising one for the hybridizer to work with. Whether the pollen of male plants of Actinidia chinensis in California can be kept long enough to pollinate the female flowers of A. arguta in Massachusetts can be easily determined, and. if not, the cultivation of the different species side by side in regions where both will flower is a possibility. [Cir. 110] POWDERY DRY-ROT OF THE POTATO. 1 By W. A. Orton, Pathologist in Charge of Cotton and Truck Disease and Sugar-Plant Investigations. INTRODUCTION. In recent years a new potato disease, which has been named the "powdery dry-rot/' has come to the front. It has caused heavy losses in several Western States from Minnesota to Washington and is a special menace to those irrigated districts where the potato is one of the main money crops and where the product must be shipped many hundreds of miles to reach a market. Several instances have recently occurred where carloads of potatoes were shipped from the Northwest to Texas points and to Chicago. Leaving their point of origin in apparent good order they arrived at their destination badly decayed, were rejected by the purchasers, and had to be con- signed to the dump. The cause of this rapid deterioration was the powdery dry-rot. Such experiences are exceedingly harmful to the reputation of a new potato district. Buyers will not erect ware- houses or provide shipping facilities for a permanent trade, nor will they purchase for distant shipment save at the producers' risk. It is therefore imperative that the growers take every possible means to prevent the spread of this disease. DESCRIPTION OF POWDERY DRY-ROT. This disease is an external dry-rot. It may start at any point on the outside of the tuber or gain entrance at the stem end. It starts most readily in wounded potatoes, but may spread to uninjured ones. The spots are wrinkled, discolored, and somewhat sunken, externally darker brown than the normal epidermis, internally sepia brown, with a dark, discolored layer next the sound flesh. In the later stages the decayed portions become dry and powdery, with internal cavities filled with fungous mycelium. The cause of powdery dry-rot is a newly described fungus, Fusa- rium tricJiothecioides Wollenw. 2 1 Issued Jan. 18, 1913. J Jamieson, C. O., and Wollenweber, H. W. An external dry-rot of potato tubers caused by Fusarium trichothecioides Wollenw. Journal, Washington Academy of Sciences, v. 2, no. 6, p. 14(>-152, Mar. 19, 1912. [Clr. no] 13 14 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. The disease is still too new and our experience too limited to permit positive statements relative to means of control, but the following advice is based on what is known of the life history of the causal fungus. POSSIBLE MEANS OF CONTROL. Clean seed. — The powdery dry-rot is a storage trouble and appears not to begin work till after the harvest, yet there is evidence that land becomes infected through planting diseased seed potatoes and that this contagion is carried over until fall and communicated to the new crop. Consequently the most rigid inspection of the seed planted is advised. No potatoes with a trace of decay should go into the ground. All the seed stock should be disinfected by soaking for two hours in a solution of 1 pint of formaldehyde in 30 gallons of water. This will also kill the germs of potato scab. Rotation of crops. — Rotation is necessary for the permanent success of potato culture in any country, and particularly in irrigated dis- tricts. While it is possible to produce two or three successive crops on new land, it is unwise to attempt it on account of the disease factor. One potato crop in three years has been proved too short a rotation in many districts. One in five may serve the purpose in our Western States. If a field has produced potatoes affected by powdery dry-rot, it is especially desirable to rotate before planting potatoes there again. The storage problem. — Most important of all for the control of dry- rot is the method of handling the potatoes after digging and during storage, as it is probably here that the main fault lies. Well-built storage cellars are a necessity from an economic stand- point, to enable the grower to await better prices and to properly assort and pack his crop. In such cellars, if properly built, venti- lated, and wtached, the greatest immunity from decuy may be insured. These storage houses should be thoroughly cleaned at the end of the season, all old potatoes and debris carried out, and the walls and floors washed with a disinfectant like copper sulphate (blue vitriol), using a 1 per cent solution, or corrosive sublimate, using 2 ounces to 16 gallons of water. The greatest losses that have been brought to our attention have occurred in that portion of the crop which was not put in storage or shipped direct from the field to the market, but held for some weeks after having been dug and sacked. Such potatoes, stored in sacks in large piles and subjected to variable and occasionally rather high temperatures, offer most favorable conditions for the development of the dry-rot fungus. [Cir. 110] POWDERY DRY-ROT OF THE POTATO. 15 It is suggested that, when it is necessary to hold potatoes for a time before shipping and it is not feasible to put them into a good, cool storage cellar, the experiment be tried of leaving them unsacked in the field in long, low piles or ricks, covered with sufficient earth to protect them from sun and frost, and that they be sent from these piles to market with as little intervening delay as practicable. It is perhaps hardly necessary to warn growers that the dry-rot parasite attacks most readily bruised or wounded potatoes and that care in digging and handling, to leave them with nature's protecting skin intact, is a great insurance against loss. [CIr. 110) PREPARATION OF LAND FOR EGYPTIAN COTTON IN THE SALT RIVER VALLEY, ARIZONA. 1 By E.W. Hudson, Assistant in Arboriculture, Crop Physiology and Breeding Investigations. INTRODUCTION. The Department of Agriculture has from time to time during the past year sent out circular letters to the Egyptian cotton growers in the Salt River Valley, calling their attention to various phases of cotton culture and offering suggestions in handling the crop. The harvest of the Egyptian cotton crop of 1912 grown in the Salt River Valley is so nearly completed that the growers who have taken good care of their crop are confident of a yield of from 600 to 700 pounds of lint per acre. Owing to the success of the present crop, the growers are planning to increase their acreage and a number of the farmers throughout the valley are planning to plant on a large scale. From the present indications it is safe to estimate that from two to three thousand acres of Egyptian cotton will be planted in the valley in 1913. SELECTION OF LAND. Since it is very important that the land be prepared as early as possible in the winter, it is well for those who are planning to plant to give some thought at this time to the selection and preparation of their fields. To secure the best crop of Egyptian cotton, it is of very great importance that the grower select uniform land with very slight grade on winch alfalfa has grown for at least three years. The amount of fall or grade in the land is of great importance to the cotton crop. To produce the best crop the land should be almost level. In many instances where alfalfa is plowed up it is advisable to irrigate in a different direction to secure a lighter grade. Land with a slight grade will require less water for the crop and will irrigate more evenly and produce a more uniform cotton. If there is much grade to the land it will be found that the fields dry out hi spots dur- ing August and September, and in order to avoid injury to the quality of the cotton it will be necessary to irrigate these spots separately, thus causing much extra work. i Issued Jan. 18, 1913. 72780°— Cir. 110—13 3 17 18 CIRCULAK NO. 110, BUREAU OF PLANT INDUSTRY. The question of the soil best adapted for cotton has been raised many times. Any land that will grow good crops of alfalfa and grain will also grow good cotton. The heavier class of soil will as a rule grow a smaller and more fruitful plant with shorter nodes, and hence more numerous fruiting branches. While some raw land will make good cotton, it has been clearly demonstrated that land previously in alfalfa will produce a higher grade of cotton and the crop can be produced more economically, for new land as a rule requires more frequent irrigations and it will be found necessary to irrigate hi spots owing to the uneven way it will take water. A great deal of the best land in the Salt River Valley is overrun with Bermuda and Johnson grasses. The badly infested area comprises much of the land that was under cultivation prior to the completion of the Roosevelt Dam. There are, conservatively speaking, fully 10,000 acres of land on the south side of the river alone that would be greatly benefited by cotton. This is the best land for cotton in the valley, since most of it is level and rich from previous crops of alfalfa. PREPARATION OF THE LAND. In preparing this land for cotton the best plan would be to plow about 2 inches deep during August, allowing the soil to dry out thoroughly. This should be followed by thorough disking and har- rowing, using a long-tooth harrow and dragging as many of the roots to the surface as possible. A spring-tooth harrow might be used to advantage hi this work. During November or December the land should again be given a shallow plowing and pulverized by disking and harrowing. At this time it would be well to plow the land both ways with an orchard cultivator or similar tool with long teeth. This work will bring a great many of the roots to the surface, and if hi sufficient quantity they should be raked up and burned or hauled off the field. The land may then lie fallow until the latter part of February, when borders should be thrown up about 2 rods apart. Just before planting time, which is between March 10 and April 1, the land should be flooded and then disked and harrowed until in perfect tilth. At this time, if the soil is a very heavy clay, in some instances it may be advisable to throw up beds from 4 to 4^ feet wide and 8 inches high, with the idea of dragging off fully half of this height before planting. A drag can be easily constructed of 2 by 4 inch or 2 by 6 inch scantling that will take two or possibly three beds at a time. It should be weighted down until it drags off enough of the surface clods to get down to the moist soil. If a lighter soil is used it will not be necessary to throw up beds. It is advisable to bed some classes of heavy soil because it may be necessary to irrigate in order to germinate the seed, [Cir. 110] PREPARATION OF LAND FOR EGYPTIAN COTTON. 19 whereas a lighter soil may be pulverized sufficiently after a thorough irrigation to hold enough moisture to germinate the seed without further irrigation. Only as much land should be irrigated at one time as can be prepared and planted before becoming too dry to germinate the seed. Seed must always be planted in moist soil. The seed may be drilled in with a cotton planter, which will plant either one or two rows. This may be either a one or two horse machine. If a single- row walking planter is used it may be necessary to mark out the rows on the bed before planting in order to keep the planter on the bed. PREPARING BERMUDA AND JOHNSON GRASS LANDS. While it may cost the grower from $8 to $10 an acre to put Bermuda and Johnson grass land in shape for cotton, by intensive cultivation during the first season the grass can be kept down, although it may be found necessary to chop it out in the rows at the time the cotton is being thinned. Bermuda grass alone is not so hard to eradicate as Johnson grass or a mixture of Johnson and Bermuda grasses. It is possible by shallow plowing during November or December, followed by thorough disking and harrowing, to put Bermuda-grass land in shape for cotton. If the land is kept thoroughly disked and harrowed during the winter the freezing will greatly aid in killing the roots. Two or three weeks before planting the land should be plowed from 4 to 6 inches deep and thoroughly pulverized. If regular culti- vation be kept up during the early part of the growing season or until the cotton plants become large enough to shade the ground, the Bermuda grass will not have a chance to establish itself enough to become a nuisance. By such culture it is believed to be possible for the growers to eradicate the grass entirely within two or three years, growing a remunerative crop on the land. PREPARING ALFALFA LAND. In preparing for cotton alfalfa land which is not overrun with Ber- muda or Johnson grass the same general plan could be followed. However, it will not be necessary to do as much disking and harrowing or cross plowing as in the case of land infested with Bermuda and Johnson grass. If alfalfa land is plowed 2 inches deep and turned up to the sun until thoroughly dried and then later in the season plowed 4 to 6 inches deep there will be very little trouble caused by alfalfa during the following season. Alfalfa land may be prepared any time prior to the planting season, but the best results will be obtained if the land is plowed first in October or November, followed by a second plowing in January. [Cir. 110] 20 CIECULAE NO. 110, BUREAU OF PLANT INDUSTRY. PREPARING COTTON OR GRAIN LAND. In preparing cotton land for again planting to cotton a stalk cutter should be used to chop the stems into small pieces; then the land should be plowed, disked, and harrowed until in perfect tilth, when it may be left until planting time. In the absence of a stalk cutter the plants can be dragged down with a heavy drag after a hard freeze. A great many of the stalks will be pulled out, and those remaining in the ground must be dug up with a mattock. This operation is inexpensive, costing only $1 or $1.25 per acre. After all the plants are pulled out of the ground the field should be raked crosswise with a hayrake and the stalks put into windrows, where they can be easily burned. Land previously in cotton or grain if irrigated before plowing can be put in perfect condition with one plowing. Land previously in alfalfa should be plowed twice. Last year one farmer plowed his land and leveled it veiy poorly, and instead of disking several times after the irrigation just before planting the seed made small furrows and planted the seed with the idea of pulverizing the land when cul- tivating. This piece of cotton had to be hoed twice and cultivated several times more than a near-by field which was double disked and harrowed until it was in perfect condition before the seed was planted. A farmer planting cotton will make a very great mistake if he does not thoroughly prepare his land before planting the seed. It depends upon the kind of soil and the condition it is in whether a double plowing is necessary to put the land in good tilth or whether this can be done with a single plowing and double disking and double harrowing. No amount of labor within reason expended during the whiter in preparing the seed bed will be regretted, since if this is thoroughly done a great deal less work during the summer will be required to grow the crop, and the yield will be correspondingly better. [Cir. 110] AGRICULTURE ON THE TRUCKEE-CARSON PROJECT. 1 VEGETABLES FOR THE HOME GARDEN. By F. B. Headley, Superintendent, and Vincent Fulkerson, Scientific Assistant, Western Irrigation Agriculture. INTRODUCTION. The notes on vegetables presented in this circular embody the results of trials for three years on the Truckee-Carson Experiment Farm. Cultural directions have been given only in part. Most farmers have some knowledge of the growing of vegetables, and it is therefore only the special or little-known methods that are suggested here. Many of the common vegetables are so easily grown on the Truckee- Carson Project that every farmer should set aside enough land for a vegetable garden, where sufficient produce can be raised to supply the family throughout the season. Among the most easily grown vegetables are asparagus, beans, beets, carrots, cucumbers, eggplants, kohl-rabi, lettuce, muskmelons, onions, peas, peppers, potatoes, pumpkins, radishes, squashes, tomatoes, turnips, and watermelons. A well-kept vegetable garden on the farm is one means of lessening the cost of living, and during the summer it provides a large variety of foods that a farmer could not otherwise afford, and even during the winter, if proper care be taken to preserve them. Asparagus, beans, cucumbers, peas, pumpkins, squashes, beets, and tomatoes may be put up in glass jars and preserved satisfactorily. 2 Winter muskmelons, pumpkins, squashes, and many of the root crops can be kept by storing them in pits or cellars. CULTURAL DIRECTIONS. Asparagus. — This is a perennial crop and should therefore be planted in some portion of the garden where it will not interfere with the easy cultivation and growing of other crops. The best method of starting an asparagus bed is by planting 1-year-old roots, i Issued Jan. 18, 1913. 2 See Farmers' Bulletin 359, entitled "Canning vegetables in the home." [Cir. 110] 21 22 CIRCULAE NO. 110, BUREAU OF PLANT INDUSTRY. but the plants may also be grown from seed. A very fertile soil is essential and the crop should therefore be manured heavily each year. It is better not to harvest the asparagus the first two seasons after planting, for it is necessary that a strong root system be devel- oped. Commencing with the third year, harvesting may begin. Asparagus will grow in soils too alkaline for most other crops. Beans. — As beans are tender annuals, they must be planted after danger of frost is past, which on the Truckee-Carson Project is about May 15. Over 20 varieties have been tried at the experiment farm in comparative tests. As the result of these tests the following varieties are recommended for home use: Bush varieties of string beans, green Pole varieties of string beans: podded: Kentucky Wonder Pole. Early Yellow Six Weeks. Field varieties for dry shelled beans; Burpee's Stringless. Colorado Mexican. Bush varieties of string beans, wax Navy, podded: Mexican Pinto. Improved Golden Wax. Dwarf Horticultural. Davis White Wax. Beets.— Beets are of easy culture and will grow in soil containing considerable salt. The subsoil should be loose and mellow, as a hard- pan tends to cause the roots to grow short and misshapen, with many side roots. A late planting may be made between June 20 and July 15 for autumn and winter use. For fall planting a turnip-shaped variety rather than a long-shaped, slow-growing variety should be selected. Carrots. — Carrots are somewhat more difficult to grow than beets. The seeds are small and slow to germinate, so that it is important to have a mellow, well-tilled soil — one that does not "bake" or crust badly. Sometimes seeds of quick-maturing radishes are mixed with the carrot seeds. The radish seeds, being relatively large and quick germinating, break the crust and make it easy for the tender carrot seedlings to come through. The soil for the carrots should be loose and mellow, as a hardpan subsoil tends to make them grow short, misshapen, and much branched. A late planting may be made dur- ing the last half of June for autumn and winter use. Corn. — Sweet corn does not usually produce well on desert soils, but it grows well on old alfalfa land. The greatest difficulty in con- nection with the growing of sweet corn is due to the prevalence of the corn earworm, for which no effective remedy is known. It is essen- tial that early varieties be selected. Peep o' Day, Golden Bantham, and Early Minnesota are good varieties to use. Cucumbers. — As cucumbers are sensitive to frost they must be planted after danger of frost is past, unless arrangements are made [Cir. 110] AGRICULTURE ON THE TRUCKEE-CARSON PROJECT. 23 for covering and protecting them. They transplant with difficulty, and it is therefore usual to plant the seed in the hills where they are to grow. This crop is easily grown and does well on most soils of the Truckee-Carson Project. The heaviest yielding and most satisfac- tory varieties tried so far at the experiment farm are the Early Frame, Long Green, Early Russian, and White Spine. The Gherkin is a small, productive, prickly variety, useful only for pickling purposes. Kohl-rabi. — This vegetable has been on trial at the experiment farm for three years and has always given good results. The flavor of kohl-rabi is between that of a turnip and a eabbagc. It is necessary to use this vegetable while it is growing and tender, as when it is fully matured it is likely to be tough and stringy. The culture is the same as for turnips. Unharvested plants need not be destroyed in the summer when they have become too tough for use, for if they are left in the ground until fall a second growth takes place, new bulbs grow- ing out of the old ones. This growth is as sweet and tender as the spring growth. Melons. — Watermelons are easily grown and are very productive in most of the soils of the project. As the growing season is short, only the earliest maturing varieties should be planted. Those which have given best satisfaction at the experiment farm are the Kleck- ley Sweet, Kentucky Wonder, Chilean, and Rocky Ford. Muskmelons, like watermelons, are easily grown, especially on the lighter soils of the project. Winter varieties, such as the Khiva, Winter Pineapple, and Kriss Kringle, may be successfully grown. These varieties, when stored in a cool place, will keep well into the winter, so the farmer may have muskmelons on his table for Christ- mas and New Year's Day. Desirable early-maturing summer varie- ties are the Rocky Ford, Fordhook, and Extra-Early Hackensack. Both watermelons and muskmelons may be planted in the field before the danger of frost is past if boxes, guimy sacks, straw, or some other suitable means of protection are thrown over them on cold nights. Onions. — On some of the better soils of the Truckee-Carson Project onions are grown commercially and a yield of 10 to 20 or more tons per acre obtained. It would not be advisable to attempt to grow them commercially on new land. Local onion growers have found it advisable to plant the seed very early in the spring, before April 1, so as to allow the plants to become well established before the dry, hot weather begins. Good varieties to plant for the home garden are the Yellow Globe Danvers, Red Wethersfield, and Prize Taker. Peanuts. — Peanuts have been grown for several years, but satis- factory yields have not been obtained. The small Spanish peanuts matured earlier and yielded better than the Mammoth Virginia pea- nuts. Peanuts can not be recommended for general planting. [Cir. 110] 24 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. Peas. — Peas usually produce a good crop in the gardens over the project, but sometimes fail in raw or alkaline soils. The Champion of England and the Alaska were the best-yielding varieties tried at the experiment farm in 1912. The seed should be planted very early in the spring. Potatoes. — Potato growing 1 is one of the recognized agricultural industries of Nevada. Potatoes are best grown on river-bottom land, tule land, or old alfalfa land. They do not always grow well on the raw desert soils, which are deficient in humus and likely to contain harmful quantities of alkali salts. The varieties which have given best results at the experiment farm are the White Beauty, Burbank, Triumph, Mammoth Pearl, Peachblow, and Early Ohio. A variety test was conducted in 1911 by Wallace Ferguson in coop- eration with the experiment farm. Eight varieties were included in the test. The three heaviest yielding varieties were the White Beauty, Mammoth Pearl, and Rose Seedling. Pumpkins and squashes. — Pumpkins and squashes should be grown in every farmer's garden, as they are prolific and are valuable for table use. Any overproduction can be profitably fed to dairy cows or hogs. Good varieties of pumpkins for table use are the Japanese Pie, Small Sugar, and Cushaw. They are sweet and fine grained. The large varieties, such as the Mammoth King and Connecticut Field, are heavy producers, but they do not have the fine flavor and quality for cooking of those mentioned above. The most desirable summer squashes tried are the Yellow Summer Crookneck 2 anjd White Bush Scallop, and the most desirable winter varieties tried are the Warted Hubbard and Delicious. The Mam- moth Chile was the heaviest yielding variety, but its quality is not good for cooking purposes. Tomatoes. — Tomato seed should re sown about April 15 in boxes or hotbeds, so as to have good-sized plants by the time danger of frost is past. The growing season is so short that an early start is essential. Tomatoes usually produce satisfactory crops, but they are subject to a disease known as the wilt (Fusarium sp.). This disease attacks the individual plants in the tomato patch. The first indication is a wilting of the leaves of the affected plants. The wilt becomes more noticeable from day to day, until it finally causes the death of the plants. No remedy is known, but damage from the disease can be reduced by growing the plants on soil that has not recently produced tomatoes. As the wilt is infectious, all diseased plants should be pulled and burned as soon as they become affected. i For the culture of the potato, see Farmers' Bulletin 3S6, entitled " Potato culture on irrigated farms of the West;" or "Nevada potatoes," by C. A. Norcross, Nevada Bureau of Industry, Agriculture, and Irri- gation, Bulletin 6, 1912. 2 Also known as the Golden Summer Crookneck. [Cir. 110] AGRICULTURE ON T1TE TRUCKEE-CARSON PROJECT. 25 Good varieties are Early Jewel, Dwarf Champion, New Stone, New Coreless, New Globe, and Golden Qneen. After frost has killed the vines the larger green tomatoes may be picked, each wrapped in a piece of newspaper, packed in a shallow box and left to ripen by storing in a closet or other suitable place where there is no danger of freezing. By doing this, farmers may have ripe tomatoes up to Thanksgiving time. Turnips and rutabagas. — Turnips seeded in April are usable late in June or early in July, but soon become woody and bitter. Ruta- bagas are ready for use by the middle of July. For winter use ruta- bagas should be seeded in June and turnips in early July. If seeded earlier than this they become woody in the late fall. When the greater part of the growth takes place in the cool weather of autumn the quality is better and the size larger. Wonderberry and garden Imckleberry . — The fruit of the wonderberry is fairly agreeable when eaten raw, but is used chiefly in the making of pies and jams. The vines are usually heavily loaded, but the fruit is so small that it is somewhat difficult to gather. The plant is an annual, but reseeds itself, so that volunteer plants come up in the garden each year. The garden huckleberry is advertised by some seedsmen as being the same as the wonderberry, but the two plants are very different. The garden huckleberry is an upright-growing, bushy plant, while the wonderberry is decumbent. The fruit of the garden huckleberry is two or three times as large as that of the wonderberry and when raw is disagreeable to the taste. It must be cooked to be palatable. After cooking there is practically no difference in flavor between the two fruits. These two fruits do not deserve to be extensively grown, but under conditions on the Truckee-Carson Project they are suffi- ciently valuable to deserve a place in the family garden, at least until the more' desirable small fruits have come into bearing. [Cir. 110] FUNGOUS STAINING OF COTTON FIBERS. 1 By Albert Mann, Plant Morphologist, Agricultural Technology and Cotton Standardization and Grading Investigations. OCCURRENCE OF THE DEFECT. The manufacturers of certain grades of cotton fabrics are occa- sionally annoyed by the appearance of bright-red or deep purple- blue threads appearing in white cotton cloth, thereby greatly lower- ing its commercial value. The number of such threads in a square yard is extremely small, but the brilliant colors make these threads very prominent. The American Cotton Yarn Co. has sent to the Office of Agricul- tural Technology and Cotton Standardization and Grading Investi- gations two sets of manufactured samples showing these defects, with a request for information as to the cause. Inquiry makes it practically certain that the fibers are not colored in the process of manufacture. Careful microscopical examination of the colored fibers gave no indication of bacteria or of fungous growth. The colors were very resistant to alkalis of moderate strength, as well as to bleaching agents. PROBABLE DISCOVERY OF THE CAUSE. Recently a boll of cotton was obtained from Diamond Spring, Va., containing a quantity of lint that was brilliant carmine red. Exam- ination showed that this was due to the presence of a fungus, which was identified by Dr. H. W. Wollenweber, of the Office of Cotton and Truck Disease and Sugar-Plant Investigations, as belonging to the genus Fusarium. The fungus was very evident, completely investing the cotton fibers and even penetrating their internal cavities. It was in fruit, and the sickle-shaped compound spores of this genus were abundant. A Fusarium grows upon various host plants in the United States, including the cotton plant, which under alkaline conditions may have either a strong carmine-red or an intense violet-purple pigmentation. It is Fusarium metaclwoum App. and Wr. Unlike some blue and red pigments in plants, the two colors are not convertible into each i Issued Jan. 18, 1913. [Cir. 110] 27 28 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. other by the alternate action of alkaline and acid reagents, though both result in a yellow color under acid modification. This agrees so closely with the facts observed in the colored fibers of the cotton cloth that it seems probable these colored fibers are caused by growths of fungi, and probably of the species mentioned, occurring upon cotton exposed to excessive dampness in the field or picked in an unripe condition. The reason the fungus was not discovered on the manufactured fibers is probably due to the mechani- cal and chemical processes through which it passed. The samples of cloth submitted had been mercerized. The external hyphae of the fungus would probably be removed in the various processes of ginning, spinning, • and subsequent treatment. The absence of hyphae within the fibers may have been because of no penetration having taken place in the specimens examined, or possibly because of some obliterating effect of the process of mercerizing the cloth. The manufacturers are now preparing to adopt some means of eliminating these undesirable samples of cotton from the raw material. U'ir. 110] Cir. 1 10, Bureau of Plant Industry, U. S. Dept. of Agriculture. Plate III. Pods and Seeds of the Jack Bean and the Sword Bean. Central figure, pod of a jack bean (S. P. I. No. 21609) from Brazil. Right-hand figure, a pod of a sword bean (S. P. I. No. 27676) from India. Left-hand figure, pod of a sword bean (S. P. I No 27704) from China. THE JACK BEAN AND THE SWORD BEAN. 1 By C. V. Piper, Agrostologist in Charge of Forage- Crop Investigations. THE JACK BEAN. During the past few years the jaek bean (Canavali ensiformis) has attracted a good deal of attention in Texas and other Southern States. Many inquiries have been received concerning its agricul- tural value, as under ordinary conditions it grows vigorously and produces large yields of beans. DESCRIPTION. The jack bean is a bushy, semierect annual plant, growing to a height of 2 to 4 feet. Its stems are rather coarse and become woody toward the base. The rather thickish leaves have a decidedly bitter taste. The flowers are purple, at least in all varieties so far intro- duced. The first blossoms are borne near the base of the stem, so that many of the pods hang low. When mature the pods are hard and firm, 9 to 14 inches long, each containing 10 to 14 seeds. These are pure white, with a brown hilum. Ordinarily the roots are well tubercled, and the plant will withstand much drought. It is re- markably free from insects and fungous diseases and but slightly affected by root-knot. (PI. III.) HISTORY. The jack bean is a native of the West Indies and the adjacent mainland. In Jamaica, whence it first became well known, it is called the horse bean or the overlook bean. The horse bean of Europe is a very different plant. In Antigua it has been called the Babricou bean, and in this country has been designated the Pearson bean, and recently the wonder bean. Owing to confusion with the similar species cultivated in Japan, China, and India, it has also been called the sword bean and the knife bean, but those names properly belong to the Asiatic species. The first published description of the plant is by Clusius 2 in 1605, whose figures of the pods and seeds received from Brazil are unmis- « Issued Jan. 18, 1913. 2 Clusius, Carolus. Exoticorum, p. 09, 1005. [Cir. 110] 29 30 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. takable. Clusius, however, describes the seeds as brownish, but all subsequent authors state that the seeds are white, which is the case in all that have been grown in this country. Sloane x gives a good figure and description of the plant in 1707 under the name "horse bean," as he found it in Jamaica. He writes further: They are eaten as other Phaseoli by some and counted good food, though their greatest use is to fatten hogs. Fifty years later Browne wrote of the horse bean as follows : This plant grows in many gardens in Jamaica, where it is cultivated chiefly out of curiosity. It seems to keep a main between the upright and the climbing species of Phaseolus, for the stem seldom rises above 3 or 4 feet, though it emits some slender delicate shoots that run much further. The pods are commonly between 10 and 14 inches in length and generally contain 10 or 11 seeds, but the pulse is very seldom used, being generally thought more or less of a deleterious nature. 2 Macfadyen, writing in the Flora of Jamaica in 1837, records as follows: Sloane considers this species to be indigenous to the island and says the seeds were in his time used by some as food and given to fatten hogs. I do not find, however, on inquiry that any use is made at present of them, except that they are commonly planted by the negroes along the margin of their provision grounds from a supersti- tious notion, probably of African origin, that the overlook fulfills the part of a watch- man and, from some dreaded power ascribed to it, protects the provisions from plunder. Even the better informed adopt the practice, though they themselves may not place confidence in any particular influence this humble plant can exercise, either in pre- venting theft or in punishing it when committed. 3 The same idea prevails at the present day in Panama. BOTANICAL RELATIONSHIPS. The j ack bean was briefly described and named DolieJios ensiformis by Linnaeus. 4 His description is based primarily on the previous account and illustration of Sloane. In later descriptions, however, Linnaeus also included under his species various plants described by other authors. In 1759 he redescribes his Dolichos ensiformis, 5 citing as its basis the plate and description of an Amboyna plant. 6 Linnseus describes the plant as twining, which accords with the descriptions of both Sloane and of Rumphius. In 1763 Linnaeus 7 again describes the plant under the same name, but states that the plant is erect. Besides the plants of Sloane and of Rumphius he includes the Indian plant described and illustrated by Rheede. 8 1 Sloane, Sir Hans, bart. A Voyage to the Islands Madera, Barbados, Nieves, S. Christophers, and Jamaica, with the Natural History, v. 1, p. 177, 1707. 2 Browne, Patrick. Civil and Natural History of Jamaica, p. 291, 1879. 3 Macfadyen, James. Flora of Jamaica, p. 292, 1N37. * Linnaeus, Carolus. Species Plantarum, t. 2, p. 725, 1753. 6 Linnams, Carolus. Systema Naturae, ed. 10. 1. 2, pt. 2, p. 1102, 1759. 6 Rumphius, G. E. Herbarium Amboinense, pars 5, pi. 142, 1747. 7 Linnseus, Carolus. Species Plantarum, ed. 2, t. 2, p. 1022, 1763. s Rheede tot Draakenstem, H. A. van. Hortus Malabaricus, pars 8, pi. 44, 1688. [Cir. 110] THE JACK BEAN AND THE SWOBD BEAN. 31 Finally in 1767 Linnaeus J gives two separate descriptions of Doli- chos ensiformis, in one of which the plant is said to be erect, in the other twining. Whether this was an editorial blunder or whether Linnaeus considered the two distinct and inadvertently used the same specific name twice is open to question. Jacquin evidently thought that the same name covered two species, as the twining one agreed with a plant growing m his greenhouse at Vienna which he had obtained from the West Indies. He redescrlbed and figured it as Dolichos acinaciformis. 2 There can be scarcely a question, however, that these two names include but a single species. It is true Sloane records the plant as twining. Brown says it is suberect, while Mac- fadyen writes "at first suberect, afterward twining." Two varieties were grown at Biloxi, Miss., and Gainesville, Fla., in 1911 and 1912, one early, the other later and larger. The former is suberect and bushy, while the latter has longer, viny branches. Under favorable conditions it would not be surprising for these plants to assume a vining habit, as a similar phenomenon is well known in the case of bush cowpeas and soy beans. The plant of Rheede is a distinct species, Canavali gladiata, and that of Rumphius is probably the same, but with a faulty figure. Later authors have also confused this East Indian plant with the West Indian, so that it is often difficult to determine which species is referred to. The genus Canavali founded by Adanson 3 has received general recognition, and the jack bean is therefore properly known as Canavali ensiformis (L.) DC. Later authors have usually modified the generic name to Canavalia. ECONOMIC VALUE. In the last 20 years the jack bean has several times attracted atten- tion on account of its vigorous growth and large yield of pods and seeds. It was extensively tested at the Mississippi Agricultural Experiment Station during the years 1890 to ,1895. Under field con- ditions yields of 30 to 40 bushels of beans per acre were obtained, even when grown on thin soil. Attempts were made to utilize these beans as feed for both beef and dairy cattle, 4 but the beans were found to be both unpalatable and indigestible. Three of the lots fed with bean meal made a smaller gain than did many of the lots receiving cottonseed meal, and the best of the bean-meal lots, No. 4a, gained only 3.4 pounds more than did the poorest of the lots receiving cottonseed meal. It should be noted that the lots receiving bean meal were also fed cottonseed meal amounting to 1 Einna-us, Carolus. Systems Naturae, ed. 12, t. 2, p. im' 483, 1707. 2 Jacquin, N. J. von. Collectanea, t. 1, p. 114, 1786. 3 Adanson, Michel. Families des Plantes, pt. 2, p. 325, 1763. 4 Lloyd, E. R. , and Moore, J. S. Feeding for beef. Mississippi Agricultural Experiment Station, Bul- letin 39, p. 162-163, 1896. [Cir. 110] 32 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. about one-third of their grain ration, and a large part of their small gain should doubt- less be credited to the cottonseed meal and not to the bean meal. The complete failure to secure profitable results from the use of the bean meal was a surprise and disappointment. Various methods of feeding were tried, both coarse and fine meal being used, and during a portion of the time the meal was cooked until it was thoroughly softened. At first very few of the steers would eat any of the meal, but were finally induced to do so by mixing it with salt and cottonseed meal, so that when the trial feedings began all ate it fairly well, though not with much apparent relish. The meal which was eaten appeared to be very indigestible for all the ani- mals, and the same was found to be the case when it was fed to milch cows, as will be shown in another bulletin, soon to be published. When tried on the table of one of our station stall', the beans were of fair quality, though rather coarse, and no one of the six persons who ate them experienced any ill effect from them. If the different feeds are considered with reference to only single ingredients of the several rations, the average gains per steer were as follows: Pounds. Shredded corn and silage 35. 1 Crab-grass hay 51.5 Pea- vine hay 00. 2 Pounds. Red-clover hay 71.7 Cottonseed meal 02. Bean meal 28. 4 Seeds of the bean were distributed by Mr. P. Pearson, of Starkville, Miss., from which fact it became known as the Pearson bean. At the Texas Agricultural Experiment Station it produced 35 bushels per acre. 1 At the North Carolina Agricultural Experiment Station it produced an estimated yield of 40 bushels per acre. 2 It was also tested at the Louisiana Experiment Station. 3 None of these sta- tions regarded the bean as promising, but, so far as recorded, no attempt was made to utilize either the herbage or the seeds as forage. More recently the plant has been tested in Hawaii, and favorable reports as to its forage value have been published. 4 While grown to some extent in the Southern States, the plant does not appear to thrive as well there as here, and no extensive feeding experiments are reported. The bean meal is said not to be very palatable or digestible for cattle, but this may be due to a too-limited experience in its use. The early feeding experiments with the green fodder in Hawaii gave similar results to those reported above, but as feeders gained in experience the fodder was found to be both palatable and nutritious for dairy cows as well as swine. As with most new feeds, it is important to use in the beginning only a small proportion of the new feed in the accustomed ration and then increase the proportion gradually. The Dowsett and Pond dairies have fed green jack beans and sorghum in equal proportions to dairy cows with excellent results. i Connell, J. H., and Clayton, James. Field experiments at MeKirmey, Wichita Falls, and College sta- tion with wheat, corn, cotton, grasses, and manures. Texas Agricultural Experiment Station, Bulletin 34, p. 584, 1895. 2 McCarthy, Gerald. Some new forage, fiber, and other useful plants. North Carolina Agricultural Experiment Station, Bulletin 133, p. 343, 1896. 3 Dodson, W. R., and Stubbs, W. C. Grasses, clovers, forage, and economic crops. Louisiana Agri- cultural Experiment Station, Bulletin 53, p. 42, 1898. 4 Krauss, F. G. Leguminous crops for Hawaii. Hawaii Agricultural Experiment Station, Bulletin 23, p. 19-21, 1911. [Cir. 110] THE JACK BEAN AND THE SWORD BEAN. 33 The crop requires about a month longer to mature than do cowpeas, but the yield is proportionately greater. Yields of 16 to over 20 tons of green fodder per acre have been reported from various sources. The best yield of seed reported is 1,200 pounds per acre. While a single crop is usually grown from each sowing, the station has occa- sionally grown a good rattoon crop. Such crops, however, are subject to a leaf blight common to the bean family. Otherwise the crop is exceptionally free from diseases and insect pests, a point greatly in its favor over the cowpea. Another possible advan- tage possessed by the jack bean over the rambling legumes is the absence of trailing stems, which might interfere in some forms of intercropping. While the crop is quite drought resisting, as was shown in the excellent yield pro- duced at Kunia during the dry season of 1909, it responds well to irrigation and makes a good growth during the wet season if the weather is not too cold. The jack bean develops a strong root system . The roots are nearly always well supplied with the nodules produced by the nitrogen-fixing bacteria, so that the stubble remaining after the crop is harvested should prove beneficial to the soil. The best method for the culture of the crop, whether it is to be used for green fodder or seed, is to plant in rows and cultivate freely throughout the growing season. .For the largest amount of green matter, plant the seed 3 to 6 inches apart in rows 2 to 3 feet apart. If seed is the object, especially if wanted for planting, the rows should be at least a foot farther apart and the seed planted 6 to 10 inches apart in the row. Forty to sixty pounds of seed will be required to plant an acre. The optimum amount of moisture for seed growing is about two-thirds that required for maximum fodder production. In Porto Rico the jack bean has been found very useful as a green- manure and cover crop in citrus groves. Judging from the behavior of the plant in experimental trials in Florida, it should prove equally valuable there. Its bushy habit makes it especially desirable, as it does not interfere by climbing the trees, while its dense, vigorous growth shades the ground during the heat of summer and provides abundant vegetable matter to add to the soil. The value of the plant as forage is yet problematical. Its successful utilization as green feed in Hawaii encourages the belief that it may be found equally valuable in this country, especially in Texas and Oklahoma, where its great drought resistance gives it particular promise. The large yield of seed per acre justifies further experiments to determine whether any means can be devised to utilize the seeds profitably as feed, which the work referred to of the Mississippi Agri- cultural Experiment Station indicates is a difficult problem. The jack bean has recently been introduced into Java, where on account of the large yield of seed the agricultural authorities were endeavoring to find a market for the product in Europe. There is also the probability that the jack bean may prove to be valuable as ensilage. Its coarse habit and heavy tonnage should adapt it well to this purpose. [Cir. 110] 34 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. Table I. — Analyses of the jack bean. Material. Water. Protein. Fat. Nitrogen- free extract. Crude fiber. Ash. Publication. Seeds Per cent. 11.48 76.81 Per cent. 26.85 2.44 17.76 23. 75 5.21 Per cent. 2.99 .52 3.06 2.65 .48 Per cent. 56.90 48.51 65. OS 50.37 8.44 Per cent. 39. 90 43.89 19.31 8.75 6.36 Per a nt. 3.38 4.64 3.79 3.00 2.70 Miss. Exp. Sta. Hulls Ann. Rep. 8, p. 43, 1895. Do. Whole pods with seeds. Bean meal Do. Miss. Exp. Sta. Green plant Bui. 39, p. 159, 1896. Hawaii Exp. Sta. Bui. 23, p. 31, 1911. THE SWORD BEAN. The sword bean (Canavali gladiata), also known as the knife bean and the saber bean, is closely related to the jack bean and the two have been much confused. The sword bean is found cultivated through much of southern Asia and also in Africa. At various times it has been introduced into America, but is still cultivated almost entirely as a curiosity or as an arbor vine. HISTORY. The sword bean was briefly described and well illustrated by Rheede x under the name "Bara-mareca," the plants being found at Angiecamal and other places on the southwest coast of India as a cultivated vegetable. The color of the beans and seeds is not stated. What is quite certainly the same species is described and figured by Rumphius 2 under the name Lobus machaeroides, the native name in Amboyna being Cacara parrang. The figure of the pods is, however, faulty. Rumphius describes the seeds as either intense red or else fuscous and states it is rarely cultivated in Amboyna but abundantly in Java and Baleya. Linnaeus erroneously supposed the plants of Rheede and of Rumphius to be the same as his Dolichos ensiformis, the jack bean. Jacquin, however, grew the sword bean in the greenhouse of the botanical garden at Vienna and in 1788 described it fully under the name Dolichos gladiatus, 3 later publish- ing a beautiful colored plate. 4 The variety he grew had dark-red seeds and white flowers, which later became suffused with pink. The source of the plant is not stated. Later authors have greatly confused Canavali gladiata and Cana- vali ensiformis, but both the pods and seeds of the two are very i Rheede tot Draakenstein, H. A. van. Hortus Malabaricus, pars 8, p. 85, pi. 44, 1688. 2 Rumphius, G. E. Herbarium Amboinense, pars 5, p. 376, 1747. a Jacquin, N. J. von. Collectanea, t. 2, p. 276, 1788. 4 Jacquin, N. J. von. Icones Plantarum Rariorum, v. 3, pi. 560, 1786-1793. [Cir. 110] THE JACK BEAN AND THE SWORD BEAN. 35 different (PL III). According to Roxburgh four varieties of the sword bean are found in India, one having red seeds and red flowers, a second with red seeds and white flowers, a third with white seeds and white flowers, and a fourth with light-gray seeds and red flowers. Three of these varieties are now introduced into the United States. All of them are supposed by most Indian botanists to be derived from a brown-seeded wild plant, Canavali virosa, the seeds of which are reputed poisonous. Macfadyen reports the variety with red flowers and red seeds as cultivated in Jamaica in 1837. This variety does not fully mature as far north as Washington, D. C, but does produce an abundance of green pods in late September and early October. It seems well worthy of attention as a vegetable through- out the Southern States. ECONOMIC VALUE. The sword bean is commonly cultivated as a vegetable in Japan, India, Burma, Ceylon, Java, Mauritius, and apparently in Africa. In India it is eaten both by the natives and by Europeans, the variety with white seeds being .most esteemed. The young pods are prepared after the manner of snap beans and are well flavored and wholesome. Firminger considers it "about the nicest of all the native vegetables" in India. The very young pods have but little flavor, but when half grown their taste suggests mushrooms. They are best when about half grown, as the full-sized green pods are rather fibrous. The mature seeds do not seem to be much used as food, though they lack the strong odor of those of the jack bean. An extended account of the sword bean as grown in Mauritius has been published by Boname, 1 but under the erroneous name Canavali ensiformis. This author highly recommends the green pods as a vegetable and gives analyses showing the composition not only of the entire plant but of the beans and pods, both green and ripe. In China and Japan occur two varieties of the sword bean which differ from those of India in that the pods are thicker and the seeds much plumper and distinctly keeled on the back (PL III). This form was described by Thunberg 2 as Dolichos incurvus and by Petit- Thouars as Canavali incurva. The Japanese name is Nata mame and the Chinese Tau tou. One variety has the seeds cream colored, another pink. In Japan "the young pods of the former are pre- served in salt, and the latter is eaten fresh and boiled." 3 Rev. E. R. Miller, of Morioka, Japan, in sending a sample of seed (S. P. I. No. 6132) writes "This as a string bean eaten when very young is one of 1 Boname, P. The sword bean. L'Agriculture Pratique des Pays Chauds, arm. 10, sem. 2, p. 371, 1910. 2 Thunberg, K. P. Flora Japonica, p. 280, 1784. 3 Agricultural Society of Japan. Useful Plants of Japan, [v. 1], p. 9, 1S95. [Cir. 110] 36 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. the finest I ever tasted. * * * The Japanese use them generally for pickling when young and they are very fine for this purpose." In China the pink-seeded form (S. P. I. No. 23216) is said by Mr. Frank N. Meyer to be "a very rare bean used mainly as a stomach- strengthening food, and for this reason to be had only in medicine shops." Brill, under S. P. I. No. 6570, also records that these "beans are very good but expensive." As the plant yields heavily, it is difficult to understand why the beans should be expensive. VALUE FOR FORAGE AND AS A COVER CROP. All the varieties of the sword bean tested are rambling vines, none of them being bushy like the jack bean. As forage they are not as desirable, as the foliage is just as bitter and the habit inferior. As a cover crop the Indian variety with red seeds and red flowers has proved very satisfactory in Porto Rico. Cattle are said to graze on the plant there to a limited extent. The sword bean is not infrequently seen in the South, grown as an arbor plant, but little seems to be known of the value of the green pods as a vegetable. Indeed, the impression prevails that the seeds are deleterious, an idea doubtless obtained by association with the very similar jack bean, as the sword bean is everywhere utilized as a vegetable in the Orient. The plant will develop full-grown green pods as far north as Washington, D. C, but ordinarily the season is not long enough for the seeds to ripen. [Cir. 110] FIBER FROM DIFFERENT PICKINGS OF EGYPTIAN COTTON. 1 By Thomas H. Kearney, Physiologist in Charge of Alkali and Drought Resistant Plant Investigations. INTRODUCTION. Two pickings were made from the individual selections of the Yuma variety of cotton at Sacaton, Ariz., in 1911, the first early in October, the second about a month later. The first picking consisted largely of the first bolls which open on the plants, these being borne principally on the lower fruiting branches of the main stem. The development of these lower bolls is frequently checked by the heavy shading to which the lower part of the plant is subject in this variety. Practically all of the bolls included in the first picking had matured and opened during the hot weather in September. The second, or November, picking took in the cotton from bolls which were more favorably situated on the plants (nearer the tops) and which had matured during the cooler weather of October. At Bard, Cat., in 1911, the first cotton in the progeny rows of the Yuma variety ripened so early that these rows were picked in bulk about the middle of September, before individual selections had been made, thus eliminating the cotton which was included in the first individual picking at Sacaton — that from the early-opening bolls near the bases of the plants. For this reason, it would be expected that the cotton of the two pickings made from the individual selections would show less difference at Bard 2 than at Sacaton. A comparison of the fiber from the two pickings of each individual plant at Sacaton and at Bard, respectively, proved that this was the case. No differences in length, strength, and abundance (lint index) of fiber, or in weight of seeds, could be detected in samples from the two pick- ings at Bard. The conclusions herein reached are therefore based solely upon the material collected at Sacaton. i Issued Jan. 18, 1913. 2 At Bard the first picking of individual selections was made about October 15, the second at the end of November. [Cir. 1101 37 38 CIRCULAR NO. 110, BUREAU OF PLANT INDUSTRY. COMPARISON OF THE DIFFERENT PICKINGS. LENGTH OF STAPLE. In 33 out of the total number of 60 individual selections made at Sacaton, careful measurement and comparison of the length of staple in the first and in the second picking, respectively, were made. 1 The fiber from the second picking of these 33 plants averaged one-sixteenth of an inch longer than that from the first picking. The uniformity of length was also greater hi the second picking. In 42 selections in which the variation in length was recorded, it averaged three- sixteenths of an inch hi the first picking and two-sixteenths of an inch in the second picking. In 30 of these plants the variation was greater in the first picking, hi 6 plants it was greater in the second picking, and in the remaining plants it was the same in both pickings. STRENGTH OF FIBER, In 44 individual selections hi which the first and second pickings were compared in respect to strength, 27 showed a greater strength of fiber in the second picking than hi the first, 13 showed no marked difference between the two pickings, and only 4 showed stronger fiber in the first picking. LINT INDEX. The fiber was decidedly more abundant in the second picking than in the first, the average lint index (weight in grams of fiber per 100 seeds) in the 60 individual selections having been 4.90 for the first picking and 5.17 for the second picking. FINENESS OF FIBER. It has been observed every year during which breeding work with Egyptian cotton has been carried on in the Southwest that the fiber from the bolls which open first is often coarser and harsher to the touch than the fiber produced in bolls higher on the plant, which ripen later under the influence of more equable temperatures. WEIGHT OF SEEDS. The average weight of 100 seeds in the 44 individual selections in which the two pickings were compared in this respect was 12.9 grams for the first picking and 13.4 grams for the second picking, indicating that the seeds produced in the bolls which ripen earliest are decidedly lighter than those hi later ripening bolls borne higher on the plant. 1 The measurement of length in each of the two pickings was made as follows: From the sample of seed cotton representing each picking from a given individual plant, 10 seeds were drawn at random from different portions of the mass. A cluster of fibers from near the middle of each seed was pulled out and the average length of these fibers was estimated. The mean of the average lengths of the fiber from the 10 different seeds was taken as representing the length of staple for the picking in question. [Cir. 110] FIBER FROM DIFFERENT PICKINGS OF EGYPTIAN COTTON. 39 FUZZINESS OF SEEDS. There was some indication that the seeds from the first picking had a tendency to develop a rather larger quantity of fuzz on the seed coat than was the case in the second picking, but it is by no means certain that any noteworthy or constant difference in this respect exists. CONCLUSIONS. It is clear from the foregoing that the earliest ripening bolls (chiefly those near the base of the plant), which open while extremely high temperatures still prevail, are likely to contain less abundant, shorter, weaker, coarser, and less uniform fiber than bolls which ripen later. The cotton from the earliest picking is also likely to contain more dust and "trash," since the bolls which produce it are closer to the ground and since the leaves and bracts around them have often dried up and become very brittle before the picking is made. For these reasons it would seem advisable, in order to maintain the high- est possible standard for Egyptian cotton grown in the Southwest, to make the first picking as early as the number of open bolls will war- rant the expense of the operation and to sell the fiber from this pick- ing as a separate grade, not allowing it to become mixed with the bulk of the crop, which may be expected to show decided superiority in grade and quality. It is also probable that the latest cotton to ripen, especially that contained in bolls which open after a severe frost, should also be graded separately. The investigation here described does not, however, throw any light upon this latter point. 1 Since the bolls which ripen first have often cracked open prema- turely under the influence of the high temperatures and extremely dry winds which usually characterize the month of September in the Southwest, the seeds contained in them are frequently not thoroughly mature. This is indicated by their smaller average weight and in fact is evident to the eye from their frequently lighter color. Although no experiments have yet been made to test the point, it is not unlikely that these seeds will be found to give a lower percentage of germination and to produce less vigorous plants than seeds from the second picking. In such cases it would be advisable to use the latter as much as possible for planting. 1 It is interesting to note in this connection that spinners and buyers to whom samples of the 1910 crop of Arizona-grown Egyptian cotton were submitted found the fiber to be weaker in the sample which repre- sented the fourth picking than in samples which represented earlier pickings from the same fields. LOir. 110] o U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 111. B. T. GALLOWAY, Chief of Bureau. MISCELLANEOUS PAPERS. Preliminary Report on Sugar Production from Maize Durango Cotton in the Imperial Valley Improved Apparatus for Detecting Sulphured Grain Supernumerary Carpels in Cotton Bolls Keeping Soft Cuttings Alive for Long Periods . C. F. CLARK 0. F. COOK G. H. BASTON R. M. MEADE G. W. OLIVER Issued February 1, 1913. WASHINGTON: GOVERNMENT PRINTING OFFICE. 1913. [Cir. Ill] 2 BUREAU OF PLANT INDUSTRI, Chief oj Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A. Taylor. Editor, J. E. Rockwell. Chief Clerk, James E. Jones. PRELIMINARY REPORT ON SUGAR PRODUCTION FROM MAIZE. 1 By C. F. Clark, Assistant Agronomist, Office of Cotton and Truck Disease and Sugar- Plant Investigations. INTRODUCTION. The experiments described in the following pages were conducted under the direction of Mr. W. A. Orton, of the Bureau of Plant Industry, in cooperation with the Sugar Laboratory of the Bureau of Chemistry. The work was carried on during the summer of 1912 at Garden City, Kans., and Washington, D. C. At Garden City the field work was in charge of the writer and the analytical work under the direction of Mr. W. B. Clark, assisted by Messrs. R. J. Hamon and C. A. Hauser. At Washington the experimental plats, which were located on the Arlington Farm, were under the supervision of Mr. J. I. Milstead, of the Office of Horticultural Investigations, while the analytical work was performed under the direction of JVIr. A. Hugh Bryan, of the Bureau of Chemistry. These preliminary studies have been carried out with only two varieties of corn. Other varieties might give more or less favorable results. It is well known that the maize plant responds to selection for specific purposes, and it is possible that results achieved in the selection of the beet to increase its sugar content might be paralleled with the proper selection of maize. EXPERIMENTS AT GARDEN CITY. The variety used was Stowell's Evergreen sweet corn. It was planted on May 15 and the ears were removed on August 9, when in the milk stage. For comparison alternate rows were left with the ears on. These stalks were analyzed at the same time as those from which the ears had been removed. Analyses were made at frequent intervals extending from August 28 to September 25. The samples, which consisted of 10 stalks each, with the exception of the one analyzed September 7 which contained 30 stalks, were taken on the day in which the analyses were made. As soon as cut the stalks were taken to the laboratory and the juice extracted by running twice through a 3-roller mill. The solids were determined by the Brix spindle and the apparent sucrose by direct polarization, from which data the apparent purity was calculated. The results are given in Table I. i Issued Feb. 1, 1913. [Cir. Ill] 3 4 CIECULAR NO. Ill, BUREAU OF PLANT INDUSTRY. Table I. — Composition of cornstalks grown and analyzed at Garden City, Kans. 1 Date of analysis. Field treatment. Solids. Differ- ence. Sucrose, direct polariza- tion. Differ- ence. Appar- ent purity. Differ- ence. Aug. 28 Sept. 3 Sept. 5 Sept. 7 Sept. 9 Sept. 18 Sept. 25 /Ears removed \Ears not removed. /Ears removed (Ears not removed . fEars removed \Ears not removed. Ears removed /Ears removed (Ears not removed. /Ears removed \Ears not removed. (Ears removed \Ears not removed . Per cent. 15. 45 11.70 15.80 8.90 16.80 11.55 16.60 16.25 10.95 16.17 13.22 17.54 12.87 Per cent. | 3.75 }■ 6.90 !■ 5.25 Per cent. 9.76 5.77 5.30 2.95 4.67 Average increase following removal of ears 4.80 3.65 11.33 5.96 10.85 10.65 4.60 10.03 7.41 s 10. 50 2 6.50 Per cent. \ 3.99 \ 6.33 \ 5.37 Per cent. 63.20 49. 30 / 63.20 \ \ 49.30 J Per cent. 13.90 63.20 41.00 / 67.40 \ \ 51.60 / 22. 20 15.80 | 6.05 | 2.62 } 4. 00 65.40 65.60 42.00 62.00 56.15 2 59. 86 2 50. 50 4.73 23. 60 5.85 9.36 15.12 1 Analyses made at the Garden City Field Laboratory of the Office of Sugar-Plant Investigations. 2 These results are from Hauser's analyses; they seem to have been calculated from Clerget polarizations, in which case the values for sucrose and purity would be true instead of apparent, which would make both sets of figures higher, relatively, than those of the preceding analyses. The data presented in Table I show that throughout the entire period covered by the analytical work there was a marked increase in the solids, in sucrose, and in purity as a result of removing the ears, the average increase in each case being 4.80, 4.73, and 15.12 per cent, respectively. In no instance was there a decrease. It will be seen that in the stalks from which the ears had been removed there was an increase in the percentage of sugar from the first analysis until September 5, when the maximum was reached. After this there was a small, though fairly uniform, decrease until September 25, when there was a slight increase. EXPERIMENTS AT WASHINGTON. FIELD DATA. For the experiments at Washington, D. C, a dent corn was used, which was a selection of the Boone County White. It was planted about May 30 and the ears were removed on August 22. The follow- ing data were obtained regarding the weight of ears and the cost of removing the same: Weight of ears from one-third acre 2, 754 pounds. Time required to remove ears from one-third acre 5 hours. Time required to pick up ears and carry them from field 3 hours. Total cost of removal of ears from one-third acre (8 hours, at 15 cents) $1. 20. ANALYTICAL METHODS AND RESULTS. Analyses of the stalks from which the ears had been removed, as well as from those on which the ears remained, were made on Tuesday and Thursday of each week from August 27 to October 29. The REPORT ON SUOAR PRODUCTION FROM MAIZE. samples consisted of 24 stalks each and were taken the morning of the day the analyses were made. As soon as cut they were sent to the Bureau of Chemistry, where the juice was immediately extracted by running once through a 3-roller mill. The following analytical determinations were made of this juice: (1) Solids by Brix spindle, (2) direct and invert polarizations, (3) sucrose by Clerget, (4) invert sugar before reduction, and (5) percentage of moisture in the sample of cane as originally received. The purity of the juice — that is, the percentage of sucrose divided by the solids — was calculated from the data thus obtained. The results are presented in Table II, the sum- mary of which shows the changes in composition of the juice follow- ing the removal of the ears. Table II. — Composition of cornstalks grown and analyzed at Washington, D. C l EARS REMOVED. Serial No. Juice. Cane. Date. Solids. Su- crose. Invert sugar. Non- sugar solids. Purity. Water. Su- crose. Invert sugar. 1912. Aug. 27 Percent. 9728 9736 9740 9758 9769 9773 9791 ■ISOI) 9817 9823 9837 9840 9854 9871 9939 9956 10005 10025 10072 Percent. 12.78 12.46 14.11 14.50 15.32 16.81 16.25 15. 64 14.68 9.50 la 07 15. 30 15.50 15.52 11.22 10.45 15.83 13. 87 15.22 Percent. 7.58 6.94 8.58 9.11 9.54 11.27 10.70 10.18 9.42 4.86 9.42 9.72 10. 02 10.10 6.40 5.56 9.46 7.61 9.01 Percent. 2.88 2.90 3.00 2.24 2.33 1.98 1.74 1.69 1.51 1.67 1.85 1.84 1.70 1.76 1.62 1.92 2.26 2.55 2.38 Percent. 2.32 2.62 2.53 3.15 3.55 3.56 3.81 3.77 3.75 1.97 3.80 3.74 3.78 3.66 3.20 2.97 3.11 3.71 3.83 Percent. 59.3 55. 7 60.8 62.8 62.3 67.0 65.9 65.1 64.2 51.1 62.5 63.5 64.6 65.1 57.0 52.2 59.8 54.9 59.2 Percent. Percent. Percent. 29 Sept. 3.. 73.94 74.56 71.86 73.91 70.58 74.64 66.40 75.97 70.79 70.54 69.88 72. 74 78. 70 71.03 69.35 67.36 67.01 7.38 7.94 8.09 10.01 9.02 9.01 7.33 4.08 7.85 8.09 8.28 8.70 5.67 4.40 7.80 5.95 7.12 2.58 5 1.95 10 1.98 12 1.76 17 1.46 19 1.49 24... 1.17 26... 1.40 Oct. 1 3 1.54 1.53 8 1.40 10 1.51 15 1 43 17 1.52 22 1.86 24... 1.99 29... 1.88 Average 14.21 8.71 2.09 3.31 60.68 71.73 7.45 1.67 EARS NOT REMOVED. Aug. 27. . 29. Sept. 3. 5. 10. 12. 17. 19. 24. 26. Oct, 1 . 8. 10. 15. 17. 22. 24. 29. Average . 9729 9737 9741 9759 9770 9774 9792 9801 9818 9824 9838 9841 9855 9872 9940 9957 lOOOo 10026 10073 10. 08 8.41 7.50 8.70 6.45 0.30 8.38 6. 99 7.48 5.97 6.09 6.87 9. 03 8.95 8.07 10. ON 10.21 9.05 9.35 8.13 4.08 3.13 2.64 3.53 1.81 1.88 3.39 2.53 2. 53 1.63 1.55 2.34 4.24 4.24 3.18 5.18 6. 86 4.45 3.73 3.31 3.49 2. 7( '. 2.51 2.92 1.90 1.80 2.32 1.80 1.83 1. 01 1.42 1.54 1.93 1.91 1.92 2.35 1.96 1.96 2.57 2.13 2.51 2.52 3.12 2.99 2.80 2. SO 2.97 2.55 1.39 3.24 3. 05 2. 69 24.7 37. 2 35.2 40.7 29.0 30.0 40.3 36.2 33. 9 27.3 25.4 34.0 46. 9 47.4 39.4 51.3 67.1 41 i. 1 38.55 80.35 79.30 82.94 80.42 75. 8G 82.56 72.46 80.60 81.42 81.94 70.90 76. 52 77.10 77.66 69. 75 76. 41 73.52 77.98 2.29 3. 06 1.60 1.61 2.80 2.24 1.98 1.39 1.34 2.06 3.58 3.56 2. 66 4.47 5. 32 3. 76 3. 03 2.75 2.18 2.53 1.73 I . .". 1 1.92 1.59 1.43 1.38 1.23 1.35 1.63 1.60 1.61 2.03 1.52 1.66 2.08 1.71 1 Analyses made by Sugar Laboratory, Bureau of Chemistry. CIRCULAR NO. Ill, BUREAU OP PLANT INDUSTRY. Table II. — Composition of cornstalks grown and analyzed at Washington, D. C. — Con. SUMMARY SHOWING CHANGES IN COMPOSITION OF JUICE FOLLOWING REMOVAL OF EARS. Date of analysis. Aug. 27 29 Field treatment. /Ears removed \Ears not removed (Ears removed \Ears not removed c . o /Ears removed aepi. 6 -i Ears not removed . |/Ears removed \Ears not removed 10 12 17 19 24 /Ears removed \Ears not removed (Ears removed. \Ears not removed (Ears removed (Ears not removed /Ears removed \Ears not removed /Ears removed. . \Ears not removed „„ /Ears removed. . . i\Ears not removed Oct. 1 3 8 10 15 17 22 24 29 /Ears removed \Ears not removed /Ears removed.. . \Ears not removed /Ears removed. . . \Ears not removed /Ears removed.. . \Ears not removed /Ears removed . . . \Ears not removed Ears removed. . . t Ears not removed /Ears removed... \Ears not removed /Ears removed. . . \Ears not removed /Ears removed . . . (Ears not removed Average increase or de crease (— ) following removal of ears Solids. Per cent. 12.78 10.08 12.46 8.41 14.11 7.50 14.50 8.70 15.32 6.45 16.81 6.30 16/25 8.38 15.64 6.99 14.68 7.48 9.50 5.97 15.07 6.09 15.30 6.87 15.50 9.03 15.52 8.95 11.22 8.07 10.45 10.08 15.83 10.21 13.87 9.65 15.22 9.35 }5.80{ Differ- ence. Per cent. \ 2.70 4.05 | 6.61 5.80 }• 8.87 J-10. 51 | 7.87 } 8.65 | 7.20 Su- I Differ-jlnvert crose. ence. sugar. Per cent. 7.58 08 {1 6.94 { 3.13 / 8.58 \ 2.64 9.11 3.53 / 9.54 \ 1.81 ft /10. 18 \ 2.53 i 9.42 \ 2.53 }, }, }«■ | 6.47 ), ) I ) }<■ }, 4.86 1.63 / 9.42 \ 1.55 57 3.15 .37 5.62 22 s7 6.08 '-'7 10.70 39 9.72 2.34 10.02 4.24 110.10 \ 4.24 / 6.40 \ 3.18 {I {I: { 5.56 18 9.46 86 61 45 9.01 3.73 Per cent. | 3.50 3.81 5.94 5.58 | 7.73 } 9.39 } 7.31 7.65 | 6.89 | 3.23 | 7.87 7.38 5.78 \ 5.86 | 3.22 } .38 2.60 3.16 } 5.28 / 1.74 \ 2.32 5.40 Per cent. 2.88 3.49 2.90 2.76 3.00 2.51 2.24 2.92 2.33 1.96 1.98 1.80 Per cent. }•» }■ h } {?•" 1.69 1.80 1.51 1.83 1.67 1.61 1.85 1.42 1.84 1.54 1.70 1.93 1.76 1.91 1.62 1.92 1.92 2.35 2.26 1.96 96 2.38 2.57 Differ- ence. 68 .37 18 }■ \— .58 h" }-.32 } .06 .43 .30 ( > |— .23 }-'= | — .30 } .30 }• }- .7.1 L9 -.04 Non- su?ar solids. Per cent. 2.32 2.51 2.62 2.52 2.53 2.35 3.15 2.25 3.55 2.68 3.56 2.62 3.81 2.67 3.77 2.66 3.75 3.12 1.97 2.73 3.80 3.12 3.74 2.99 3.78 2.80 3.66 2.80 3.20 2.97 2.97 2.55 3.11 1.39 3.71 3.24 3.83 3.05 Differ- ence. Per cent. }... }.» | .90 j .» | .94 } 1.14 },.u I .03 }-,« | .68 (.,5 92 { { / 60. \ 35. / 62. \ 40. { { / 65. \ 40. !■ >'■ >■ }■ >'• }- }- .02 Pur- ity. Per cent. 59.3 24.7 55.7 37.2 62.8 7 62.3 29.6 67.0 30.0 65.9 3 65.1 36.2 64.2 9 51.1 3 02.5 4 / 04. \ 33. I 51. \ 27. / 02. \25. / 63.5 \ 34.0 / 64.6 \ 46.9 / 65.1 \ 47.4 / 57.0 \ 39.4 / 52.2 ( 51.3 59.8 1 / 59. \ 67. / 54. \ 46. / 59. \ 39. 54.9 1 59.2 Differ- ence. Per cent. 34.6 18.5 25.6 22.1 • 32.7 ■ 37.0 25.6 28.9 30.3 23.8 37.1 29.5 | 17.7 } 17.7 } 17.6 } ■• | -7.3 | 8.8 } 19.4 22. 13 The summary of Table II shows that very marked changes were produced in the composition of the juice as a result of the removal of the ears. Each analysis showed an increase in solids, in sucrose, in nonsugar solids (with two exceptions), and in purity (with one exception). Of the 19 determinations for invert sugar 9 showed an increase and 10 a decrease, the average of which ( — 0.04 per cent) is so small as to be considered negligible. [Cir. Ill] REPORT ON SUGAR PRODUCTION FROM MAIZE. 7 If we compare the averages of all determinations which are brought together in the table, it will be seen that the greatest effect resulting from the removal of the ears is on the sucrose content. While there was a substantial gain in nonsugar solids, the increase was not nearly so great as in the case of the sucrose. The percentage of invert sugar, as has already been pointed out, was scarcely affected. The sugar content where the ears had been removed (Table II) increased at a very uniform rate from the date of the first analysis until September 12, when the maximum was reached. After this there was a decrease, which, however, was subject to considerable fluctuation. It will be noted that the last sucrose determination (9.01 per cent) is somewhat higher than the average for the entire season (8.71 percent). The percentage of invert sugar was highest during the early part of the season, after which there was a decrease, followed by an in- crease toward the end of the season. That the accumulation of sugar is affected very quickly by the removal of the ears is indicated by the fact that the analysis of August 27, which was only five days after the ears were removed, showed an increase in sugar content of 3.5 per cent. PRESSING, CLARIFICATION, AND EVAPORATION. The experiments included under this heading were conducted by Mr. Bryan, who reports as follows: EFFECT OF SEPARATE PRESSINGS. In order to study the effect of extra pressing, a sample of cornstalks was run through the mill and the juice collected. The stalks were again run through the mill and this juice collected. The pressed cane coming from this second pressing was mois- tened with water and then run through the mill. This juice was collected. The juice coming from the three pressings was analyzed separately; then all mixed together and the mixed juice analyzed. The results of this work are shown in Table III. Table III. — Composition of juice from separate pressings. Juices analyzed. Juice from first pressing. . . Juice from second pressing Juice from maceration Mixed juice Solids. Percent. 14.76 15.36 9.81 13. 41 Sucrose. Per cent. 10.02 10.17 6.56 8.97 Invert sugar. Per cent. 1.82 1.63 .93 1.56 Ash. Per cent. 0.66 .68 .45 Nonsugar solids. Per cent. 2.26 2.88 1.87 2.30 Purity. Percent. 67.9 66.2 66.9 66.8 It will be noted that the juice from the second pressing contains slightly more solids and also sucrose than the juice of the first pressing. At the same time it is much higher in nonsugar solids, which gives it a lower purity. This fact is always noted in cane-sugar manufacture, viz, that the purer juice comes from the first pressing and as pressing increases the juice becomes more impure. [Cir. ill] CIRCULAR NO. Ill, BUREAU OF PLANT INDUSTRY. CLARIFICATION AND EVAPORATION. To the mixed juice, milk of lime was added to a slight alkalinity, as shown by litmus paper. The lime used contained about 40 per cent magnesium. The whole was then heated nearly to boiling or to a point where the scum easily separated from the clear juice. The heat was then turned off and the juice allowed to settle. The clear juice was then filtered off, brought to a boil, and skimmed. In order to test the effect of concentration in acid solution, water saturated with sulphur dioxid was then added to the boiling juice until it was decidedly acid to litmus paper. The whole was then evaporated to a sirupy consistency. The analy- sis of the juice after sulphuring but before evaporation showed the following com- position: Table IV. — Analysis of juice after sulphuring. Juice. Solids. Sucrose. Invert sugar. Ash. Nonsugar solids. Purity. After sulphuring but before evaporation Percent. 14.96 Per cent. 10.10 Per cent. 1.79 Per cent. 0.71 Per cent. 2.36 Percent. 67.5 By the use of lime and sulphur dioxid it is seen that there had been a rise in purity, which indicates a clarification by removal of some of the organic material. When this concentrated material had reached a proper density, as told by appearances, it was allowed to cool slowly. Crystals of sucrose did not appear immediately, but after a week well-defined crystals of sucrose were noted. These continued to grow. COMPARISON OF CORN WITH SORGHUM AND SUGAR CANE. In order to show the relative value of corn as a source of sugar in comparison with other sugar-producing plants belonging to the grass family, Table V has been prepared. Table V. — Comparison of corn with sorghum and sugar cane as a source of sugar. Plant source. Solids. Sucrose. Invert sugar. Nonsugar solids. Purity. Corn: Grown at Garden City, Kans.— Ears removed Per cent. 16.37 11.53 14.21 8.13 14.11 15.30 17.86 Per cent. 10.44 5.65 8.71 3.31 9.11 12.15 15.59 Per cent. Per cent. Per cent. 63 81 Ears not removed 48.43 Grown at Washington, D. C— Ears removed 2.09 2.13 .82 1.35 .43 3.31 2.69 " 4.18 1.78 1.84 60.68 Ears not removed 38. 55 Sorghum ' 64.48 Sugar cane: Louisiana 2 79.19 Hawaii 3 87.28 i Average of analyses of 20-stalk samples of four varieties (Early Amber, Coleman, Orange, and McLean) grown at Garden City, Kans., in 1912 in the same field as the corn. 2 Average of analyses of D. 74, D. 95 and home canes for nine years, 1895 to 1903. See Stubbs, W. C, and Blouin, R. E., Comparative results of seedling sugar canes, D. 74 and D. 95, with our home sugar canes (Louisiana Striped and Louisiana Purple), Louisiana Agricultural Experiment Station Bulletin, s. 2, no. 78, p. 7-11, [1904]. 3 Average of analyses of 17 varieties of plant cane harvested in 1904 and ratoon crop from the same plant- ing harvested in 1906. See Eckart, C. F., Comparative tests with varieties of sugar cane, Hawaiian Sugar Planters' Association, Division of Agriculture and Chemistry, Bulletin 17, p. 6. The corn grown at Garden City from which the ears had been removed was higher in solids than either sorghum or Louisiana [Cir. in j REPORT ON SUGAR PRODUCTION FROM MAIZE. 9 sugar cane, but lower than Hawaiian cane. The corn grown at Washington was slightly higher than the sorghum in solids, but lower than either Louisiana or Hawaiian sugar cane. At Garden City the sucrose content was higher in the corn than in sorghum, while at Washington the opposite condition was found. In both instances it was lower than in sugar cane. The percentage of invert sugar was higher in corn than in either of the other plants. The nonsugar solids were lower in corn than in sorghum, but higher than in sugar cane. The purity was lower in corn from both locali- ties than in sorghum or sugar cane. SUMMARY. The results of the present season's work show that by the removal of the immature ears from cornstalks the sucrose content of the juice is greatly increased. There is also a small increase in non- sugar solids and a very pronounced increase in purity. The per- centage of invert sugar is not materially affected. The purity coefficients of the juices are relatively low, the highest being 67. 4 per cent. As a sugar-producing plant corn compares very favorably with sorghum in the chemical composition of its juice, but it is much lower than sugar cane in sucrose and purity. 74943°— Cir. 111—13 2 DURANGO COTTON IN THE IMPERIAL VALLEY. 1 By 0. F. Cook. Bionomist in Charge of Crop Acclimatization and Adaptation Investigations. INTRODUCTION. Duraiigo cotton is a new type of long-staple Upland cotton recently introduced and acclimatized by the United States Department of Agriculture. The behavior of the Durango cotton in numerous experimental and field plantings leaves no doubt that the variety is well suited to the irrigated lands of the Imperial Valley of southern California. Other long-staple varieties have given excellent results when the conditions happened to be favorable, but the Durango has shown a wider range of adaptation and has produced good crops in places where other long-staple cottons were comparative failures. The success of the new variety has aroused popular interest and is stimulating the development of a long-staple cotton industry in southern California. There is every reason to expect that long-staple cotton will become one of the chief products of irrigation farming in the South- west. In addition to very favorable natural conditions and the absence of boll weevils, the cotton growers of the irrigated districts are more ready to avail themselves of the many advantages that are to be secured through the organization of local cotton-growing asso- ciations for the production of a single superior type of cotton in the community. 2 DEVELOPMENT OF DURANGO COTTON. Durango cotton is one of several varieties developed by the De- partment of Agriculture with a view to mitigating the injuries of the boll weevil in Texas and adjacent States. The probability that the weevil would seriously interfere with the cultivation of the late- maturing types of long-staple Upland cotton formerly grown in the Red River Valley and in the Yazoo Delta region of Mississippi and Louisiana was foreseen several years before the long-staple districts 1 Issued Fob. 1, 1913. 2 These advantages have been considered in detail in an article published in the Yearbook of the Depart- ment of Agriculture for 1911 under the title "Cotton improvement on a community basis." Separate reprints of this article can be obtained by application to the Superintendent of Documents, Government Print ing Oilier, Washington. D. C, upon the payment of a fee of 5 cents each. [Cir. HI] H 12 CIRCULAR NO. Ill, BUREAU OF PLANT INDUSTRY. were actually invaded by the weevils. It was feared that the weevil invasion might result in the complete destruction of the long-staple industry unless earlier and more prolific varieties could be developed. The Columbia, Foster, and Durango varieties may serve to illus- trate three different methods that were employed by the Department of Agriculture in the effort to develop earlier long-staple sorts. The Columbia cotton was obtained by straight selection from a short- staple variety, the Foster by crossing long and short staple varieties, and the Durango by acclimatization and selection of an imported stock. Several other promising types of Upland cotton have been secured by the Department of Agriculture from weevil-infested regions in Mexico and Central America and acclimatized in the United States on account of special characters or habits that render them less sus- ceptible to weevil injury. The Durango is the first of these to attain sufficient uniformity to justify general distribution. The fact that the Durango cotton has weevil-resisting characters is not important, of course, in the Imperial Valley, where there are no boll weevils; but the variety has other good qualities, quite apart from weevil resist- ance. That valuable new varieties should be found in Mexico and other parts of tropical America should not appear surprising in view of the fact that most of the types of cotton now cultivated in the United States appear to have been introduced from tropical America. The so-called Texas big-boll varieties are supposed to have been derived from a Mexican stock introduced only a few decades ago. The intro- duction of the Sea Island cotton from the West Indies is also a matter of history. The acclimatization of Durango cotton was begun seven years ago, in 1905, when the original stock of seed from the State of Durango, Mexico, was planted for the first time in Texas. The superior type represented by the present select strain was first recognized and iso- lated at Del Rio, Tex., in October, 1907. The introduction of the variety into the Imperial Valley occurred in 1911, when a field of about 3 acres was grown on the farm of Mr. W. E. Wilsie, near El Centre About 200 acres were planted in 1912 in the vicinity of El Centro and Holtville. Recent advices state that a carload of the Durango cotton has been sold at El Centro at 17^ cents per pound, which is equivalent to about 19 cents in eastern markets. CULTURAL CHARACTERS OF DURANGO COTTON. Durango cotton is valuable because it represents an unusual com- bination of desirable cultural characters with length and high quality of staple. From the agricultural point of view all of the well-known [Car. -Ill] DURANGO COTTON IN THE IMPERIAL VALLEY. 13 » long-staple Upland varieties have appeared at a serious disadvantage in comparison with the short-staple varieties in heing later and less productive and more difficult to pick because of the smaller size of the bolls. But Durango cotton is as early, as prolific, and as easy to pick as most of the short-staple varieties that are now being grown in the cotton belt. In the Imperial Valley the Durango cotton has outyielded the short -staple varieties, in addition to producing a much more valuable fiber. The only varieties of short-staple cotton that can be said to com- pete with the Durango cotton in the Southwest belong to the Texas big-boll type, such as the Rowden, Triumph, and Lone Star. These varieties have larger bolls than the Durango cotton, but are distinctly inferior in habits of growth. When the conditions favor a luxuriant development of the plants, as they usually do in the Imperial Valley, the Texas big-boll varieties appear at a special disadvantage on account of the weaker stalks and heavier foliage. The Triumph cotton, which has been the principal short-staple variety in the Imperial Valley, often becomes entirely prostrate before the picking season arrives, or hides the bolls under a dense mass of foliage. It is often necessary to hold up the plants with one hand in order to pick the cotton with the other. The heavy shade also tends to keep the bolls moist, and many of them are rotted or mildewed. The Durango variety has stronger stalks. The plants maintain a more erect position, with the bolls much more readily accessible to the picker. (Fig. 1.) The lighter and more open foliage renders the Durango cotton less susceptible to rotting of the bolls or to discoloration of the fiber by con- tact with the soil or by molding under heavy shade. The contrast in foliage characters and habits of growth between the Durango and Tri- umph is so striking that some of the Imperial Valley growers were able to pick the two varieties separately in fields that had been planted with mixed seed. Even the most unskillful picker could see the difference, between the small, low, or prostrate Triumph, densely cov- ered with large, leaves, and the large, upright Durango plants, with their more open foliage and larger crops of bolls. DURANGO COTTON COMPARED WITH THE COLUMBIA. Several varieties of Upland long-staple cotton have, been tested in the Imperial Valley in experimental plantings, so as to permit close comparisons to be made between adjacent rows of the different sorts. In this way it is possible to secure a much clearer idea of differences of behavior than can be gamed from plantings hi separate fields. Under the Imperial Valley conditions Durango cotton has shown marked superiority over the Columbia both in yield and in quality of fiber. In 1911, 4 bales of Durango cotton were grown on less than [Cir. Ill] 14 ClRCULAB NO. Ill, BUREAU OF PLANT INDUSTRY. 3 acres, while an equal area of Columbia produced less than 1 baJe. In L912 both fields were planted to Durango cotton, which produced an excellent crop on the same land where the Columbia had failed the year before. The behavior of the Columbia hi a near-by experimental planting was much the same in 1912 as in 1911. Even where the plants had ample exposure instead of field conditions the yield was altogether inferior to that of adjacent plants of the Durango cotton. (Fig" 2.) Fig. 1.— Durango cotton plant at El Centro, Cal., with leaves removed to show habits of branching. Comparisons between some of the best plants in the two rows showed over three times as many open bolls on the Durango plants and a general yield of about twice that of the Columbia. These contrasts appeared the more remarkable because the Columbia is a very pro- lific variety in the South Atlantic States. The differences of behavior can be understood when the habits of the two varieties are compared in detail. The failure of the Columbia cotton to develop a large crop of bolls under the Imperial Valley con- ditions is evidently connected with the tendency to produce a dense canopy of leaves. This is quite different from the behavior of the DURANGO CO!' TON l.\ 111 MI'KUIAI. VALLEY . \t variety in the Atlantic States, whore the plants are smaller and more prolific and have much more open foliage. When the foliage becomes too dense only the growing ends of the stalks and vegetative 1 branches have adequate exposure to the sun- light, while the fruiting branches are heavily shaded. Many of the buds are blasted and many of the bolls fail to reach normal maturity. Premature opening of the bolls and mildewing under the continuous shade also contribute to the inferiority of the fiber. There are also many plants with short lint, showing that degenerate variations have Fin. 2.— Columbia cotton plant (left) and Durango cotton plant (right), showing different habits of growth, at El Centro, Cal. The ( olumbia plant had 22 open bolls, the Durango plant 62. boon induced by the new conditions. Such degeneracy was especially noticeable among volunteer plants of the Columbia variety in the season of 1912. If the tendency to luxuriant growth and heavy foliage could be restrained by withholding water or planting on less fertile soils, somewhat more favorable results could doubtless be secured with Columbia cotton. But the Durango is much better adapted to the general conditions in the Imperial Valley, and there is no reason for making special efforts to grow the Columbia. The fact that the I oh-, mi 16 CIRCULAR NO. Ill, BUREAU OF PLANT INDUSTRY. Columbia variety is not well adapted to southwestern conditions does not affect its value in South Carolina and adjacent States, where it is now attaining a well-merited popularity. DTJRANG-O COTTON COMPARED WITH THE FOSTER. The Foster is a variety of long-staple Upland cotton bred by Dr. D. A. Saunders, of this department. It was developed from a hybrid between the Triumph variety of Texas big-boll cotton and Sunflower, a well-known long-staple Upland variety of the Peeler type. - Foster cotton is adapted to the Red River Valley of Louisiana and northeastern Texas, where it originated. It is an early, prolific variety, producing 1^-inch lint under favorable conditions, but shows a persistent tendency to occasional reversions toward the characters of the Triumph parent. It is on account of this tendency to variation that the Foster cotton has not been distributed more widely in the long-staple districts of Louisiana and Mississippi, though excellent results have been secured with many plantings. The behavior of Foster cotton in the Imperial Valley does not present the same difficulties as in the case of Columbia. The plants do not become very luxuriant or densely leafy, but show rather an opposite tendency to early maturity and dwarfing, which limit the yield. Thus, in an experimental-row planting at El Centro in 1912 even the largest of the Foster plants were scarcely half the size of the Columbia and Allen that stood on either side of the Foster row. (Fig. 3.) Some of the Foster plants were very prolific for their size, but the best of them produced less than a third as many bolls as the Durango. The yield was only a little more than half that of the Columbia or a quarter that of the Durango. (Fig. 4.) A few fields of Foster cotton were grown in the Imperial Valley in 1912 from seed secured from Mississippi under the name " Unknown." The seed seems to have been brought to the valley under the impres- sion that it represented the regular long-staple or Peeler cotton, in order to test the possibilities of this type of cotton in the Imperial Valley. But irr reality the behavior of the Foster cotton is radically different from that of a true Peeler cotton, such as the Allen, which stood next to the Foster in the experimental plantings made by the Department of Agriculture. In the fields of Foster, or so-called "Unknown," diversity was very apparent in the habits of growth of the plants and the sizes and shapes of the leaves and bolls. Lint samples showed the usual tendency to Triumphlike reversions, ren- dering the staple distinctly less uniform than in the Durango, though still of good, marketable quality. The yield also did not approach that of many of the Durango fields. [Cir. Ill] DURANGO COTTON IN THE IMPERIAL VALLEY. 1 < DURANGO COTTON COMPARED WITH THE ALLEN. The Allen variety has been included in experimental plantings as the best representative of the Peeler varieties which formerly fur- nished the long-staple crop of Mississippi and Louisiana. The results leave no doubt that in the ImperiaL Valley the Allen is a very un- promising variety, on account of the usually low yields of seed cotton, the low percentage of lint, and the small bolls. Even when the plants are large and vigorous they may yield very little (fig. 3). In all these particulars the Allen falls far below any of the other varieties included in the test. In the El Centro planting of 1912 the lint Fig. 3— Allen cotton plant (left) and Foster plant (right), showing different habits of growth at El Centro, Cal. The Allen plant had 12 open bolls, the Foster plant 14, in rows adjacent to those illustrated in figure 2. percentage was only 20, whereas none of the other varieties fell below 27 and the Durango gave over 30. With respect to the size of the bolls the Allen cotton has little or no advantage over the Egyptian varieties and is also less productive. If the Peeler cottons were the only Upland long-staple type available there would be no pros- pect of establishing a long-staple industry in the Imperial Valley, for planters would continue with short-staple cottons or go over to the Egyptian. Either of these alternatives would be more promising than the growling of the Peeler varieties. [Cir. Ill] 18 CIRCULAR NO. Ill, BUREAU OF PLANT INDUSTRY, DURANGO COTTON COMPARED WITH THE EGYPTIAN. On account of the present scarcity of Upland long-staple cotton the Durango cotton enjoys a somewhat artificial and perhaps tem- porary market advantage over the Egyptian. The scarcity of long- staple Upland cotton is due largely to the fact that the presence of the boll weevil interferes with the growing of the Peeler varieties in the former centers of production in Mississippi and Louisiana. Short-staple varieties have replaced the long staples in many dis- tricts, but efforts are now being made to go back to the cultivation of long staples on the basis of the new early varieties like the Colum- bia, Foster, and Durango. A part of the deficiency is being made good by the planting of Columbia and other early long-staple varie- t ies in the Piedmont region of the South Atlantic States, but it may be several years before an adequate supply of long staples can be developed in this region. Although the relative desirability of the Durango or of Egyptian cotton for the Imperial Valley and other irrigated regions of the Southwest is likely to fluctuate with the state of the market, it will be very poor policy for cotton- growing communities to make frequent changes from one type of cotton to the other. To learn the methods of grow- ing, selecting, handling, and marketing either type to the best advantage is likely to require several years, so that a change from one to the other must be a very expensive operation. Scarcity of labor and the higher cost of picking are local factors that now stand seriously in the way of developing community pro- duction of the Egyptian cotton in the Imperial Valley and make it appear preferable to adopt the Durango. Even though the individual farmer may not agree with his neighbors regarding the variety or type of cotton that should be grown, he is likely to gain more by growing the community variety than by producing a small quantity of some other kind of cotton that he is unable to sell to advantage. SPECIAL CHARACTERS OF DURANGO COTTON. In addition to the features to which reference has been made in the preceding paragraphs, several other characteristics of Durango cotton are w'orthy of mention. [Civ. 11 1 I Fig. 4.— Boll of Durango cotton, with involucre, showing relatively small bracts. (Natural size.) DURANGO COTTON IN THE IMPERIAL VALLEY. 19 One of the most peculiar characters is shown in the seedlings. In most other kinds of cotton the seedlings produce at least two or three simple oval leaves before beginning to develop leaves of the usual lobed form of the adult plant. But in Durango cotton a large pro- portion of the seedlings have lobes on all the leaves, including the first leaf above the seed leaves. This character might be of use in deter- mining early in the season whether any planting of Durango cotton is pure. The oidy other variety that has been found to have any considerable proportion of the first leaves lobed is the Alien. In Durango cotton the tendency to produce vegetative branches is less than in most other varieties, and it can be reduced still further by leaving the plants close together in the rows. Earlier crops and larger yields have been obtained in this way than by the usual method of thinning the plants early to 2 feet or upward. Some of the best yields have been obtained from plants only 6 or 8 inches apart in the rows. Another peculiarity of Durango cotton is the unusually small size of the involucral bracts. The difference in this respect between the Durango and the Triumph or other Texas big-boll varieties is very striking. The smaller bracts of the Durango cotton are a distinct advantage from the standpoint of clean picking, for the ends of the dry bracts are not so likely to be broken off and gathered with the fiber (fig. 4). Fragments of the involucral bracts furnish a large part of the "trash" that goes to the gin with the cotton and lowers the grade of the bale. The following formal description of the Durango variety has been supplied in connection with the congressional seed distribution: Plan! . Well-developed supernumerary carpels inclosing apparently normal seeds. From Clarksville, Tex. ( Nat- ural size.) 28 CIRCULAR NO. Ill, BUREAU OF PLANT INDUSTRY. tip of one of the carpels were that a threadlike projection extended beyond the normal carpels. Fertilization may have taken place through this extended tissue. It remains to be seen whether these seeds are capable of producing plants, but their presence in such a mature form proves most emphatically the real nature of these abnormalities. Mr. Clark's own description of the specimen found at Clarksville follows: A boll of an Upland variety of cotton found at Clarksville, Tex., in the fall of 1912 had developed between the placenta? two large supernumerary carpels which con- tained two apparently normal seeds. One of these abnormal carpels was thick and hard, resembling a normal carpel except that it was narrower (8 millimeters) and attached along its back to the placenta of a normal carpel for half the length of the normal boll. From here it projected independently. Although this projection had be'en broken off before the specimen was found, it must have extended to the apex of the boll and have furnished means of fertilization for the seeds. The other carpel had the appearance of a thin obovate leaf, measuring 1 centimeter in width and about 2 centimeters in length. This was attached only at the base. Three veins could be easily traced in this leaf-like carpel and the surface was dotted with oil glands, but it is doubtful whether placentge had been developed on the margins. Two locks were contained within the cavity formed by these two abnormal growths. One lock con- sisted of two apparently normal seeds and three rudimentary ovules. The other con- sisted of rudimentary ovules only. The two seeds, though a little undersized, pro- duced abundant lint, that of one measuring 33 millimeters and of the other, 25 milli- meters in length. The surrounding boll wa" in other respects normal, with the excep- tion that the five placentae of the carpels were flat and widened to about 3 millimeters. OCCURRENCE ON OTHER PLANTS. At Washington, D. C, on the grounds of the Department of Agri- culture, are several plants of althaea (Hibiscus syriacus) which in 1911 were abnormal in this same respect. Supernumerary carpels, two to five in number, develop between the placentae, although the normal series of carpels are not distorted. The central carpels in some cases contain rudimentary ovules. [Cir. Ill] KEEPING SOFT CUTTINGS ALIVE FOR LONG PERIODS. 1 By George W. Oliver, Plant Breeder and Propagator, Office of Foreign Seed and Plant Introduction. It has often been found desirable to bring soft or herbaceous plant cuttings from long distances, but the difficulty heretofore attending their transportation has been that the cuttings do not remain in good condition longer than a day or two. This difficulty has been removed by an exceedingly simple contrivance. Dormant hard-wooded cuttings and scions can be sent long dis- tances by mail, as was demonstrated a few years ago in a collection of scions and bud sticks forwarded to Mr. William S. Lyon, at that time in the service of the Government of the Philippine Islands. Not only did the material reach its destination in good condition, but some of it was repacked according to instructions and returned to Washington, where it was successfully grafted in the greenhouses of the Department of Agriculture. Soft or herbaceous cuttings, on the other hand, such as those of alfalfa, clover, and many other plants, can not be sent long dis- tances by mail or express, but they will survive a journey of six weeks in perfect condition if kept where they can be given light occasionally and attention is paid to supplying the water lost through evaporation. This treatment in the case of alfalfa and many other plants induces healthy root action during a journey of several weeks' duration. The apparatus for successfully bringing cuttings of herbaceous plants from distant places is of the simplest nature. The necessary articles are a small quantity of living sphagnum moss, two sheets of strong glass, 5 by 7 inches or larger, and some string. The cuttings should be prepared in much the same way as though intended to be placed in a propagating bed. Arrange the first layer of cuttings without too much crowding and with the upper surfaces of the leaves on the first piece of glass and on top of the cuttings, and place about 2 or 3 inches of living sphag- num moss evenly distributed over the cuttings. Place another layer of cuttings on top of this moss, with the under surfaces of the leaves next to the moss, so that all the available space will be covered, and i Issued Feb. l, 1913. [Cir. Ill | 29 30 CIRCULAR NO. Ill, BUREAU OF PLANT INDUSTRY. on top of this second layer of cuttings place the second piece of glass. Press down firmly, remove the moss which protrudes beyond the edges of the glass, and tie together with stout twine (fig. 1). Fig. 1.— Alfalfa cuttings arranged on dampened sphagnum moss and covered with glass. The package now consists of two pieces of glass, 2 inches of pressed sphagnum moss, and two layers of cuttings — one between each piece of glass and the moss. By keeping the moss moist and giving all the light possible (direct sunlight is best, and it does not raise the temperature of the moss to 'an appreciable extent beyond that of [Oir. Ill | KEEPING SOFT CUTTINGS ALIVE EOR LONG PERIODS. 31 the surrounding atmosphere), the cuttings are not in the least injured, provided the material is free from fungous troubles. If the journey is long enough — say, of four to six weeks' duration — cuttings such as those of clover, a^alfa, Dorycnium, lotus, and many other plants will have rooted freely while closely pressed against the glass. During the time of rooting no attention is re- quired beyond keeping the moss wet and exposing the cuttings to the light for a few hours each day. With the moss only slightly dampened, scions and bud sticks of rare plants keep a very long tune in good condition under the same treatment. [Cir. Ill J o U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 112. B. T. GALLOWAY, Chief of Bureau. MISCELLANEOUS PAPERS. Opportunities in Pecan Culture C. A. REED The Jonathan Fruit-Spot W. M. SCOTT and J. W. ROBERTS Egyptian Cotton as Affected by Soil Variations T. H. KEARNEY Relation of Stand to Yield in Hops . W. W. STOCKBERGER and JAMES THOMPSON Issued February 8, 1913. LIBRARY DRK ANICAL 'GARDEN WASHINGTON: GOVERNMENT PRINTING OFFICE. 1913. BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau , "William A. Taylor. Editor, J. E. Rockwell. Chief Clerk, James E. Jones. [Cir. 112] 2 OPPORTUNITIES IN PECAN CULTURE. 1 By C. A. Reed, Scientific Assistant in Pomology. INTRODUCTION. The demand for authentic information pertaining to pecan culture was never so great as at present. Much has been said in a speculative way regarding the commercial future of the industry, the suitability of various localities, the probable bearing age, the size of crops, their immunity or susceptibility to disease and insect pests, then* relation to the weather, and many other matters, but with the industry still in an experimental stage such statements have been necessarily based largely upon limited experience and are therefore subject to revision on short notice. The feature of the industry concerning which there is the greatest interest is its commercial future. This information can be gained only through a full knowledge of what it has cost to establish and maintain pecan orchards properly, what the crops of nuts have been, what prices have been realized, the influence of increased production upon future prices, and the possibility of increasing the market demand for the nuts. DIFFICULTY OF OBTAINING ACCURATE DATA. Although the planting of pecan trees was probably begun in the eighteenth century, or perhaps even earlier, their planting in orchard form is comparatively recent. Some pecan orchards are known to have been planted prior to 1880, but until about 1905 practically all consisted of seedling trees. In the planting of these early orchards little attention was paid to such matters as seed selection, the adapta- bility of locality and soil to the species, proper distances between trees, their cultivation, etc., the importance of which is now well understood. A few such seedling orchards when kept under culti- vation have begun to bear crops of more or less importance at 15 to 25 years of age, but few orchards have made records as to bearing which would be of interest even if obtainable. The chief value of such orchards is in the opportunity they afford of quick transforma- tion by top-working to named varieties. The growing of named varieties — trees propagated by budding and grafting with scions from individual parent trees — began to be active about 1890. At that time comparatively few varieties were known, 1 Issued Feb. 8, 1913. [C3r. 112.] 3 4 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. and during the next decade less than a dozen were widely dissemi- nated. Of these several proved unsatisfactory and have since been superseded. During the second decade of active interest in pecan orcharding a great many varieties were introduced, most of winch are already disappearing. The dissemination of new varieties, many of which may later prove to be of little importance, is still going on and may be expected to continue. Many of the disseminated varieties have been disappointing in that they have not fruited as was expected, especially in localities other than those where they originated, while some have proved highly susceptible to fungous diseases. By far the greater number of promising orchards of budded or grafted trees now existing are still too young to bear commercial crops. It is only here and there that orchards more than five or six years of age, of good varieties, well adapted to local conditions, and under a high state of cultivation are to be found. A number of orchards which might otherwise have been in bearing have been so heavily cut for bud wood that the chances of fruiting have been im- paired for the time being. Occasional individual tree records made under highly favorable conditions are frequently taken as a basis for estimates of what may be expected from orchards of the same varieties of the same age. The fallacy of taking these records as a basis for orchard estimates is apparent when it is realized that it is entirely impracticable to maintain in large orchards the garden conditions under which such records have usually been made. This has recently been emphasized by the record of an orchard of 200 trees in southwestern Georgia, the exact weight of the nuts from each tree for the last two seasons having been personally recorded by the writer during the harvest. In 1911, the seventh season from planting, the crop from these 200 trees, all of which are of one variety, of the same age, and under the same degree of cultivation, amounted to 1,137 pounds and ranged from a few nuts per tree, and occasionally none at all, to 17^ pounds in the case of one tree. The average for the entire orchard was 5.08 pounds per tree. In 1912 the total crop fell to 639 pounds, or an average of 3.19 pounds per tree, the range of individual trees being from no crop to 13^ pounds. If estimates of the probable yield of the entire orchard were to be based upon records of single trees in this orchard the figures of the total crop would show a range from no crop to 3,500 pounds during 1911 and 2,700 pounds during 1912, none of which would be correct. THE COST OF ESTABLISHING AND MAINTAINING PECAN ORCHARDS. A letter of inquiry regarding the most important items in the cost of establishing and maintaining pecan orchards was recently sent out from the Bureau of Plant Industry to a number of persons in the [Cir. 112] OPPORTUNITIES IN PECAN CULTURE. O growing districts who have had experience in orchard culture. Re- plies were received from 17 growers in the States of Georgia, Florida, Mississippi, Louisiana, and Texas. The questions asked and the replies made were both limited in number and brief, but as they brought out the opinions of some of the most prominent and succsssful pecan growers, the questions and a summary of the replies are here presented. (1) At about what price per acre can cleared land in your section suitable for pecan planting be purchased? The estimates made were mostly from $20 to $40 per acre; one was $40 to $100, one $75, and one $200. (2) At what price can uncleared land be obtained? One estimate was $5 to $15 per acre. Most were from $10 to $30. One was from $20 to $50, and one $125. (3) About what ought it to cost to put uncleared land into shape for planting? In some instances the estimates made included the cost of removing stumps, stating that frequently the value of the standing timber w T as sufficient to pay for clearing. The estimate for clearing, removal of the stumps included, ranged from $18.21 to $28.21 per acre. (4) About how much per acre has it annually cost you thus far for fertilizer in your pecan orchard? The kind and quantity of fertilizer pecan trees should receive vary greatly, depending upon local conditions. Some orchardists use no commercial fertilizer, relying entirely upon leguminous crops and stable manure. Others feed the trees by fertilizing the crops grown between the rows, while some rent the land between the rows, reserv- ing a strip along the row which they (the owners) cultivate and fertilize independent of the rest of the land. Estimates as to the actual cost of fertilizing the trees alone are very difficult to obtain. Some replies indicated that from $10 to $25 per acre was paid annu- ally for fertilizer for the land, including both that given the trees and the intercrops. Others showed that to apply 2 pounds of fertilizer to each tree during the season of its planting and to increase this by 1 pound per tree each year thereafter had cost thus far from $1.50 to $2 per acre. (5) Have you found the growing of other crops between the trees to be practicable; and, if so, about how much per acre have been the annual gross returns from such crops? (6) What crops have you found to be most practicable for growing between pecan trees? The invariable reply to these inquiries was in favor of growing intercrops. Cotton, corn, and leguminous crops were most com- monly recommended, although a few from truck-growing districts reported much better returns from vegetable production. One or [Cir. 112J 6 CIECULAR 112, BUREAU OF PLANT INDUSTRY. two reported very favorably on growing nursery stock. Some said that during the first five to seven years the land would be quite as valuable for cultivated crops as open land. It is obvious, however, that after the trees reach bearing age the value of the land between the rows for intercropping must depreciate rapidly. (7) Have you kept any record of the cost of cultivation? If so, about how much has it been per acre each year, including both the cultivation of the trees and the crops between the rows? With but one or two exceptions the replies to this inquiry were to the effect that no record had been kept. Such records as have been kept include the cost of cultivating the intercrops, and it was there- fore impossible to determine the separate cost of cultivating the trees. One letter stated that the annual cost of cultivating the trees would not be more than $1 per acre; another, that this cost would not exceed $5 per acre. The questions asked omitted a discussion of the price of the trees, the cost of planting, the salary of a supervisor, the cost of replant- ing dead trees, pruning, spraying, and harvesting the crop, and many other items which must be taken into account when a com- plete record of the cost of establishing and maintaining pecan orchards is undertaken. As it is customary to plant from 17 to 20 trees per acre, the cost of the trees can be quickly computed by consulting the nurserymen's price lists. The other factors are all either very vari- able or else few data are available. YIELDS THAT MAY BE REASONABLY EXPECTED. The gross returns which may be realized from an orchard at any given age depend upon the size of the crop and the price received. Considerable light upon the former may be obtained from a summary of yield records already made, and such records as it has been pos- sible to obtain are here included. The price which has been realized in the past will, of course, have some bearing upon what may be expected in the future, but it can not be taken as a safe criterion. In the past pecans of named varieties have been grown in very small quantities and have been largely utilized by nurserymen as samples and by fancy confectioners, tourists, and land sellers. In this way a high price has been maintained. Ordinarily the pro- ducers have received from 30 to 50 or 75 cents a pound for nuts of the best varieties. The demand for the best pecans can not become general until prices settle to a uniform level within reach of the con- sumer of average means. The present extensive planting insures abundant production, and the need of a wide market is therefore self-evident. With the disappearance of fancy prices, the general demand will undoubtedly materially increase. Further, the prices [Cir. 112] OPPORTUNITIES IN PECAN CULTURE. which the producer will realize in the future will depend more largely than in the past upon the prices of wild nuts, with which the culti- vated product must soon enter into competition. The present pre- vailing retail price for pecans from native trees is from 20 to 30 cents per pound, uncracked, and from 60 to 90 cents for half kernels. Estimates of yields that may be expected must be based upon records of orchards of the same varieties and age when under sub- stantially the same conditions of climate, soil fertility, moisture sup- ply, and cultivation. The difficulty in obtaining records upon which to base such estimates has already been shown. The figures pre- sented in Table I will serve for general guidance until more and better records are available. Table I.— Yield records of pecan orchards. W inter planted. Num- ber of trees. Average number of pounds per tree each season. Location and variety. 4th. 5th. 6th. 7th. 1 8th. 9th. 10th. 11th. 12th. Southern Georgia: Jewett, Rome, Stuart, [■1899-1900. 1901-2.... 1902-3.... •1904 250 [2 321 0.40 0.90 0.68 and Van Deman. Southern Mississippi : ' Delmas '0.44 0.81 5.23 12.15 Stuarl (*) m ( 4 ) ( 4 ) 0) .78 ( 4 ) .21 Pahst 70 70 12 70 70 292 1.43 3.21 2. H 3.97 5 1.55 3.28 3.28 7.71 5.28 .50 16. 42 6 3.21 7.84 Total .50 2.53 3.2S \ Mixed, Stuart, Schley, ?209 200 Delmas, and others. Southwestern Georgia : J 1904-5.... 1904-5.... 0.92 1.05 5.08 270 2 1 1 26 300 3.23 .20 . 12 Clark .00 .81 Total .08 .12 .66 3.13 ll905-6. . . . f 1,265 68 • 413 34S I 330 .23 .73 .09 .14 .06 Schley Stuart Total 2,424 1 1904-5.... .45 Northern Florida: Mixed, Frotscher, Egg- shell, Schley, Money- maker, and others. 120 .48 1 In southern Mississippi two Stuart trees planted in the winter of 1889-90 bore 250 pounds each in 1911; in 1912 the crop from these two trees fell materially. 2 This orchard was reported as having 300 young trees set in the spring seasons of 1902 and 1903 and about 7 old trees top-worked in 1902 and 1904. The old trees annually bear about three times as much as the same number of young trees. In order to get an average per tree of the trees of one age, the top-worked trees are counted as being equivalent to three times as many, or 21. young trees. The date of planting the 300 trees is taken as having been 1903. ' Owing to a very hard storm in the fall of 1906 there was no crop in 1907 or 1908, the fifth and sixth seasons from planting. * A few nuts. 5 Schley and Stuart not included, s Schley, Stuart, and Van Deman not included. » Mature trees 25 to 30 years old; averaged 38. 49 pounds in 1911 and 9.57 pounds in 1912. [Cir. 112] 8 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. The figures shown in Table I, with the exception of those regarding the orchard of 200 trees previously mentioned, the yields of which were recorded by the writer, have been made by careful orchard owners, who furnished the data to the Department of Agriculture. Another record, which has been submitted in different form from middle Georgia, is as follows: Stuart Few nuts fourth year; increase yearly to ninth year 2£ to 3 pounds. Mobile Few nuts third year; increase yearly to ninth year 5 to 15 pounds. Teche and Frotscher Few nuts fifth year; increase yearly to ninth year 2 to 5 pounds. Rome Few nuts eighth and ninth years. Capitol Few nuts fiflh year; increase yearly to ninth year 2 to 3 pounds. Senator Atlanta \ "No good.' Centennial CONCLUSIONS. Pecan orchards demand the same intelligent management as other orchards. It is sometimes held that being a native of the forest the tree not only needs no cultivation but will do better without it. This theory might be applied with equal reason to any fruit tree, and in answer it is only necessary to suggest a comparison between wild and cultivated apples, pears, oranges, or other fruits. The impres- sion that the pecan tree has no enemies in the way of insect pests or fungous diseases, that it is not subject to damage from drought, wet weather, or freezing temperatures, and that the nuts are in unlimited demand at a dollar or more a pound is at variance with the facts. The pecan is often subject to serious injury by numerous insects and diseases, and it is also much affected by unfavorable weather. Warm spells in winter followed by sharp freezes not infrequently result in the death of young trees; rains occasionally interfere with pollina- tion; prolonged dry weather causes the nuts to be small and perhaps to drop prematurely; and warm, wet weather may cause the nuts to become moldy or to germinate while still in the hulls. Although in the past choice pecans have frequently brought two, three, or more cents a nut when sold by the pound, it is evident that when sold on their merit in competition with wild pecans and when the orchards now being planted reach bearing age the prices will fall materially below the figures now often cited by enthusiastic exploiters. The prospective pecan grower should, of course, bear in mind that no horticultural product is free from its troubles, and while the pecan has its full share its culture probably has no more drawbacks than [Cir. 112] OPPORTUNITIES IN PECAN CULTURE. 9 other similar industries. Ordinarily, commercial returns are not to be expected until the trees are 10 to 12 years old. The length of time trees will continue to bear is a matter of conjecture. Commonly, old seedling trees are moderate bearers, the heavy bearers being fairly young or of middle age. The indications are that the most produc- tive trees of the forest are not long lived, and as the varieties selected and propagated for planting in orchards are usually heavy bearers it is not improbable that the shorter lived varieties are unconsciously selected at the same time. It is therefore not unlikely that the life of bearing pecan trees in orchard form will be much shorter than that of the average pecan tree of the forest. 76060°— Cir. 112—13 2 THE JONATHAN FRUIT-SPOT. 1 By W. M. Scott, Formerly Pathologist, and John W. Roberts, Assistant Pathologist, Fruit-Disease Invest igat ions . INTRODUCTION. In February, 1911, the senior writer 2 published a preliminary report on "A new fruit-spot of apple," 3 in which he stated that the cause of the disease was unknown, but that there was a strong suspicion of injury produced by arsenate of lead used in spraying. It was also stated that the fungi Cylindrosporium pomi Brooks and Alternaria sp. were isolated from a few of the spots, indicating a possible connection of one or both of these organisms with the disease. The results of spraying experiments and further laboratory studies conducted by the writers show that the spots are not due to arsenate of lead injury and probably are not caused by any vegetable organism. THE NATURE AND IMPORTANCE OF THE DISEASE. The spots, though seldom more than skin deep, detract greatly from the appearance of the apple and afford a place of entrance for decay fungi. They are dark brown in color, more or less circular in outline, at first scarcely depressed, later becoming considerably sunken, and vary from one-eighth to three-fourths of an inch in diameter. (Figs. 1 and 2.) They resemble very young bitter-rot spots and are not easily distinguished from the advanced stage of the New Hampsliire fruit-spot (Cylindrosporium pomi Brooks). As many as 25 spots often occur on one apple, and a lenticel usually forms the center of each spot. Since the spots are entirely super- ficial, the intrinsic value of the fruit is not seriously affected, but its market value is greatly reduced. The disease occurs only on fully matured fruit and usually develops after the crop is picked. If left on the trees long after maturing, the fruit of susceptible varieties may become affected before being picked. This was observed on the Jonathan variety in Virginia 1 Issued Feb. 8, 1913. ^ The work covered by this paper was done previous to the resignation of Mr. Scott, which occurred in February, 1912. 3 Phytopathology, vol. 1, no. 1, p. 32-34. [Cir. 112] 11 12 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. and West Virginia during the fall of 1911. According to numerous observations made by the writers, fruit picked at the proper time, or rather early, and rushed into cold storage with only two or three days' delay, and consumed within a few days after removal from storage, will not develop the disease to any serious extent. Fruit of susceptible varieties kept in common storage or delayed in reaching cold storage usually becomes affected. The disease has been par- ticularly annoying to fruit growers who have attempted to keep prize specimens of the Jonathan in cellar storage for exhibition purposes. The growers of Esopus (S pitzenberg) in Oregon and Washington Fig. 1.— Esopus (Spitzeriberg) apple showing early stages of the Jonathan fruit-spot. have perhaps suffered most from this trouble, the spots often develop- ing on the fruit en route to the eastern markets. The writers have observed large quantities of affected fruit from the Northwest in the markets of Washington and New York. The Jonathan is the most susceptible variety grown in the east, and its commercial standing is greatly impaired on account of this weakness. The disease is now rather commonly known among apple growers as the "Jonathan spot," and for that reason the writers have adopted the name "Jonathan fruit-spot." The Esopus is almost, if not quite, as susceptible to the disease as the Jonathan, and the Yellow Newton apparently ranks third in degree of suscepti- [Cir. 112] JONATHAN FRUIT-SPOT. 13 bility. It has also been observed to a very slight extent on the Grimes, Arkansas Black, and a few other varieties of less importance. Dry weather during the summer is apparently favorable to the de- velopment of the Jonathan fruit-spot. It was very bad in 1910 and 1911, both of which were dry seasons, while in 1912, a comparatively wet season, it was not common on eastern-grown fruit. In the fall of 1911 the spotting was particularly serious on the Jonathan, speci- mens having been received from practically every section of the country where that variety is grown. Fig. ?..— Esopus (S pitzenberg) apple showing older stages of the Jonathan fruit-spot. SPRAYING AND STORING EXPERIMENTS. In order to test the supposition that the Jonathan fruit-spot might be due to arsenical injury, spraying experiments were con- ducted in the orchard of Air. S. II. Derby, at Woodside, Del., during 1911. A block of Jonathan apple trees about 15 years old was divided into 5 plats of 6 trees each and treated as follows: Commercial lime-sulphur solution at the rate of \\ gallons to each 50 gallons of water was used in connection With arsenate of lead on all of lite sprayed plats. The amourjt of arsenate of lead was varied from one-half pound to 5 pounds in each 50 gallons of spray. Plat I was sprayed with one-half pound, Plat II with 1 pound, [Cir. 112] 14 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. Plat III with 2 pounds, and Plat IV with 5 pounds of arsenate of lead to each 50 gallons of the diluted lime-sulphur solution. Three applications were made in accordance with the usual directions for the control of the codling moth, i. e., (1) as soon as the petals fell, (2) three weeks later, and (3) ten weeks after the petals fell. The trees were thoroughly sprayed each time, so that the fruit remained coated with the arsenate of lead well on toward picking time. Plat V was left unsprayed as a check. The crop was picked on September 12 and found to be practically free from insects and diseases. No spotting was discernible at this time. Two boxes of fruit from each plat were immediately sliipped to Washington, reaching the laboratory on September 15, three days after picking. On this date a careful examination revealed no indication of the disease on any of the fruit, sprayed or unsprayed. One box from each of the five plats was then placed in cold storage, while the remaining five boxes were stored in a moderately cool basement. The basement-stored apples were examined on September 30 with the following results: The fruit from Plat I showed 41 per cent affected with the Jonathan fruit-spot, Plat II 52 per cent, Plat III 36 per cent, Plat IV 36 per cent, and Plat V (check) 46 per cent. A reexamination of the same apples on October 23 showed Plat I to have 56 per cent of the fruit affected, Plat II 70 per cent, Plat III 52 per cent, Plat IV 42 per cent, and Plat V (check) 64 per cent. Many of these apples were seriously injured, being literally covered with spots measuring from 5 mm. to 1 cm. in diameter. These results show that unsprayed fruit may become quite as badly affected with the Jonathan fruit-spot as fruit sprayed with arsenate of lead. An unusually heavy dose of the poison, as shown in the results from Plat IV, which was sprayed with 5 pounds of arsenate of lead to 50 grallons of water, did not increase the amount of affected fruit. The fruit which was placed in cold storage was examined on November 10 and all of it found to be free from the disease. Finally, on December 18 these apples were removed from cold storage and examined with the following results: Plat I had 5 per cent of its fruit spotted, Plat II 10 per cent, Plat III 20 per cent, Plat IV 14 per cent, and Plat V (check) 33 per cent. In most cases the spots were small, inconspicuous, and few to an apple, being in these respects in great contrast to those appearing on the basement-stored fruit. The cold storage prevented the spotting for at least two months, and at the end of nearly three months tins fruit was not nearly so much affected as the cellar-stored fruit was at the end of six weeks. On September 25, 1911, one bushel of unsprayed and one bushel of sprayed Jonathan apples were received from Watervliet, Mich. These were sent in by Mr. E. W. Scott, of the Bureau of Entomology; they were taken from an orchard in which that bureau was conducting spray- [Cir. 112] JONATHAN FRUIT-SPOT. 15 ing experiments. The plat from which the sprayed fruit was taken had received the tliree usual codling-moth applications, arsenate of lead at the rate of 2 pounds to each 50 gallons of water having been used. Upon arrival an examination of this fruit failed to disclose any of the spot disease in either lot. Both lots were covered over in baskets and left in the laboratory at room temperature and reexamined on September 29. At this time characteristic spots averaging 5 mm. in diameter and from 1 to 25 to each apple were found on 9 per cent of the unsprayed and on 18 per cent of the sprayed fruit. On October 23, 65 per cent of the unsprayed fruit was found to be spotted and 66 per cent of the sprayed fruit was similarly affected. One can only conclude from the results of these experiments that spraying with arsenate of lead is not in any way responsible for the Jonathan fruit-spot. The spots develop on unsprayed fruit as readily as on that which has been thoroughly sprayed with arsenate of lead. It is evident that tins poison neither favors nor retards the develop- ment of the disease. LABORATORY STUDIES. Nearly 400 cultures of the diseased spots have been made in various ways and on various media, but no organism has been isolated with any degree of consistency. A species of Alternaria often occurred in cultures from fruit grown in the eastern part of the country, but cultures from northwestern-grown fruit were almost entirely barren. A few apparently successful inoculations were made by spraying Alternaria spores on Jonathans kept in moist chambers and the fungus reisolated, but both the Jonathan and Esopus (Spitzenberg) are so susceptible to the disease that they are apt to become spotted under any conditions outside of cold storage. In some cases both the inoculated fruit and the controls contracted the disease at about the same time. Spores of this fungus inserted through needle punctures failed to produce the disease. As Alternaria is very commonly associated with the rotting of apples, especially when the fruit is placed in cellar or basement storage, the possibility of its being the cause of this disease becomes very remote. The fungus Cylindrosporium pomi Brooks occurred in a few of the cultures, but this was probably accidental. It is not unlikely that the Brooks spot and the Jonathan fruit-spot occurred together on some of the apples from which cultures were made, and for this reason the fungus causing the former might easily have found its way into a few of the cultures, particularly since the two spots are somewhat similar in appearance. Cultures from the true Brooks spot produced the fungus readily, while those from the Jonathan fruit-spot were, with few exceptions, barren. Moreover, spraying [Cir. 112] 16 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. with a fungicide prevents the former disease, but has no effect upon the latter. It seems evident, therefore, that these two diseases are distinct. Microscopic examinations of the affected tissues failed to reveal the presence of any organism to which the disease could be attributed. The cells involved resemble similarly located cells in cases of "bitter- pit," or "Baldwin spot," in which that disease extends to the surface of the apple. Bitter-pit differs from this disease, how- ever, in that it is essentially a disease of the fleshy portion of the fruit, often reaching to the core without affecting the skin, while the "Jonathan spot" is usually little more than skin deep. The writers consider the disease a physiological one, but as in the case of the bitter-pit the cause is at present obscure. SUMMARY OF CONCLUSIONS. The investigations conducted by the writers seem to warrant the following conclusions : (1) The Jonathan fruit-spot of the apple is due neither to spraying with arsenate of lead nor to a specific organism. (2) It is probably a physiological trouble, falling in the same category as the bitter-pit or Baldwin spot. (3) Early picking, prompt cold storage, and immediate consump- tion of the fruit after removal from storage, will largely obviate losses from the disease. [Cir. 112] EGYPTIAN COTTON AS AFFECTED BY SOIL VARIATIONS. 1 By Thomas H. Kearney, Physiologist in Change of Alkali and Drought Resistant Plant Investigations. INTRODUCTION. Observation of the growth of Egyptian cotton in irrigated soils of the southwestern United States for several years past has shown that this plant is decidedly sensitive to variations in its physical environ- ment, Differences in the texture, and consequently in the moisture- holding capacity of the soil, are easily detected from the accompany- ing differences in the size, appearance, and f ruitfulness of the plants, in the size of the bolls, and in the quality of the fiber. The presence of alkali salts in the soil also induces marked differences in the growth and behavior of the cotton plants. It is evident that in order to obtain the largest yields and, what is of the utmost importance, the greatest possible uniformity in the staple, strength, and other quali- ties of the fiber, Egyptian cotton must be grown in soils that do not vary greatly in texture and salt content. MOISTURE CAPACITY OF THE SOIL. In many plantings of Egyptian cotton which have been made on recently cleared land the fields have appeared more or less spotted, the plants in some places being smaller, more erect, and lighter col- ored, with fewer and smaller bolls and shorter, often weaker, fiber than in other places. Marked differences of this kind frequently appear within distances of a few feet. A field of the Yuma variety on the United States Experiment Farm at Bard, Cal., in 1911 showed conspicuous local differences in the growth and appearance of the plants. Soil samples were therefore collected at a number of differ- ent points corresponding to various stages in the size and condition of the plants. Upon making the borings it was at once evident that the variations in growth of the plants were closely correlated with variations in the depth of the blanket of silt loam which overlaid a subsoil of coarse, light-colored sand. The depth of the silty layer varied from 5 to 18 inches in different parts of the field. Where it was thinnest the plants were poorest, and vice versa. The moisture-holding capacity of these two soils was widely dif- ferent, that of the silt loam being high, as indicated by a moisture i Issued Feb. 8, 1913. 17 18 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. equivalent, 1 in samples from different parts of the field of 24.4 to 31.4 per cent, corresponding to a wilting coefficient of 13.3 to 17 per cent. The coarse sand had a moisture equivalent of only 2.8 per cent (wilting coefficient, 1.5 per cent). The salt content of the soil in the different samples was investigated by means of the electrolytic bridge and was found to be nowhere sufficiently high to indicate that alkali was a factor in bringing about these differences of growth. The case was clearly one of the presence or absence of a sufficient depth of soil having a high enough water-holding capacity to prevent the plants from suffering as a result of drought between irrigations. It is rather surprising, in view of the very low water capacity of the underlying sand, that a depth of the silty layer of only 14 or 15 inches should have been sufficient to enable the plants to make a strong growth and produce numerous large bolls. The results of this investigation are summed up in Table I. Table I. — Relations between the depth of silt and the growth of Egyptian cotton at Bard, Cat., in 1911. Boring No. Depth of silt loam overlying coarse sand. 1 Condition of the plants. 6 1 5 2 4 3 7 8 Inches. 2 18 17 14 to 15 12 11 9 3 7 to 8 5 Rank growing, dark green, very fruitful, bolls large; growth here is heaviest in field. Good sized, dark green, fruitful. Similar to those at boring No. G. Smaller, lighter green, and less fruitful than at boring No. 1. Small, erect, light colored, with few small bolls and inferior fiber. Similar to those at boring No. 4. Similar to those at borings Nos. 3 and 4. Poorest in the field, only 3J feet high, erect, light colored; bolls very few and very small, fiber very inferior. 1 Unless otherwise specified, the silt rested directly upon the rather coarse, light-colored sand. This sand extended to a depth of at least 4 feet at every boring which was carried to that depth (Nos. 1, 3, 5, and G). 2 Here the silt was underlain by 15 inches of a fine, reddish-colored sand (moisture equivalent, 7.6 per cent), which in turn rested upon the above-described coarse sand. 3 Here the surface silt was of lighter texture than elsewhere in the field (moisture equivalent, 24.4 per cent). It rested upon G to 7 inches of very fine sand, which was in turn underlain by the coarser sand above described. In 1912 similar observations were made on the same farm. A series of soil samples were taken midway between two rows of Egyptian cotton, in one of which the plants had been thinned to a distance of 6 inches and in the other to a distance of 18 inches. 2 Borings were made at three points: (1) Where the plants were tall, luxuriant, and dark green in color; (2) where the plants were smaller and of a light yellowish green color; and (3) where the plants were 1 The term "moisture equivalent" is defined and the value of this factor as a measure of the moisture- holding capacity of the soil is pointed out by Briggs and Me Lane in Bulletin 45 of the Bureau of Soils (1907). The moisture equivalent of a given soil being known, the wilting coefficient for plants growing in that soil can be calculated by means of the formula given by Briggs and Shantz in Bulletin 230 of the Bureau of Plant Industry, p. 58 (1912). All determinations of moisture equivalent referred to in this paper were made by Mr. J. W. McLane, of the Biophysical Laboratory, Bureau of Plant Industry. 2 The field had been planted under the direction of Mr. O. F. Cook in order to study the effect of dif- ferent thicknesses of stands upon the development of the vegetative branches. [Cir. 112] EGYPTIAN COTTON AS AFFECTED BY SOIL VARIATIONS. 19 small and yellowish green. Each of the three samples consisted of three cores taken about 1 foot apart and to a depth of 4 feet. The soil from all three cores at each successive 1-foot depth was thoroughly mixed together to represent that depth of the boring in question, and upon each of the 12 samples as thus prepared four determina- tions of moisture equivalent were made. The electrical resistance of saturated soil from each sample was measured in order to ascertain whether there were significant differ- ences in the salt content of the soil. The high resistances observed in every case made it evident that the effects noted could not be attributed to alkali. On the other hand, the fact that the resistances were much lower at all depths of boring No. 1 than of borings Nos. 2 and 3 indicated that a deficiency of nutrient salts at the two latter borings may have been a factor in the poor growth of the plants. On several plants in the neighborhood of each boring counts were made of the number of the node on the axis at which the first fruiting branch was retained and of the number of set bolls to the plant. 1 Table II gives for each of the three borings the wilting coefficient (calculated from the moisture equivalent) and the electrical resist- ance of the soil at successive depths, as well as the average height of the plants, the mean of the numbers of the node bearing the first fruiting branch, and the mean number of bolls per plant. Table II. — Relations between the wilting coefficient of the soil and the growth and fruitfulness of Egyptian cotton plants at Bard. Cal., in 1912. Soil. Plants. Boring No. Depth. Willing coefficient calculated from the moisture equiva- lent. 2 Electrical resistance of the saturated soil. Spacing. Number of plants on which counts were made. Color of foliage. Height. Mean number of node of first fruiting branch. Mean number of bolls per plant. 1 Feet. 1 ! 1 i 1 § Per cent. 11.7 8.2 4.5 1.6 2.8 2.0 1.5 1.2 1.8 1.2 1.3 1.2 Ohms. 427 271 460 968 1,370 1,318 2,108 Inches. 6 7 Dark green Feet. 8 15.1 65.4 18 4 •Dark green 8 17.2 78.0 2 6 11 Light green . . . 4.5 18.5 21.2 18 5 Light green . . . 4.5 17.0 38.0 3 1,353 2,164 2.043 2.164 6 7 Light green . . . 2.5 15.4 10.6 18 5 Light green... 2.5 15.2 15.0 < The counting was done by Mr. Rowland M. Meade. The importance of retention of the fruiting branches at low nodes on the axis as an indication of fruitfulness is pointed out by Mr. Argyle McLachlan in Bulletin 249, Bureau of Plant Industry, 1912, entitled "The branching habits of Egyptian cotton." 2 Based upon thoroughly mixed samples of each 1-foot depth, regardless of variations of texture within that depth. The variations are indicated by the following notes: Boring No. 1. Soil silty from the surface to a depth of 14 to 20 inches (varying in the several cores), then fine sand, with some admixture of silt at a depth of 24 to 30 inches, then coarse sand from the depth of 30 to 48 inches. Boring No. 2. First 12 inches much sandier than at boring No. 1. Coarse sand began at a depth of 20 to 24 inches and continued to the bottom of the boring (48 inches). Boring No. 3. Soil sandy to the surface. Coarse sand began at a depth of about 18 inches and continued to the bottom of the boring. [Cir. 112] 20 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. These results confirm those of the previous year as to the effect of differences of soil texture upon the size and vigor of the plants, and also give a more definite expression of the effect upon the fruitfulness (number of bolls). There was apparently no consistent effect upon the height of the first fruiting branch. It is evident, therefore, that in the absence of appreciable quantities of alkali salts, the size, vigor, and fruitfulness of Egyptian cotton plants in alluvial soils along the Colorado River is largely determined by the depth of the layer of silt with its relatively high moisture capacity and its doubtless greater supply of plant food. The very sandy soils have so low a moisture capacity that they hold very little water even immediately after an irrigation and with the customary intervals between irrigations they soon become so dry that much of the time the plants are without available water. This frequent condition of virtual drought seriously impairs the yield and quantity of fiber produced in such soils. ALKALI CONTENT OF THE SOIL. In a previous publication l the results of observations on the growth of Egyptian cotton plants hi alkali soil at Sacaton, on the Gila River, hi southern Arizona, were summed up as follows: No plants grew at Sacaton in places where the average amount of alkali in the first 3 feet of soil was as high as 1.7 per cent. While resistant individual plants can pro- duce a small amount of fairly good fiber in the presence of from one-half to 1 per cent of alkali, it is probable that land containing considerably less than one-half of 1 per cent must be selected in order to obtain anything like a full stand and the best quality of fiber. The actual limit of safety remains to be determined. Since this statement was written, further observations upon the alkali resistance of this plant were made at Sacaton, Ariz., hi 1910, and at Bard, Cal., hi 1911. Even with these additional data the quantity of alkali of a given composition which limits the successful growing of Egyptian cotton can be stated only approximately. 2 In a general way, however, the more recent observations confirm the conclusion previously readied that Egyptian cotton is superior to many other crop plants in its ability to endure an excess of salts hi the soil. OBSERVATIONS AT SACATON, ARIZ. 3 A portion of one of the fields planted to the Yuma variety of Egyptian cotton was located where the soil contained more or less salts and where as a consequence the stand was very irregular. The i Circular 29, Bureau of Plant Industry, entitled "Experiments with Egyptian cotton in 1908," 1909, p. 18. 2 A discussion of the varying factors which make it difficult to determine in the field the limits for the growth of a particular plant in the presence of alkali will be found in Farmers' Bulletin 446, entitled " The choice of crops for alkali land," 1911, pp. 8 to 12. 3 These were made in October, 1910, at a time when the cotton had begun to ripen in most parte of the field. [Cir. 112] EGYPTIAN COTTON AS AFFECTED BY SOIL VARIATIONS. 21 cotton rows in this field began in good soil where the growth of the plants was normal and where the yield and character of the fiber were as good as could be expected considering the late date of planting (April 12). Toward the ends of the rows where the soil contained gradually increasing quantities of alkali salts the plants were small, produced few bolls, and ripened very late. Finally no plants remained where the quantity of alkali was excessive. In two of these rows the soil was sampled to the depth of 3 feet at the base of every tenth plant from the good end of the row until the region was reached where the stand was much interrupted. There samples were taken at more frequent intervals and finally alongside each remaining plant. The soil from each 3-foot boring was thoroughly mixed and its electrical resistance when saturated was determined by means of the electrical bridge, the standard container of which has a capacity of about 50 cubic centimeters. 1 From the resistances, the percentage of total salts to dry weight of soil were computed by means of a special correlation curve for the Sacaton type of alkali. This curve was based upon determinations of the electrical resistance and of the total water-soluble salts hi 48 samples of soil which had been col- lected two years previously on the experiment farm at Sacaton. Analysis of these samples hi the chemical laboratory of the Bureau of Soils showed the average composition of the Sacaton alkali to be as follows: '-' Per cent. I'er cent. Ca 1.0 Mg 3 K 1.0 Na 31.9 CI 24.4 S0 4 16:4 HC0 3 22.1 C0 3 5.0 Notes were made on the condition of each plant where a soil sample was taken and the seed cotton was collected for examination in the laboratory. Mr. Argyle McLachlan made a series of diagrams for each of these plants, showing graphically the number and position of the fruiting branches and the number of developed and aborted bolls on each branch. Comparison of these diagrams indicated that 1 The bridge is described and figured and directions are given for its use in determining the salt content of soils in Bureau of Soils Bulletins 15 (by L. J. Briggs) and 01 (by R. O. E. Davis and H. Bryan). In the latter publication tables are given for temperature correction (pp. 22 to 24) and for computing from the resistance the percentage of total salts to dry weight of the soil (pp. 14 to 16). 2 This composition of the alkali, and especially the presence of carbonates and of large quantities of bicarbonates, probably explains the fact that the Sacaton curve does not agree with that on which is based Table III, p. 14, in Bulletin 61 of the Bureau of Soils. The latter applies to alkali consisting of one-half chlorids and one-half sulphates. For resistances below 4() ohms it indicates higher percentages of total salts and for resistances above 40 ohms it indicates lower percentages than does the Sacaton curve. The disparity increases until a resistance of 170 ohms indicates twice as much total salts on the Sacaton curve as on the chlorid-sulphate curve. On the other hand, the curve for carbonates (" black alkali") on which is based Table VI, on p. 10, Bulletin 61 of the Bureau of Soils, indicates much higher percentages of total salts for given resistances than on either of the other curves until a resistance of 170 ohms is reached, from which point the Sacaton curve agrees very closely with the carbonate curve. [Cir. 112] 22 CIKCULAE 112, BUREAU OF PLANT INDUSTRY. there was a tendency for the plants growing in soil relatively free from alkali to retain the first fruiting branch at a lower node on the axis than in the case of plants in the stronger alkali soils, although the correlation between the height of the first fruiting branch and the alkali content of the soil was not a close one. At most of the borings the resistance of the saturated soil ranged from 200 to 400 ohms. Where it exceeded 200 ohms no differences which were not well within the limits of individual fluctuation could be detected in the plants. Table III summarizes the notes made chiefly upon plants growing where the soil gave resistances lower than 200 ohms. Table III.— Electrical resistance of saturated soil, indicated percentage of total salts, and character of the plants ami fiber of Egyptian cotton at Sacaton, Ariz., in 1910. Soil (averages for the 4-foot column). Plants. Electri- cal resist- ance in ohms. Indicated percent- age of to- tal salts (Sacaton curve). Size, earliness, and fruit fulness. Number of the node on axis where the first re- tained fruiting branch occurs. Bolls. Fiber. +200 200 185 155 140 110 100 90 70 -0.34 .34 .36 .41 .43 .48 .51 .54 .63 10 to 16 12 to 17 13 to 15 16 to 17 12 18 16 17 18 Normal Normal. do do Do. Ripening somewhat retarded, otherwise normal. Smaller and later ripening do Do. Normal or rather small. Small or medium. . Small Rather short, other- wise normal. Good in length, Late ripening, fairly produc- tive. Small, late ripening, with few bolls. do strength, and quality . Normal. ..do Short, but strong Very small Fair sized and fine. Normal. None matured. The data given in Table III indicate that with alkali of the Sacaton type, where the salt content of the soil exceeds 0.4 per cent of its dry weight (electrical resistance 150 ohms or lower), the fruitfulness of the plants is likely to be impaired and the ripening of the bolls seriously retarded. This would seem to be about the limit for profit- able production of this crop in the presence of alkali of the Sacaton type, although it is evident that the quality of the fiber does not necessarily suffer in the presence of 0.55 per cent of salts (electrical resistance of 90 ohms). OBSERVATIONS AT BARD, CAL. A small field of Egyptian cotton grown in the vicinity of Bard in 1911 was located on a sandy soil containing in spots so much alkali that the resulting stand of cotton was very uneven. There [Cir. 112] EGYPTIAN COTTON AS AFFECTED BY SOIL VARIATIONS. 23 were several areas of greater or less size where the plants either had failed to appear or had subsequently died. The electrical resistance of the soil was determined in different parts of this field on October 23, and notes were made upon the character of the plants where the respective soil samples were taken. The results of these observa- tions are summarized in Table IV. The percentages of total salts indicated by the electrical resistances as given in this table are computed from Table III, page 14, Bulletin 61 of the Bureau of Soils, which applies to a type of alkali consisting of one-half chlorids and one-half sulphates. Table IV. — Electrical resistance of the saturated soil, indicated percentage of total salts, and condition of Egyptian cotton plants, at Bard, Cal., in 1911. Bor- ing No. Depth. Character of soil. Elec- trical resist- ance. Indicated total salts in percent- age of dry weight soil. Condition of plants. 1 Fat. i 1 2 3 4 Average. Sandy loam... do ...'..do Fine sandy loam. Ohms. 45 (53 113 117 0.75 . 53 .29 .28 No plants; boring in center of a small bare spot. .46 { I i 1 2 3 4 Average. Sandy loam... do Sandv loam... do Fine sandy loam. do 33 63 63 91 62 59 .' 1.12 .53 1 Between two good -sized, healthy, fruitful plants bear- > ing abundant fiber of excellent quality, strong, 1| 1 inches long. 3 .53 .37 .53 .56 Among small scattered plants (less than half normal height) mostly dead. Plants shallow-rooted here. .50 1 2 3 4 A verage. Sandj' loam. . . do Sand 55 50 69 85 .60 .67 .48 .39 Between two good-sized, healthy, fruitful plants bear- 4 do > ing strong, abundant, silky fiber. Root system comparatively shallow. .53 .60 .41 .45 .34 5 1 2 3 4 Average Sandv loam. . . do do Sand 55 81 75 97 Beside a dying plant at edge of spot in center of which boring No. 3 was made. This plant bore a few open bolls, the fiber in which was coarse and weak. .45 Inspection of this table shows no close relation between the salt content of the soil and the growth of the cotton plants. Thus, at boring No. 2, located midway between two plants which were about as good as any in the field, the first foot of the soil contained con- siderably more soluble salts than the first foot of borings Nos. 1, 3, and 5, where there were either no plants at all or the plants were evi- dently suffering. 1 1 Much of this alkali had doubt less accumulated in the upper soil after the cotton was planted, the groimd water table in this field having reached the surface of the soil during the high-water stage of the Colorado River in June. It had lowered by the date when these borings were made, saturated soil having been encountered at a depth of about 4i feet in the neighborhood of boring No. 3. [Cir. 112] 24 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. The only conclusion that may safely be drawn from the foregoing data is that when other conditions are favorable plants of Egyptian cotton can remain hi good condition and produce numerous bolls and strong, abundant fiber of good length and quality where the soil to a depth of 4 feet contains as much as one-half of 1 per cent of water-soluble salts of this composition. The apparent ability of Egyptian cotton plants to withstand more alkali at Bard than at Sacaton is perhaps partly to be ascribed to accumulation of the alkali in the upper soil at the former locality after the plants had reached an advanced stage of growth. The different composition of the salts at the two localities is doubtless also partly responsible for the difference. The average composition of the alkali on the experiment farm at Sacaton is given on page 2 1 . The alkali in the field at Bard where the above-described observations were made had the following average composition: Per cent. Ca 8.4 Mg 4. K (included with Na). Na 37.1 Per cent. CI 33.0 S0 4 21.0 HC0 3 •---. 15-6 C0 3 None. At Bard, none of the very injurious free carbonates (" black alkali") was detected, while considerable quantities were present in some of the Sacaton samples. Moreover, there was a much higher proportion of lime (Ca) in the Bard samples, and this substance, as is well known, is very effective in neutralizing the poisonous effects of the sodium salts which form the bulk of the alkali at both localities. CONCLUSIONS. The moisture capacity of the soil is an important factor hi deter- mining the size, vigor, and fruitfulness of Egyptian cotton plants. A larger supply of nutrient salts in the heavier soils is probably also a factor. With irrigation as ordinarily practiced hi the Southwest, very sandy soils, having a low moisture capacity, are unsuited to this crop, since the plants are exposed to virtual drought during much of the period between irrigations. Recurring deficiencies of available water hi the soil are very unfavorable to the yield and quality of the fiber. New land as a rule should be avoided hi growing Egyptian cotton, as the soil commonly varies greatly in moisture capacity and the crop produced will be correspondingly lacking hi uniformity. The alkali resistance of Egyptian cotton is relatively high when other conditions are favorable. It would appear that fair yields of fiber of good commercial quality can be obtained where nearly one- half of 1 per cent of the total dry weight of the soil consists of readily soluble alkali salts, provided that carbonates ("black alkali") are absent or form only an inconsiderable proportion of the total alkali. [Cir. 112] RELATION OF STAND TO YIELD IN HOPS. 1 By W. W. Stockberger, Physiologist, and James Thompson, Scientific Assistant, Office of Drug-Plant, Poisonous-Plant, Physiological, and Fermentation Inves- tigations. INTRODUCTION. Among many hop growers the impression prevails that the average yield of hops per acre is annually growing less and that the produc- tivity of a large proportion of the hop soils is decreasing. The statistical data on this point, however, are so meager that it seems unwise to draw from this source very definite conclusions regarding the increase or decrease in yield per acre. From the records of the United States Census the average yield per acre of hops can be determined only at 10-year intervals throughout a period of 30 years, and since the figures for any given year will vary widely, depending on whether a light or a heavy crop is produced, it is mani- festly unsafe to assume that the averages for the census years neces- sarily represent actual conditions for the intervening years. If records of the average yield were available for each one of the 30 years the evidence of the figures might be accepted as a fair indica- tion of the general trend of the yield of this crop. The average yield per acre is materially influenced by a number of factors, prominent among which are seasonal conditions, soil type, and cultural methods. In case large areas are under consideration, such as a county or State, extensive changes in acreage or a shifting of the area of production may also materially affect the average yield. When such modifications take place, changes in the average yield reported for the given area have little bearing on the question of diminishing crop yields. Nevertheless, the statistical data on the average yield per acre in the several hop-growing States and in the chief hop-producing counties therein are worthy of careful considera- tion by every grower of hops, but it is of far greater importance that he should be fully informed as to the successive yields of his own fields. On certain types of soil not so well adapted to hops as the richer alluvial soils there is ample evidence of a declining yield, due funda- mentally to soil conditions. This decline is most noticeable in hop- yards located on uplands where beneath the shallow surface soil i Issued Feb. 8, 1913. [Cir. 112] 25 26 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. there is a layer of hardpan and clay. On the other hand, it is far from clear that the diminished output per acre reported for some of the rich, deep alluvial soils is due to their decreased productiveness, especially if such soils are overflowed in winter and thereby receive a deposit of sediment. The fact that the application of commercial fertilizers to some of these soils has as a rule yielded negative results seems to indicate that they are not lacking in available plant food, and a study of the other factors concerned will probably reveal the most important causes of the decline in yield, if such is actually taking place. It is the purpose of the present publication to direct attention to the often unappreciated extent of the losses due to imperfect stand and to offer certain suggestions which, if followed, should result in an increased yield without materially increasing the cost of pro- duction. CAUSES OF IMPERFECT STANDS. In newly planted yards a small percentage of missing hills may normally be expected, owing to the failure of some of the cuttings to strike root. In most cases, after a yard has come into full bearing the stand tends to become poorer and poorer through the dying out of the plants from causes at present imperfectly understood. This dying out occurs in all the hop-growing sections of the United States, but it is far more prevalent in some districts than in others. Many ingenious explanations have been offered to account for this trouble, but a satisfactory one yet remains to be found. From extensive observations made in the hop fields of the United States and of Europe the writers have reached the tentative conclusion that a primary cause lies in too severe or faulty pruning, in the bruising of the roots in plowing, and in the crushing of the crown of the plant by the feet of horses and the wheels of wagons when teams are driven over the fields. Hills often die out because of weakness or disease induced either by the rough treatment received when they are uncovered at pruning time or by the injuries inflicted by the plow or other implements used in cultivation. When the roots are bruised or torn they heal slowly and imperfectly and are almost certain to become infected by some of the destructive organisms widely distributed in the soil. In most hopyards some attention is given each year to replanting the missing hills, but, since the trouble is rarely taken to make certain that the cuttings are sound and vigorous and that they come from hills selected for their thriftiness and high yield, many replants either die outright the first year or maintain a struggling and un- profitable existence. The vigor of the cuttings is often impaired through the lack of precaution to keep them from drying out before [Cir. 112] RELATION OF STAND TO YIELD IN HOPS. 27 they are set, or the replanting is deferred until the soil has become so dry that it does not afford the conditions essential to proper growth. Replants usually make a poor growth unless the site of the missing hill which they are intended to replace is excavated, the dead crown and roots removed, and the soil replaced by fresh earth taken from midway between the rows. The stand may become imperfect through numerous other causes, but the ones here described should receive first consideration, since it is within the power of the hop grower to minimize in great measure their effect. VARIATION IN THE PERCENTAGE OF PERFECT STAND. The percentage of perfect stand varies widely and is to a large extent dependent upon the local conditions affecting a given hop field and upon the knowledge, skill, and industry of the hop grower. In some yards which have come under the writers' observation a careful count of the missing hills showed the stand to be 99.3 per cent, while in other yards the stand was found to be as low as 75 per cent. These, of course, represent extreme cases and are far less numerous than those in which the stand ranges from 90 to 95 per cent for indi- vidual fields. The percentage of stand for any given yard will be found to fluctuate from year to year, according to the rate at which the hills are dying out and the care and attention given to replanting. The estimate by inspection of the number of missing hills and the percentage of stand have been found to be very misleading. In every case in which a grower's estimate of the percentage of stand has been verified by an actual count of the missing hills, his estimate has proved too high, and it is believed that growers often deceive them- selves as to the extent of the loss suffered through an imperfect stand. An estimate of the percentage of stand that is based on a count of the missing hills in every fifth or tenth row, although less accurate than a full count, is much to be preferred to one based on inspection alone. VARIATION IN STAND ON A SINGLE ACRE. An exact record of the stand on an acre for 4 consecutive years shows some striking variations which are believed to be fairly repre- sentative of the conditions existing in many hopyards. This acre was laid off at one side of a large field which had been under hops continuously for 10 years, and during the 4 years it was under obser- vation it received the same attention and culture treatment as the remainder of the field of which it forms a part. At harvest time each year a record was made of the condition of each hill, and the position of each hill that was missing or which had vines bearing no hops was noted on a chart. From this chart the data in Table I were compiled. [Cir. 112] 28 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. Table I. — Comparison of the stand of hop plants on 1 acre for the years 1909 to 1912, inclusive. Factors of variation. Productive hills Missing hills Hills having vines with no hops Hills having " bastard " vines Hills having male vines Total Stand per eenl Productive stand do. . 1909 1910 1911 1912 853 56 43 5 10 865 66 21 10 5 887 24 50 790 113 58 6 6 967 967 967 967 94.2 89.1 93.1 89.9 97.5 92.2 88.3 82. 2 With a perfect stand, under the system of planting followed on this acre, there would be living plants in each of the 967 hills. Owing to the prevalence of missing hills, however, the stand has been more or less imperfect each year, as shown by the percentages given in Table I. Aside from the missing hills the crop is further influenced by the constant occurrence of unproductive plants. Of these, there are three classes : The male plants, of which a small number is con- sidered essential by American hop growers; the "bastard," or mon- grel, plants, the frequent occurrence of which is restricted to certain localities; and the normal female plants which from one of several causes are nonproductive. When yield is considered, the non- productive as well as the missing hills must be taken into account, since the yield per acre is directly proportional to the number of productive hills. The percentage of productive stand, by which is meant the percentage of bearing hills, is an important inaex of produc- tiveness, and on the acre in question tins figure shows, as may be seen from Table I, that each year about one-tenth of the hills are wholly unproductive. The records of the individual hills show some of the important reasons for the variation from year to year in the number of missing hills. The two chief causes of this variation are the more or less successful yearly replanting and the annual occurrence of new missing hills. The variation in these factors is numerically expressed in Table II. Table II. — Annual variation in the number of replanted and missing hills of hops on 1 acre. Factors of variation. 1909 1910 1911 1912 No record. ...do 12 57 21 r 44 22 9 15 3 ...do 110 56 66 24 113 ■ [Cir. 112] EELATION OF STAND TO YIELD IN HOPS. 29 The importance of replanting and the success with which it has been carried out on the acre under observation may readily be judged from Table II. In 1910 less attention was bestowed upon the replanting than in the two succeeding years, with very obvious results. Were it not for the continuous dying out of the hills an almost perfect stand could readily be attained. It is important to note that each year there was added to the list of missing hills a number that previously had been productive. In fact, it frequently occurs that a hill which has been producing heavily for several years suddenly becomes "missing." Of the 110 new missing lulls recorded in 1912 the average yield for the previous year was 10.2 pounds green weight, and 56 of these hills had each given a liigh yield in the years 1909 to 1911, inclusive. Out of the entire number of hills on this acre only 1 has been missing for the whole period of four years, 4 have been missing for three consecutive years, and 45 for two years in succession. Of the 56 lulls missing in 1909 only 6 were still missing in 1912. Altogether, 193 different lulls have been missing on this acre during the past four years, which would have necessitated the replanting of more than 20 per cent of the entire number of hills if a perfect stand were to be main tamed. The fact that new missing Mils occur each year, many of which have previously been highly productive for several 3-ears, strongly suggests that the average length of life of the culti- vated hop plant may be less than is popularly supposed. Cases are known where it is claimed that individual plants have given a fair yield each year for 30 years, but many growers agree that, with a newly planted yard, after three or four crops have been harvested the hills begin to die out to a greater or less extent. Positive con- clusions on this point, however, can not be drawn from the data in hand, since the period covered by the observations is entirely too short to be more than suggestive. VARIATION IN PRODUCTIVE STAND. A large part of the variation in productive stand is caused by the occurrence of lulls having vines producing no hops. Such hills pre- sent a greater problem than those which are missing, since many of them if left undisturbed produce a good crop the following year and digging them out and setting new plants might result in loss rather than gain. Each year a few of the replants fail to bear hops; others of the hills are probably unproductive through loss of vigor, since a number are dead the following year, and some vigorous and normally productive hills through some accident fail to yield a crop. How these various classes among the hills having vines but no hops are distributed from }^ear to year is shown in Table III. [Cir. 112] 30 CIKCULAK 112, BUREAU OF PLANT INDUSTRY. Table III.— Record of the hills on 1 acre having vines but jrroducing no hops, for the years 1909 to 1912, inclusive. Total hills. Re- planted hills. Productive hills. Nonproductive hills. Hills dead the Year. Previous year. Follow- ing year. Previous year. Follow- ing year. following year. 1909 43 21 = 50 5S 2 19 3 C) 17 25 51 36 18 37 0) 2 6 4 1 1 5 0) 6 1910 2 1911 S 1912 (') 1 No record. 2 The crop on 24 of these hills was lost through defective supports, which allowed the vines to fall to the ground. This table shows that the relation existing between the newly replanted hills and those having vines but no hops is less close than is generally supposed, since the number of the latter which were replants is small both in comparison with the total number of hills having vines producing no hops and with the number of hills successfully replanted, as shown in Table II. The figures in columns 4 and 5 of Table III indicate that of the hills having vines but no hops in any given year the greater number were productive in the previous year as well as in the one immediately following. Similarly, the figures in columns 6 and 7 show that very few of these hills were nonproductive in either the previous or the following year. Finally, from the last column it appears that relatively few of the hills having vines but producing no hops are numbered with the dead the following year. In view of the facts here presented there seems no escape from the conclusion that a large number of the cases of lulls having vines but no hops arise through neglect or carelessness in cultivating or caring for the plants up to harvest time. LOSS IN YIELD DUE TO DEFECTIVE STAND. Everyone recognizes that, as a rule, a poor stand means a diminished yield, but it frequently happens that the extent of tins loss is not fully appreciated. This is particularly true when the number of missing or nonproductive hills is small, for then the grower often feels that the saving would not be large enough to warrant his giving the time and attention necessary to maintain a full productive stand. This impression is likely to persist unless some relative numerical expression is found that will approximately represent the extent of the loss. A fairly satisfactory method of estimating loss is to deter- mine the percentage of productive stand and the actual yield, say on 1 acre, and from these figures to calculate what the yield would be on the basis of a productive stand of 100 per cent. The difference between the estimated yield and the actual yield will then represent [Cir. 112] RELATION OF STAND TO YIELD IN HOPS. 31 the logs. Applying this method to the records of the acre discussed in the previous paragraphs the results set forth in Table IV were obtained. Table IV. — Estimated loss and comparison of actual with estimated yield on 1 acre of hops. Year. Pro- ductive stand. Actual yield, dry weight. Estimated yield with full pro- ductive stand. Estimated loss due to lack of stand. 1 Quantity. Value. 1909 Per cent. 89.1 S9.9 92.2 82.2 Pounds. 1,487 1,443 2,353 1,828 Pounds. 1,668 1,605 2,552 2,223 Pounds. 181 If'..' 199 395 $29. 32 1910 15.87 191 1 i.-i. 27 1912 50. 56 i The estimate of value is on the basis of the farm value of hops in cents per pound, less 6 cents per pound for harvest costs. These farm values are officially estimated by the Bureau of Statistics, U. S. Dept. of Agriculture, as follows: 1909, 22.2 cents; 1910, 15.8 cents; 1911, 38.3 cents; 1912, 18.8 cents. When the effect on yield of missing and unproductive hills is thus translated into terms of dollars and cents per acre, the results of inattention to proper cultural methods become very clear. The aver- age loss on this acre for the four years 1909 to 1912 was $40, a sum certainly well in excess of that required to pay for the labor and supervision necessary to maintain a maximum percentage of pro- ductive stand. SUGGESTED PROCEDURE FOR MAINTAINING A GOOD STAND. Although some growers succeed in maintaining a practically per- fect stand, others may fail to do so owing to causes clearly beyond their control. However, strict attention to the suggestions which follow will eliminate nearly all of those cases of missing or nonpro- ductive hills which are due to carelessness or neglect. Such cases, as is shown on previous pages, are responsible for the greater part of the loss due to defective stand. PRACTICAL SUGGESTIONS. (1) Just before harvest time mark by means of stakes driven well into the ground all missing, "bastard," and excess male hills. After harvest dig out these hills and leave an open excavation at least 3 feet across and 2 feet deep. (2) At pruning time dig out all hills that have died during the winter; then, before replanting, fill the site of all excavated hills with fresh soil mixed with manure. (3) If possible, replant early while the soil contains an abundance of moisture to support the growth of the cuttings; cuttings planted in dry soil or sand should be well watered when they are set out. [Cir. 112] 32 CIRCULAR 112, BUREAU OF PLANT INDUSTRY. (4) In replanting use only sound, vigorous cuttings taken from high- yielding hills and see that the cuttings are not allowed to dry out before planting. (5) After the plants are well started inspect the hills carefully and replace all weak or dead plants with vigorous reserve plants from the nursery. (6) Personally supervise the work of replanting, especially when it is done under contract or when immigrant labor is employed. (7) In pruning, carefully distinguish (a) normal, well-developed stocks, which may be cut back either quite close to the crown or so as to leave only the first set of eyes on the stumps of the vines of the previous year, and (b) small, weak stocks, which should be so cut that two or even three sets of eyes will be left on the stumps of the vines. (8) See that the vines are properly tied up, so that they will not be caught and broken or torn down by the implements used in cultivat- ing or spraying. (9) Keep a constant oversight of the fields and whenever a vine is torn down or falls to the ground see that it is immediately replaced on its proper support. [Cir. 112] ADDITIONAL COPIES of this publication -Ti- may be procured from the Superintend- ent of Documents, Government Printing Office, Washington, D. C. , at 5 cents per copy U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 113. B. T. GALLOWAY, Chief of Bureau. MISCELLANEOUS PAPERS. Soil Bacteriology as a Factor in Crop Production A New Ornamental Palmetto in Southern Texas . Commercial Truck Crops on the Truckee-Carson Project K. F. KELLERMAN 0. F. COOK |F. B. HEADLEYand VINCENT FULKERSON A Purple-Leaved Mutation in Hemp The Tuber-Unit Method of Seed-Potato Improvement . L. H. DEWEY WILLIAM STUART Issued February 15, 1913. WASHINGTON: GOVERNMENT PRINTING OFFICE. 1913. [Cir. 113] 2 BUREAU OF PLANT INDUSTRY. rhief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A.Taylor. Editor, J . E. Rockwell. chief Clerk, James E. Jones. ADDITIONAL COPIES of this publication 11 may be procured from the Superintend- ent of Documents, Government Printing Office, Washington, P. C, at 5 cents per copy SOIL BACTERIOLOGY AS A FACTOR IN CROP PRODUCTION. 1 By Karl F. Kellerman, Physiologist in Charge of Soil-Bacteriology and /'laid Nutri- tion Investigations. IMPORTANCE OF SOIL BACTERIOLOGY. This day and generation are in the grasp of the invisible, the things infinitesimal, the things minute. The physician and the engineer have learned that they can battle with the complex conditions of to-day only by understanding a wealth of microscopic detail that formerly would have been considered ridiculous. These two pro- fessions have now joined with biology in recognizing the microscope as a necessity. It is not surprising, therefore, that the most impor- tant profession in the w r orld, that of farming, should awaken to the desire of comprehending and controlling the tremendous although imperceptible forces which may enrich or ruin fields and crops. It no longer thrills an audience to say that soil is a living tiling; it is either a trite remark, a commonplace relegated to the shelves as uninteresting, or it is a statement quoted impressively to explain everything in soil fertility. Both attitudes are unfair to this new science. Real progress is slow, and there are good reasons for asking the farmer to maintain a deep interest in the unseen population of the fields and to beware of dogmatic explanations unsupported by properly marshaled facts. The average mind is more than willing to believe a fairy tale, but unquestioning belief in hasty conclusions is more injurious to future progress than temporary lack of interest or appreciation. Most readers of modern agricultural literature are familiar with the generalization that but for the various agents of decay the world would in a very short time become uninhabitable. It is through the constant disintegration and decay of dead plants and animals that fertile soils are regenerated and that the processes of growth of plants and animals can continue. Oidy during the last half century has the science of bacteriology shown how remarkable is this transmutation of the dead plants and animals back to soil. Bacteria themselves are plants. They form the simplest group of the fungi, or plants that are lacking in chlorophyll. They are exceedingly minute; the majority 1 Issued Feb. 15, 1913. This paper was presented Jan. 7, 1913, as one of a series of lectures given before the scientific staff of the Bureau of riant Industry. [Cir.113] 3 4 CIECULAK NO. 113, BUREAU OF PLANT INDUSTRY. are not more than one one-hundred-thousandth of an inch in diameter, and it is believed that some bacteria exist which are too small to be seen even with the aid of the most powerful microscope. In spite of then small size, however, they are concerned with every phase of our daily life and by their incredible numbers and ceaseless activity over- come their apparent insignificance. Bacteria cause diseases, make milk sour, and in many ways are most troublesome. In spite of the evil that some species of this group of plants cause, however, other species, and even some of the troublesome species under different conditions, are beneficial. CLASSES OF SOIL BACTERIA. The bacteria of the soil are chiefly of the beneficial types. They occur in almost infinite numbers, a fertile soil having from 15, 000, 000 to 300,000,000 to the ounce. Their functions and value are variable, both because the kinds of bacteria differ in soils and because any given species may vary physiologically within certain limits according to environmental conditions. The moisture, the temperature, the degree of pulverization, the rock formation, or the geological history of the soil, the aeration, the drainage, etc., are all factors which partly determine the action of soil bacteria; and perhaps more important than any of these limiting conditions is the effect of one kind of organism upon those with which it is closely associated, or, more broadly speaking, the effect of the associative or competitive action of the various groups of micro-organisms which act and react upon each other. In outlining briefly the relation of soil bacteriology to crop pro- duction it is simpler to consider the subject from the standpoint of the bacteriologist and refer to groups of organisms more or less well known that perform different functions in maintaining the fertility of farm soils. These groups taken in their entirety include practically all of the known types of decomposition and synthesis which take place in the soil. They include organisms which produce hydrogen-sulphid gas, indol, mercaptan, and other substances of this character whose importance in agriculture has yet to be demonstrated and need not be considered at this time. The chief groups and the order in which they will be discussed are the carbohydrate fermenters, which change starch, sugar, cellulose, etc.; the ammonifiers, which decompose com- plex nitrogenous compounds and form ammonia; the nitrifiers, which oxidize ammonia to nitrite and nitrite to nitrate; the denitrifiers, which reduce nitrate to nitrite and sometimes to free nitrogen gas; and the nitrogen fixers, which "fix" or combine the free nitrogen gas of the air with other substances to form proteids. (Cir. 113] SOIL BACTERIOLOGY AS A FACTOR TX CROP PRODUCTION. 5 CARBOHYDRATE-FERMENTING BACTERIA. The group of organisms fermenting carbohydrates, such as sugar, starch, and especially cellulose, which is the substance forming the firm or fibrous portions of plants, has many representatives, but their functions and their relations to crop plants are in most cases not well understood. It is known, of course, that many species of bacteria will ferment the different sugars, forming some organic acid and fre- quently carbon dioxid and hydrogen or carbon dioxid and methane. It is highly probable that the constant production of these gases, in minute quantities it is true, in the chemically powerful condition of the nascent or freshly liberated gas exerts a much greater influence in soil weathering and the solution of plant food from small particles of minerals than any of the much-discussed agencies of the plant roots themselves. Both the carbon dioxid and the organic acids produced by these organisms are presumed to be of importance in controlling the availability of phosphates. No satisfactory methods of con- trolling this action have been developed, however, and no accurate estimate of this function has been possible. The most resistant carbohydrate found in the soil and the type of carbohydrate which is most important because of the enormous quantities added to soils annually is plant fiber or cellulose. This is a substance so resistant to even moderately strong acids and alkalis that it appears at first glance almost indestructible. Obviously, however, active agents for cellulose destruction must be constantly operating; the decay of wheat stubble, of cornstalks, and of green manure turned under, as well as the rapid rotting of dead trees, are perhaps the best illustrations of this. It should be noted that humus has long been recognized as an essential constituent of a fertile soil, and more recently it has become apparent that many of the processes constantly going on in the soil which result in the formation of avail- able plant food are controlled in a large measure by the quantity and quality of the humus compounds. In the formation of these com- pounds the cellulose, which is decomposed in the soil in such large quantities, must play a prominent role. Extensive studies in our laboratory during the past two years have shown that there are numerous organisms endowed with the power of actively fermenting cellulose. The cellulose is made soluble and becomes available as carbohydrate food for other organisms, especially the nitrogen fixers, which will be referred to later. There is another phase of cellulose destruction which appears to be of great economic importance from the soil-fertility standpoint, and that is the action of filamentous fungi. Several investigators from time to time have found one or more species of molds to which they [Cir. 113] 6 CIRCULAR NO. L13, BUREAU OF PLANT INDUSTRY. have attributed the power to dissolve cellulose, but no systematic attempt has been made to determine the extent of this phase of cellulose destruction. It appears, however, that cellulose destruc- tion by molds is as important, if not more important, than its destruc- tion by bacteria. There are at least 75 species of cellulose-destroy- ing molds, representing a large number of genera, including such common forms as Aspergillus, Fusarium, and Penicillium, which is the green mold so often seen on fruit and moist hay. The enor- mous quantity of cellulose available in the soil and the tremendous energy that can be developed by bacteria in breaking up the cellulose into simpler ferments make it seem at least possible that the mainte- nance of a high degree of fertility depends more upon the presence in the soil of the proper flora for decomposing the cellulose than upon the existence of any other group. It should be remembered that this accumulation of carbohydrate food, the source of energy for so many soil processes, is due to the fact that chlorophyll, the green coloring matter of the leaves of plants, in some manner as yet unexplained, enables the plants to absorb the carbon dioxid of the atmosphere and to utilize the energy of the sunlight to transform this carbon dioxid and the water of the plant sap into carbohydrates. The synthesis of carbohydrates is logically the most fundamental ques- tion of plant physiology, for this great quantity of potential energy is chiefly responsible for the continuance of plant and animal life. AMMONIA-FORMING BACTERIA. There are many species of bacteria very widely distributed in nature which break down proteid compounds, forming ammonia together with other decomposition products. These ammonifieis, indeed, comprise what is numerically perhaps the largest group of soil organisms. It was formerly supposed that crop plants were 1 unable to assimilate nitrogen in the form of ammonia. The pro- duction of ammonia by bacteria was therefore regarded as merely a preliminary step in the preparation of suitable plant food. More recent investigations show clearly that many crop plants, especially cereals, are able to assimilate ammonia nitrogen. The ammonifieis therefore do prepare a suitable food for crop plants, and because of the rapidity of their action and because of the enormous number of them that are found in all soils we shall probably have to con- sider this the most important group of bacteria. The fact that they are widely distributed, however, makes them somewhat less interesting from the standpoint of controlling fertility, since it is doubtful whether conditions obtain in any agricultural region where these bacteria are not present in sufficient numbers to transform rapidly into ammonia whatever proteid nitrogen may be turned [Cir. 113] soil, BACTERIOLOGY AS A FACTOR IN CROP PRODUCTION. 7 into the soil, provided conditions for bacterial growth are favorable. In this connection it is interesting to note that the Subtilis group, a subdivision of the group of ammonifiers comprising four or five distinct species, occurs probably in every cultivated country. It has been found in every country where any attempt has been made to isolate these species. The products formed by the various species of bacteria in the ammonifying group, in addition to the ammonia formed from the proteid nitrogen, are not well known. It is there- fore a possibility that some of these bacteria may produce substances injurious to the growth of crop plants. The so-called soil toxins, which may or may not be important factors in limiting the develop- ment or continued cultivation of certain crops, may be produced by these bacteria; there is as yet, however, no satisfactory evidence that such is the case. NITRIFYING BACTERIA. The nitrifying bacteria are in reality two groups of organisms. The first group when supplied with mineral plant food and with ammonia nitrogen transforms the ammonia into nitrite. This group, which formerly was considered to consist of a few very highly special- ized organisms, is now known to number many species, most of which, however, have the power of making the transformation of ammonia to nitrite only to a moderate degree. The large number of organisms having this power makes it seem probable that usually they may be more important than the few species that are more active and also are more seldom found. The second group of nitrifying bacteria is able to further oxidize the nitrogen in the form of nitrites to nitrate nitro- gen. Nitrate nitrogen is the form of nitrogen usually considered to be of the highest value as plant food, while nitrite nitrogen, except in very minute quantities, is an actual poison to crops. This special- ized group of bacteria, therefore, which is able to feed on nitrate nitrogen and as fast as it is produced transform it into desirable food, is of extreme importance in all cultivated soils. Like the ammoni- fying bacteria, however, the bacteria of this group also are widely distributed and usually are present in sufficient numbers to perform their proper functions. They develop best in soils that are kept in good tilth, and usually grow very sparingly, if at all, in water-logged acid soils, and but sparingly in the fine-grained clays and gumbos that are almost impervious to the air. DENITRIFYING BACTERIA. The denitrifying bacteria, many of which are also ammonifying bac- teria, are those which have the power almost diametrically opposed to that of the two groups just described. These organisms trans- [Cir. 113] 8 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY. form nitrate into nitrite, perhaps also into ammonia, and certainly many of them are capable of breaking up the molecule entirely and giving off free nitrogen gas. This group of organisms if functioning in this manner constantly would soon destroy the most fertile land, but although these organisms are found generally, they are not usu- ally very plentiful and they do not become active denitrifiers unless afforded certain definite conditions for growth. The requirements of different species of the denitrifying group vary considerably; most of them will transform nitrate nitrogen into nitrite if organic material, such as beef broth or plant extracts, or even manure, be supplied them together with nitrate nitrogen. This fact illustrates the danger of applying nitrate fertilizers and manure simultaneously to a crop. Especially if the soil is moist or water-logged at the time there ensues a rapid development of nitrites with consequent injury to the crop. If nitrates and manure are to be applied to a given field, the manure should be applied weeks or, preferably, months in advance, in order to allow the preliminary decomposition of the proteid compounds in the manure to proceed to a point where they will no longer be food for denitrifying bacteria. NITROGEN-FIXING BACTERIA. The nitrogen-fixing bacteria are those which have the power of combining the atmospheric nitrogen with their other food materials and forming proteid nitrogen. Their activity can be enhanced by the improvement of the soil tilth, and sometimes also by the addition of carbohydrates, such as sugar and molasses. It is probable, however, that cellulose which has been decomposed to some soluble form is as good a food as any of the sugars produced commercially. Since the development of these free-living, nitrogen-fixing bacteria is presumed to be largely responsible for the continued fertility of agricultural land and since it is probable that these bacteria are the chief cause of the undiminished fertility of the lands of the intermountain district in the Northwest, where continuous grain cropping has produced no apparent diminution hi soil nitrogen, both the importance of this group of bacteria and the probable correlation of the development of nitrogen- fixing bacteria with cellulose-dissolving bacteria are obvious. NODULE-FORMING BACTERIA. An important subdivision of nitrogen-fixing bacteria and one which is perhaps better known than any other group of soil bacteria is the nodule-forming bacteria of the legumes. These bacteria when associated with the roots of the leguminous plants and a few non- leguminous plants form nodules upon the roots which are capable of using as food large quantities of atmospheric nitrogen. The recipro- [Cir. 113] SOIL BACTERIOLOGY AS A FACTOR IN CROP PRODUCTION. 9 fating or complementary action of the different groups of soil bacteria which transform nitrogen compounds is more clearly expressed by a diagram. Figure 1, which is self-explanatory, shows the relation of bacteria to some of the most essential changes. Since the study of the nitrogen-accumulating power of legumes bearing root nodules BAC TERIA CHANGE NITROGEN GAS INTO PFtOTED NITROGEN BACTERIA OF LEGUME AND NON-LEGUME ROOT NODULES CHANGE NITROGEN GAS INTO PROTEID NITROGEN DENITRIFYING BACTERIA <-«« o o y # Fig. 1.— Diagram showing the nitrogen changes produced in the soil by the action Of bacteria. The arrows indicate the course of the changes which various groups of bacteria may produce in the n it r< men compounds of the soil. deals with a definite and comparatively simple relation of a single type of organism to a single type of plant, it has been possible to develop these investigations upon a pure-culture basis; in other words, it has been possible to isolate the nitrogen-fixing organism from nodules of leguminous plants and with these cultures produce 77106°— Cir. 113—13 2 10 CIRCULAK NO. 113, BUREAU OF PLANT INDUSTRY. nodules upon other plants of the same species in different localities. Owing to the fact that different legumes are constantly being intro- duced into agricultural regions, the importance of being able to dis- seminate the nodule-forming bacteria is obvious. For many types of legumes this is desirable, not only from the standpoint of making the crop a better nitrogen fixer and better soil renovator, but because for most legumes the crop is actually larger when properly inoculated. Pure-culture inoculation is less certain than inoculation by means of soil from old, well-inoculated fields, though of course it is free from the danger of introducing troublesome weed seeds or plant diseases. WORK OF THE BUREAU OF PLANT INDUSTRY. The Bureau of Plant Industry is carrying on field experiments to determine, if possible, what soil conditions are most favorable for the successful inoculation of leguminous crops by the use of pure cultures and also to determine under what conditions it is useless to attempt to inoculate certain of the legumes without some radical change in the method of fertilizing or cultivating these fields. To extend this experimental work as far as possible, the Department of Agriculture is willing to supply cultures in any reasonable quantity, requiring only the filling in of blank reports which are occasionally forwarded for this purpose. CONCLUSION. A casual review of the present status of soil bacteriology shows that it is a subject of almost bewildering complexity, but very intimately associated with the normal physiology of all crop plants. By learning what functions must be performed in the soil and by studying each group of organisms that has a measurable function, we believe that we can learn how to enhance the desirable activities of thaliving soil and to check the undesirable ones. We may never find another rela- tionship so simple as that existing between the legume crop and the nodule bacillus or one which we can control so simply from a central laboratory; but surely a thorough comprehension of what happens in the soil will teach us how to maintain the fertility of the soil more surely than blind and rather purposeless experimentation. The great majority of plat tests of fertilizers illustrate the impossibility of satis- factory deductions from experiments entirely upon an empirical basis. We must deal in irons and not in generations if agricultural science is to be advanced by the cut-and-try method. [Cir. 113] A NEW ORNAMENTAL PALMETTO IN SOUTHERN TEXAS. 1 By O. K. i'ook, Bionomist in Charge of Crop Acclimatization and Adaptation Investigations. Apart from the date palms, which are of Old World origin, only two kinds of palms have qualified for general planting in southern Texas. These are the Washingtonia palm, a native of the desert region of south- ern California, and the palmetto. Several times in recent years the Washingtonia palms in the city parks of San Antonio have had tneir leaves partially killed by cold weather, while adjacent palmettos re- mained entirely uninjured. Thus the palmettos may be reckoned as the hardiest of all of the native North American palms. The minimum temperature recorded at San Antonio in the winter of 1910-11 was 16° F. and in the following year 18° F. At Victoria, Tex., the cultivated palmettos have passed without any damage to the leaves through freezes that killed many of the wild huisaches (Acacia farnesiana) . Though certain other palms are able to survive such temperatures and are worthy of being planted for special pur- poses, the mutilation of the leaves means a loss of decorative value for several months. Frost-proof foliage is especially desirable in an ornamental species. There is a native Texas palmetto (Inodes texana), which is not known to exist in a wild state except in the vicinity of Brownsville;- but it seems to have extended much farther northward only a few decades ago, and specimens may still be found about Indianola or at other points along the Gulf coast. No doubt the same species extends into adjacent regions of Mexico, but it differs from the palmettos of other parts of that country. Sargent and other writers have referred the Texan palmetto to Sabal mexicana Martius, but this species was based on a small t runkless palm from a remote locality on the southern coast of the State of Oaxaca, not far from the Isthmus of Tehuantepec 1 Issued Feb. 1".. L913. 2 This is in addition to the low, creeping, scrub palmettos which are widely distributed in swamps and wet river bottoms of the Coastal Plain. The scrub palmettos belong to the genus Sabal, which was formerly supposed to include both groups of palmettos. The genus Inodes differs from Sabal in the formation of a thick, upright trunk by secondary thickening of the fibrovascular system below the terminal bud. As a consequence of this habit of growth the leaf bases are split down the middle, as in i he Washingtonia palms, but not in Sabal, Erythea, or Brahea. Another difference is that leaves of Sabal are few in number and deeply divided in the middle, because of the slight development of the midrib. The strongly specialized recurved midrib is a peculiarity of the genus Inodes. [Cir. 113] 11 12 CIECULAE NO. 113, BUREAU OF PLANT INDUSTRY. The palmettos planted for ornament in San Antonio and other plaees in southern Texas were at first supposed to represent the native Texan palmetto. They have broad leaf segments like Inodes texana, instead of narrow segments like the well-known cabbage pal- metto of Florida {Inodes palmetto), of which only a few specimens have been seen in Texas. The Texan palmetto is a handsome palm and is used for ornamental purposes in a few places in the region of Brownsville, but it now appears that other species are represented among the cultivated palmettos. This was learned recently at Victoria, Tex., where one of the cultivated palms was found with an abundant crop of fruit. The thickened terminal branchlets of the inflorescence showed at once that it could not be the Texan palmetto, which has slender branchlets. Some of the Victoria palmettos are really magnificent, with their stately crowns of large vivid-green leaves firmly supported on massive petioles, also of living green. Even the trunk appears green, for the sheathing leaf bases retain their color. The crown is more ample than in most palms because of the firm texture and persistent vitality of the leaves. This lends an impression of extreme vigor and luxu- riance and adds greatly to the decorative effect. In short, it seems not unlikely that the Victoria palmetto may find a place in the front rank of ornamental species. Inquiries regarding the origin of the palmettos at Victoria revealed a local opinion that they had been brought from California, and this may indicate that the stock came originally from Mexico. There are no native palmettos in California. Many introduced species are grown in collections, though they do not seem to thrive very well in places near the coast, probably because of insufficient heat. At first it seemed likely that the Victoria palmetto might be referred to a species with thickened branchlets, Inodes uresana Trelease, from the vicinity of the town of Ures in Sonora. 1 But there are several features that seem to forbid such an identification. The leaves of Inodes uresana are described as extremely glaucous, to such an extent that Prof. Trelease compared it with the so-called "blue palm" (Erythea armata), noted for its very light grayish or bluish color. This characteristic does not appear in the Victoria palm, the foliage of which is of a deep, vivid green, as far from glaucous as could well be imagined. To judge from the photographic illustration of Inodes uresana, the leaves have a more strongly decurved rachis and more deeply sepa- rated, narrower segments. The measurements given for the Sonora n species likewise indicate a smaller palm. The trunk is said to be 30 centimeters thick, the petiole 2 centimeters wide, and the blade 1 i Trelease, W. A Pacific-slope palmetto. Missouri Botanical Garden Keport, v. 2, p. 79, pi. 35-37, 1901. [Cfr. 113] A NEW OENAMENTAL PALMETTO IN SOUTHEKN TEXAS. L3 meter wide, or about only half the size of the corresponding parts in the palms at Victoria, where the individual leaf segments arc nearly a meter long and 5 centimeters wide. The Victoria palmetto agrees with Inodes uresana in the size of the fruit and seed, but the seed is not coarsely wrinkled above as in Inodes uresana nor hollowed below. The absence of any distinct exca- vation of the ventral surface renders the seeds of the Victoria pal- metto quite distinct from those of any of the large-seeded palmettos in the National Herbarium or in the seed collection of the United States Department of Agriculture. Instead of being hollowed out, the lower side of the seed is flat or slightly convex and usually shows a small, smooth prominence in the middle, surrounded by coarse, irreg- ular wrinkles. The hilum is eccentric, but close to the central promi- nence and surrounded by stronger radiating wrinkles. The remainder of the surface, outside the flattened areas of the lower side, is not wrinkled but only finely coriaceous or leathery under a lens. The micropyle, marking the position of the embryo, is represented by a very distinct conical elevation surrounded by a flat rim or a slight circular depression. The embryo is lateral, just above the transverse middle of the seed. The seeds are about 7 millimeters thick, with a diameter of from 10 to 12 millimeters, about two-thirds of them being 11 millimeters and less than 10 per cent attaining 12 millimeters. One of the seeds showed no micropyle, embryo, or cavity in the endosperm. The seeds are surrounded in the mature fruit by a thin membrane of rather loose corky texture, with the outer surface torn into numerous spinelike points by the shrinkage of the pulp which dries down against the outer wall into a brownish layer, usually less than 1 millimeter thick. This leaves an empty space, often 2 millimeters across, between the membranous coating of the seed and the similar coating of the inner surface of the pulp. The total diameter of well- developed fruits is about 18 millimeters and the length nearly the same, owing to the presence of a somewhat oblique narrowed base bearing the conical persistent style as a spinelike basal projection nearly 2 millimeters hi length. Only one fruit is developed from a flower. There are none of the twin and triplet fruits that are of frequent occurrence in Inodes texana. Another related species from the Pacific slope of Mexico is Inodes rosei Cook, described from specimens collected by Dr. J. N. Rose, at Acaponeta, Tepic, but this palm has the ultimate branchlets veiy slender and the seeds like those of Inodes uresana, strongly rugose above, and deeply umbilicate below. The fruits of Inodes rosei are usually smaller than those of the Victoria palmetto, with thinner membranes and less pulp. The lobes of the calyx are shorter and the persistent [Cir. 113] 14 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY. style small, shriveled, and appressed instead of forming a prominent spur. On account of the divergencies from the related forms it seems necessary to recognize the Victoria palmetto as a new species, for which the name Inodes exul is proposed, in allusion to the fact that the original habitat is unknown, though there is every probability that it will be found in some part of northern Mexico. The diagnostic characters at present available are the large size, the deep-green foli- age, the thickened branchlets of the inflorescence, the solitary fruits, and the large seed not wrinkled above nor hollowed out below. Type specimens and photographs have been deposited in the United States National Herbarium (No. 691415) and a sample of the seeds in the Economic Herbarium of the United States Department of Agriculture. Other characters of the species remain to be learned by further study, but it seems desirable to publish the present state- ment as a means of bringing so promising an ornamental to public attention. The type individual of the new species graces the lawn of Mrs. Martin O'Connor, of Victoria, Tex., who has kindly presented the seed to the United States Department of Agriculture through Mr. John H. Kinsler, of this Department. The seed lias been planted at the United States Experiment Farm at San Antonio with a view to wider distribution in southern Texas, where congenial conditions seem to be assured; and in view of the demonstrated hardiness of the species it may be expected to thrive in other parts of the coun- try, probably throughout the Gulf coast "and South Atlantic regions, as well as in the warmer parts of the Southwest. [Cir. 113] COMMERCIAL TRUCK CROPS ON THE TRUCKEE-CARSON PROJECT. 1 By F. F>. Headley, Superintendent, and Vincent Fulkerson, Scientific Assistant, Office of Western Irrigation Agriculture. INTRODUCTION. The growing of truck crops on the Truckee-Carson project is assum- ing increasing importance from year to year. This class of farming is largely followed by men who rent land on the older alfalfa ranches. Suitable land for this purpose has been rented for $12 to $25 per acre and generally for a term of years, since subduing and leveling old alfalfa land is often expensive. Potatoes, onions, celery, and cantaloupes are grown extensively for marketing outside the State, but the growing of other truck crops is limited to the demands of the local non-agricultural population resid- ing on the project and in adjacent mining camps. The future devel- opment of the truck-crop industry is therefore limited, except for those crops that will bear shipment and can compete with the outside market. POTATOES. About 480 acres of potatoes were grown on the Truckee-Carson Project in 1912. The Burbank is about the only variety grown for shipment. The yield per acre is exceedingly variable, owing to a number of causes, such as variation in soil, irrigation, and seed. The average yield on good alfalfa land is probably about 10 tons of market- able potatoes per acre, while the average yield for the entire project in 1912 was 4.1 tons per acre. Market prices in the last few years have ranged between $12 and $25 per ton. The future of the potato industry is promising. The local demand is large 1 , and the completion of the Panama Canal may so reduce the transportation rates that Nevada-grow T n potatoes can be placed in eastern markets. The quality of Nevada potatoes is excellent and by careful selection of seed and type, with due care in growing, sort- ing, and marketing, there should be a large development in this industry. i Issuerl Feb. 15, 1913. [Cir. 113] 15 16 CIRCULAR NO. 113, BUREAU OP PLANT INDUSTRY. Cost of 'production . — The following statement gives an estimate of the cost per acre of potato production in the Truckee-Carson Project: Rental cost of land $15 Plowing land 3 Leveling and harrowing 1 Seed 15 Planting 3 Cultivating 3 Irrigating, nine times 3 Digging 20 Sacks 15 Marketing 6 Total cost per acre 84 Numerous publications have treated of the growing of potatoes under irrigation, and the general principles of successful practice are as applicable to this region as to other Western States, yet ignorance of local conditions invites failure. Some of the special precautions necessary to observe are given below. Selection of seed. — No seed containing eelworms, produced either locally or imported, should be planted. Misshapen tubers and even good specimens from poor hills should not be used for seed. Selec- tion of good hills should be made in the field. A man can usually follow a digger and select such hills as yield abundantly and produce fair-sized, well-shaped tubers. Imported Oregon seed stock has been largely used and is considered most desirable by the more successful growers, but careful hill selection of suitable varieties promises better results in increasing the yield, as well as the percentage of smooth, marketable potatoes. Selection of land. — New land seldom gives good crops of potatoes, although some of the black tule lands are fairly productive. Gener- ally, only alfalfa land should be planted to potatoes, and the growers should not be satisfied with less than 9 tons (300 bushels) per acre. Either sandy or heavy loams are suitable if properly handled. A little white alkali (sodium sulphate) when present is not noticeably injurious, but the slightest traces of black alkali will injure the growth of the crop. Preparing land. — When alfalfa land is to be used for potatoes, the alfalfa should be ''crowned" in late summer or early autumn pre- vious to the year that potatoes are to be grown. By "crowning" is meant cutting off the alfalfa plants about 2 to 3 inches below the surface of the ground. To do this well a sharp plowshare is essential, and the plow must be strong, so that it can not be twisted or broken. After plowing, the field should be harrowed, so as to bring the crowns to the surface. If they are buried deeply in the soil they will [Cir. 113] { COMMEBCIAL TEUCK CROPS ON TRICK KIX 'ARSON PROJECT. 17 take now root and give trouble in cultivating the potatoes. In the spring the land should be again plowed, (> to 8 inches deep, and smoothed and graded to a uniform slope. A uniform grade is of great importance, as the water should at no time rise high enough to cover the tubers. A grade of 0.1 foot per 100 feet will irrigate, but a little more slope is rather better. It is usually advantageous to irrigate before the potatoes are planted, particularly if cut seed is used, as the dry soil absorbs the moisture from the cut potato. Varieties. — The early varieties are not recommended for shipping. Long-keeping and high-yielding sorts are better if the erop is to be shipped. The Early Rose is grown to some extent, but is generally not satis- factory, having too much waste from deep eyes. The Burbank, or some similar white potato, is the favorite, being more sought after in the California markets than other sorts. It is by no means certain that the Burbank is the most desirable variety from the growers' standpoint, as the tubers too frequently become knotty, rough, and pointed. A variety known variously as Netted Burbank, Netted Gem, and White Beauty has been tried in a small way. In shape it is better than the Burbank and it is productive. The Rural New Yorker and Colorado Pearl have done well where tried and seem to have less waste and fewer unmarketable tubers than the Burbank variety. It is probable that some such varieties will be found more profitable than the Burbank if no marketing difficulties are encountered. Planting. — The rows should not be more than 3 feet apart, for when farther than this there is the disadvantage of the waste of the land and the greater length of time required for the water to seep through under the hills. Most potato growers drop the seed 12 to 15 inches apart in the rows and cover to a depth of about 4 inches. Planting with a potato planter is more economical and satisfactory than furrowing with a plow and dropping by hand. A, man and boy with a team and planter will plant an acre quicker and easier than three men with a team and plow. Irrigating. — Potato soil should be kept as uniformly moist as possible. If the field is well irrigated before planting and thoroughly harrowed after planting it will not usually be necessary to water again until the sprouts are out of the ground. After the potatoes are up they will need irrigating in most soils about every 10 days until the crop begins to mature, when water should be held off entirely. There can be no set rules as to the frequency of irrigation, since some lands of heavy loam or with a high water table will need less water than others. The fact that the evaporation from an open [Cir. 113] 18 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY. water surface during the summer months is about 10 inches per month explains the necessity for irrigating oftener than in the less arid regions. Provision should be made to drain away the water that runs out at the ends of the furrows, so that it can not back up over the hills. The water must be kept in the furrows below the tubers. Irrigating in alternate furrows is not practiced here and probably is not advisable. Great care must be exercised in keeping the growth of the potatoes continuous and unchecked or the result will be knotty, badly shaped tubers. Cultivating. — It is a distinct advantage to cultivate after each irrigation until the vines interfere. The tool most commonly used is the winged-shovel plow. This keeps the furrow open and gives the land only partial cultivation. A spike-tooth cultivator with a large rear shovel to open the furrows gives better results. Diseases. — The most serious disease affecting the potato is the potato scab (Oospora scabies). No seed affected with this disease should be planted without thorough disinfection,' but it is preferable to use seed that has never been infected. Potatoes may be disin- fected by immersing the sacks containing them in a formalin solu- t ion for two hours. The solution is made by mixing 1 pint of formalin with 30 gallons of water. Another disease that results in considerable damage is that caused by the potato eelworm. Seed potatoes containing the eelworm should never be planted, as they not only injure the crop but per- manently infest the soil. 1 ONIONS. A large acreage of onions was planted on the Truckee-Carson Project in 1912, the plantings varying in size from 1 to 7 acres. One of the best fields was surveyed and found to have yielded slightly over 20 tons per acre. Cost of production. — An estimate of the cost of growing onions on this same field was also made and is given herewith, figured on a 1-acre basis. - ( lost of seed $8. 00 3£ days, hauling manure 7. 90 :U days, planting and irrigating 7. 90 J day, repairing ditches 1. 70 7 days, first cultivating and weeding 15. 75 <; days, second cultivating and weeding 13. 50 30 days, pulling, topping, and hauling 67. 50 Total cost 122. 25 i For more complete information in regard to potato culture, write for the following bulletins and circulars, which may be secured by sending requests to the Secretary of Agriculture, Washington, D. C: Farmers' Bulletins 407, The potato as a truck crop, and 3SG, Potato culture on irrigated farms of the West; Bureau of Plant Industry Circulars 90, Suggestions to potato growers on irrigated lands, and 91, The nematode gallworm on potatoes and other crop plants in Nevada. - All man labor is charged at the rate of .$2.'2. r > per day. [Cir. 113] COMMERCIAL TRUCK CROPS ON TRUCKEE-CARSON PROJECT. 19 If this farmer secures the normal market price of $20 per ton, making a total of $-100 per acre, he should net a handsome profit on this crop. Another grower having 7 acres of onions kept a careful account of expenses, which is given herewith, figured on a 1-acre basis: 1 Grading and leveling land $24. 70 Hauling manure 10. 30 5} pounds of seed 7. 20 225 pounds of commercial fertilizer 5. 30 Planting, tending, irrigating, and harvesting 104. 75 Total cost 152. 25 It is probable that with increased experience the total cost of pro- duction can be reduced below the sums mentioned. The cost of $24.70 per acre for preparing the land is more than will usually be found necessary. The yield of this field was not ascertained, so the profits per acre can not be calculated. The future of onion growing.— The price received during the last few years has ranged between $20 and $30 per ton. The character of the Nevada climate makes it comparatively easy to keep well- matured bulbs in inexpensive storage houses until the market becomes favorable, which usually occurs after January 1, when the bulk of the California crop has been marketed. Selecting and preparing hind. — None but the best soil should be planted to onions, as much land which produces ordinary farm crops at a profit fails utterly to grow marketable onions. Newly plowed alfalfa land may do well without manure, but generally a heavy application of manure should be plowed under in the fall or winter and a top dressing of fine, rotted manure applied in the spring before planting. After plowing, the soil must be thoroughly pulverized, smoothed, and graded, so that irrigation will be uniform. Varieties. — Only early-maturing sorts should be planted. As the Yellow Globe Danvers is productive, sells well, and is a good keeper, it is' to be recommended. If a white onion of reliable keeping quality can be found or developed, it should command a good market. The globe type is to be preferred, as the yield will be larger where all conditions are favorable. Planting and irrigating. — Planting should be done before April 1. With rows 12 to 1 .") inches apart, from 4 to 5 pounds of good seed will be required per acre. Onion seedlings are delicate and can not push up through a great depth of soil. The seed is usually planted at an average depth of three-fourths of an inch. The soil will require irrigation before planting, if it is not already moist enough to bring up the seed. The furrows should be made before irrigating or plant- ing. A suitable furrower can be made by using (> by fi inch timbers 1 All man labor is charged at the rate of $3 per day. [Cir. 113] 20 CIRCULAE NO. 113, BUREAU OF PLANT INDUSTRY. as runners. These should be set about 27 inches apart. Two rows can then be planted with a garden drill between the furrows. Irri- gation by flooding is also a common practice on this project, as well as in other onion-growing sections. If this method of irrigation is decided upon, the rows may all be the same distance apart. If the weather remains dry after planting, irrigation may be nec- essary to germinate the seed. A tendency of the soil to bake over the seeds indicates that irrigation is needed to soften the crust When furrow irrigation is used on soils that do not subirrigate well, much slope to the land is to be avoided; otherwise the water may run past without thoroughly wetting the soil about the plants. A slope of 0.2 foot per 100 feet should give satisfactory results, and if the soil is well supplied with humus more slope may be allowed, as such soil takes water easily and washes less. The irrigation furrows shoidd not exceed 300 feet in length. Often a field can be so arranged that the water which runs from the ends of the furrows may be utilized on a lower series. It is well to follow each irrigation as soon as practi- cable with a wheel hoe, so that the growth of the onions will not be checked by loss of soil moisture. Usually it is advisable to irrigate every week or 10 days until August. The market requires thoroughly matured onions which will keep well. To insure such, water should be withheld after the early part of August and the soil allowed to dry out. The bulbs should mature by September 15. A field full of scallions and immature bulbs indicates either poor soil, poor seed, late planting, or too much watering. Harvesting. — If the onions are properly matured, pulling is easy, and two or three days' curing in the field will put them in good condi- tion for the storehouse. It is entirely possible that it will be found the best practice where onions are properly matured to top them and put them at once into crates and store them in open sheds, where the curing will take place without injury from night frosts or the hot midday sun. Storing. — The important points in storage are dryness and low temperature. While a thin-board building, not frost proof, is better than a place too warm, a cheap adobe structure is especially adapted to this purpose, as any temperature in the fall or winter may be secured by regulation of the night ventilation, and the nonconductive adobe walls will prevent freezing. The bulbs can then be marketed at anv time. 1 CELERY. Celery growing is not yet an important industry. This crop has been very successful, and the produce is of excellent texture and ! For further information in regard to onion culture, see Farmers' Bulletins 354, Onion culture, and 434, The home production of onion seed and sets. |( ir. 113] COMMERCIAL TRUCK CROPS ON TRUCKEE-CARSON PROJECT. 21 flavor. There scons little prospect of overproduction, as much labor and care arc required to secure a good product. The crop is so valu- able that it has been shipped to distant towns in Nevada, as well as into California, where it sells at a higher price than the local product. Giant Pascal and Golden Self-Blanching arc good varieties. Only very rich soil should be used for this crop. The seed should be sown in April. A light mulch of old hay will prevent the baking of the soil and protect the small plants from the hot sun when they first come through the ground. The plants should be strong and stocky and about b inches high by the first week in July, when they are set in the field. The growth must be steady and rapid to secure good quality, upon which any extension of the market depends. Blanch- ing will require from 2 to 4 weeks. That part of the crop to be marketed in winter must be placed in pits to prevent freezing. 1 MELONS. Both watermelons and cantaloupes succeed well on the Truckee- Carson Project with comparatively little care. For home use any type of soil suited to ordinary crops will produce melons if well manured. For best results a retentive soil is required, one that is well subdued and well supplied with plant food. Much fresh manure and too much water tend to produce an inferior quality, though water needs to be applied regularly to keep the plants in thrifty condition and to insure steady growth. One gardener is growing both cantaloupes and watermelons for shipment, supplying the Southern Pacific dining cars and the whole- sale market in Reno. The varieties of watermelons grown are Kleekley Sweets, Halbert Honey, and Florida Favorite, and of can- taloupes, Emerald Gem, New Fordhook, and Rocky Ford. The Emerald Gem is the best quality, but skill is required in selecting properly ripened fruit and they do not stand shipment very well. The New Fordhook is very early and a much better shipper. The Rocky Ford is good, but later than the New Fordhook. Planting. — On rich, old alfalfa land rows 8 feet apart are none too far, as the vines will cover the entire surface. The hills can be 4 feet apart in the rows for cantaloupes and 6 for watermelons. Plant- ing can best be done before May 1, using old hay or straw to cover the young plants if severe frost threatens. After the litter has been distributed in a convenient place a man with a fork can cover a large number of plants in a short time on cold nights, and the advantage of an early start for the plants will well repay the trouble. Cultivating and irrigating. — There are no special requirements as to cultivation and irrigation, and the cost of bringing the crop to matur- 1 For further information in regard to celery culture, sec Farmers' Bulletin 2N2, Celery. [Cir. 113] 22 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY. ity is not large. Water should be applied often enough to keep a steady growth, which can be hastened by frequent cultivation. Too much water in the ripening period tends to retard ripening and reduces the sweetness of the melons. Harvesting and marketing. — Development of melon growing depends as much on proper care in picking and packing as upon growing. Only the best product carefully selected and neatly packed will justify shipment. The California product conies on much earlier and only an extra quality will insure sale late in the season when the Nevada crop matures. Watermelons should be ripening freely by August 15, for the late crop sells at low prices or can not be sold at all. Musk- melons can be sold successfully until October if the weather is such that the crop holds up in quality. They should be handled carefully and wrapped in paper in the packing shed, being then packed in crates 1 foot by 1 foot by 2 feet, outside measure. A crate of this size holds about 60 pounds. Watermelons for short shipment are packed in larger crates, but if the market will justify they are shipped loose in car lots. [Cir. 113] A PURPLE-LEAVED MUTATION IN HEMP. 1 By Lyster H. Dewey, Botanist In ( 'ho rye of Fiber-Plant Investigations. INTRODUCTION. Practically all of the hemp {Cannabis sativa) cultivated for fiber production in Kentucky and elsewhere in the United States is from seed of Chinese origin. The seed is nearly all grown in a limited area on the bottom lands along the Kentucky River. Hemp is dioecious, insuring cross-pollination, and since no attempt has been made to select or breed pure strains there has been a general mixing of all the strains introduced, resulting in a fairly uniform homo- genous type. The foliage of this hemp, so far as observed, is always a rich, (lark-green color hi healthy, normal plants. In 1912 two pistillate plants were found with foliage of a bright- purple color, in marked contrast to the normal green color. These grew in a small plat of hemp cultivated for seed selection on the Potomac Flats, Washington, D. C. The history of the seed from which this mutation sprang is definitely known for nine generations. SELECTION FOR IMPROVED HEMP. In the spring of 1903 about one-half of a pound of seed of the crop of 1002 grown in Kentucky was planted at the experiment sta- tion at St. Anthony Park, Minn. The seed plat was carefully cul- tivated, and oidy the heaviest seed from the one best pistillate plant was saved for planting. Cultivation and selection were con- tinued seven generations, including the crop of 1000, at the Min- nesota Agricultural Experiment Station. The plats were small each year and no other hemp was grown near enough to permit cross- pollination. It was therefore more closely inbred than often hap- pens in dioecious crops. Seed from the crop of 1000 was planted at the experiment station at Lexington, Ky.. in 1010, and again in 1911. A small plat, sown broadcast for fiber production at the Kentucky station, was more uniform than other hemp and produced fiber of better quality, but no marked difference in the foliage was observed in any of the plats, either in Minnesota or Kentucky. i Issued Feb. IS, L913. [Or. H3] 23 24 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY. FIRST APPEARANCE OF PURPLE FOLIAGE. Seed of the crop of 1911 in Kentucky was planted at Washing- ton, D. C, in 1912 and 60 well-developed pistillate plants and 46 staminate plants were produced. Two of the pistillate plants had purple foliage, strikingly different from the normal dark-green color of all others. One of these was the third best plant in the plat, and the other was above the average. The seed of the best purple plant was saved by itself. It is of a brownish color, easily distinguished from the normal gray of the other seeds, and it is larger in size than the others. Sometimes the wild hemp from bird seed has foliage with a copper- colored hue, and in 1901 a small plat of hemp from imported Hun- garian seed had several plants with copper-colored foliage, but none of these had the deep, rich purple color of the two marked plants in this seed plat of 1912. This is a singular mutation, showing strongly marked color variation hi foliage and seed, arising from a closely inbred strain of a uniform group of plants. ICir. 113] THE "TUBER-UNIT" METHOD OF SEED-POTATO IMPROVEMENT. 1 By William Stuart, Horticulturist, Horticultural Investigations and Arlington Farm. INTRODUCTION. The ever-increasing importance of the potato crop makes it highly desirable that more attention should be given to the subject of seed improvement. As yet comparatively little thought has been given to this phase of the potato industry by the American grower. The European grower, on the other hand, recognizes the futility of at- tempting to grow profitable crops of potatoes without giving the most careful attention to the source of his seed supply. To this end he either buys his seed from specialists in seed-potato production or becomes a specialist himself. The result of this attention to the use of good seed is well attested by the average yields secured. For the years 1901 to 1910, inclusive, the average per-acre yield in bushels was as follows: Germany 200.8, Great Britain 200.7, and the United States 92.7. It would be absurd, of course, to claim that all of this increase was attributable to good seed, but it is safe to assume that good seed does play a very important role and should not be over- looked by the American grower. DESCRIPTION OF THE "TUBER-UNIT" METHOD. The "tuber-unit" method was first recommended by Webber. 2 It consists for the first year in the selection from the seed bin before planting time of as many of the most perfectly shaped tubers as may be desired by the grower. These tubers should be selected with regard to type, size, and uniformity. Tubers ranging from 6 to 8 ounces in weight are preferred. Before planting, the selected tubers should be treated by immersing them two hours in a formalin solu- tion consisting of 1 pint of formalin to 30 gallons of water. In plant- ing, each tuber is quartered and dropped as cut. This is done by splitting the bud-eye cluster in four parts from seed to stem end of the tuber. In other words, the tuber should be cut through its longitudinal axis. The four pieces of each tuber are dropped con- 1 Issued Feb. 15, 1913. 2 Webber, If. J. Plant-breeding for farmers. New York Cornell Agricultural Experiment Station, Bulletin 251, Feb., 1908, pp. 322-331. [Cir. 113] 25 26 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY. secutively in the row at a distance of from 10 to 14 inches apart, according to the character and moisture content of the soil. By allowing an additional spacing between each set of fours the four plants from each tuber are definitely isolated from adjoining ones and the grower can readily observe any variation in vigor and uni- formity between the various units planted. It also enables him to detect any mixtures which may be present in the variety. If a potato planter with a fertilizer-distributing attachment is available, it may be used to open the furrows and drop the fertilizer by simply removing the covering disks and setting the plow a little deeper. The seed pieces may be covered with a plow if care is exercised not to displace them. During the growing season such observations as are indicated in the accompanying form of Note Blank I should be recorded (Table I). All units which do not satisfy the require- ments of a good strain, such as uniformity of type in habit of growth and character of foliage and size of plant, should be marked as undesirable units. This constitutes the first step in both the selection and elimination processes. At digging time the product of each unit is separately harvested by hand and a further selection made from the marked or surviving units. Only such units should be retained for further trial as most nearly approach the desired ideal, which in this case is uniformity in size, shape, and productive- ness. The tubers from each selected unit are collectively placed in separate sacks, preferably in cotton or burlap. Heavy manila paper bags may be used, but there is always danger of their being torn. Before being placed in storage, each sack should be numbered with the varietal and tuber-unit number, in order to preserve its identity in the field notes. The final examination should consist of notes, recorded on the note blank, of the number and weight of merchant- able and unmerchantable tubers. From each of the units not re- jected at this time select 10 of the best tubers for the next season's planting. It is desirable, though not absolutely essential, to main- tain the study of each selection on the tuber-unit basis, because it permits a more accurate comparison of the behavior of each. The 10 selected tubers from each original unit will give 40 plants for study the second year. As in the previous season, notes should be taken on the growing plants, recording them on Note Blank II (Table I) . All selections which do not produce a reasonably uni- form lot of plants should be marked for rejection. Each selection should be harvested separately, as in the previous year, and such as do not meet the requirements imposed should be rejected. The fur- ther conduct of the work will consist in the multiplication of the selected strains for field planting and the elimination of any weak plants that may occur during this process. [Cir. 113] " TUBER-UNIT " METHOD OF SEED-POTATO IMPROVEMENT. 27 VALUE OF THE "TUBER-UNIT" METHOD. The tuber-unit method was principally intended to serve as a ready means by which high-yielding or uniformly shaped strains could be isolated from the general mass. The writer conceives that its most valuable feature lies not in the isolation of highly produc- tive strains, but in the elimination of the unproductive and diseased plants. Extensive experiments conducted by this office show con- clusively that the simple elimination of the unfit tends to materially increase the per-acre yields. A careful study of strong and weak FIG. 1.— Strong (1) and weak (2) tuber units of Rural Blush potatoes. (Photograph of plants made about August 20, 1911.) tuber units of 12 varieties during the past two seasons, 1911 and 1912, shows in every instance a very close relationship between the vigor of the plant and the tuber yield. The average production of merchantable tubers from the strong plants was over 16 times greater than from the weak ones, while in the case of the culls the yield was only a little over twice as great. The data further show that the average weight of merchantable tubers from the strong plants was 5.3 ounces, as against 3.8 ounces from the weak ones. The small tubers or culls from the strong plants averaged 1.7 ounces, [Cir. 113] 28 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY, while the weak plants averaged but 1.1 ounces. The significance of Jp8 po W)8 S° (\|0 loo NO 50 000 ^o ■a o these data is fully realized only when considered in its relation to the use of large or small tubers for seed purposes. It is readily seen that but a very small proportion, if indeed any, would be included in the use of tubers weighing 5 ounces or over, whereas in the use of small tubers the proportion of small tubers from the weak plants would be, relatively speaking, much greater. Herein lies the danger in the use of small potatoes from unselected seed stock. The accompanying reproductions of photo- graphs shown in the top row of figure 1 repre- sent strong and weak plants of two tuber units of the Rural Blush potato in 1911 . These plants were produced from apparently identical tubers, were grown in the same row, and were subjected in every particular to identical cultural condi- tions. The reproductions in the middle row represent the tubers produced by these plants, while the lower set represent tne tubers pro- duced in 1912 from five tuber units of the 1911 crop. The 1912 crop is divided into two classes, according to size. The tubers on the left in each illustration consist of those weighing 3 ounces or over. These are designated as mer- chantable or prime tubers, while those on the right are the culls. SEED-SELECTION PLAT. The accompanying diagram (fig. 2) of a plant- ing plan is presented more as a suggestion for use by boys' and girls' potato clubs than for the average potato grower. A hundred tubers make a convenient unit to handle, which at the same time is capable of unlimited expansion in the hands of those whose enthusiasm makes them willing to undertake larger operations. Each tuber unit in the diagram is numbered in order to facilitate note taking, as well as the keeping of a record on the behavior of each individual tuber unit throughout the season. By affixing their numbers to the units selected for another season's trial it is possible to pre- serve the identity of each selection throughout whatever period of years it may be studied. [Cir. 113) \h§ ^O ^-0 0)0 (VtO SfrO NO tfvO '0 '0 /v.O ~.o _0 (*)0 H>0 ^O 8 /^O ,0 O i_0 £>o 90 00 *>o (\jo TSO £0 vTvO "0^0 ' '0 ~ ° W.O «. ° n. ° o «0o t*5o \0 V>0 v©o 0\O '0 « . O f.,0 NO C\lo No ^O ^o sso voo 0)0 ,00 .„o voo ^-0 ^0 ^0 \O\hOV0OJ)O -. loo Oo 'oo Oo o °>0 (V\0 1X0 V^jO J o '0 ->o ^0 (r>0 ^O QJO Do <0g p,o Eg Qdo . §cvj8n§j\8 No (nO InO VTQO O 'O 'O O O O.O O (p,o "^0 V)o \o ^O OrvO l(\0 ttJO '0 'o O rsO 1 _ O inO Oo >oo Oo ^0 Ooo loo vxo J o n\° «° mO *lo J)o S-o Jio ^0 (\jo >oo No oooo C*8$8fc8$o8 ^0 CVjo "oo No oooo k° ^ „ ^0 CVjo loo No 000 1-.0 O lr.O Cvjo 'OO N OOOO a a 5 I oi 6 £ "TUBER-UNIT" METHOD OF SEED-POTATO IMPROVEMENT. 29 The seed-selection plat need not be divorced from the potato field. It can quite as readily be a portion of it, provided it is so located as to make it easily accessible for note taking. NOTE BLANKS. The note blanks shown in Table 1 are those which have been prepared for use in the Office of Horticultural Investigations and Arlington Farm. Note Blank I is prepared with especial reference to the first season's work. Note Blank II is designed for notes to be taken on the selected tuber units hi the second and subsequent season in which they are- studied. The only difference between the two blanks is with respect to the notes on the upper part of the sheet in which provision has been made in Note Blank II for recording the previous year's data relative to the time and place of selection of the tuber unit, together with yield of tubers secured. With such data recorded, the observer can very readily compare the behavior of the selection in any given season with that of the preceding one. Each note b'ank has provision for notes on 10 tuber units. SUMMARY. (1) Good seed is of vital importance in the production of a maxi- mum crop of potatoes. (2) Good seed may be obtained by the tuber-unit method through the elimination of unproductive and weak plants. (3) Like produces like. Plant tubers from unproductive or weak plants and you will reap a similar harvest. [Cir. 113] 30 CIRCULAR NO. 113, BUREAU OF PLANT INDUSTRY, O s 5. £ w pq < c P u m u 03 W a o o (* u* t*-. BS a © o fc. 60 O pq w H o 4 £ 3 - I o a. T3 C3 4> e o > o a o 4> 5 a 3 « 'r St; 41 o >.£. en J- i Total weight. . o Ml °3 N V Num- ber small. ! 2 to ■/'. -; £ Q •« Li -.2.5 • •-7c a a; ~ CO a a) 4) 22 so O 41 Num- ber plants. ■r E Date sprouts appear. 31 SOll Weight of tuber (ounces). S o O 41 ■ .a o c z C3 X! O 7 fc J; a a a rt H a p. >. o .tj 4 2 4 c 41 41 — .5 •- -C O 4 3 S O 0J s a ft -a o . 4 C S fi •c 3 itf^ ■a e — ' 03 4i S * 0J «s (H - > a, .. a a a 4> 4) S o 2 i S T3 i" •3 £ rt o ■» > ** T) l — X) O o 4) o g 6£ s •r I* o 05 4> e -3 -SP a CO r$ ;- 4> -4^» EH + a a • *. •rt ■g 1 5 2 nf t-i o — > -a R ^ C3 4 © o V. a. 4> 5JCD o » 3 ^ « s a -i 60 • a a 41 2.2 ^ § S 41 a. 41 3 E " 4 S ?7 [Cir. 113] "TUBER-UNIT" METHOD OF SEED-POTATO IMPROVEMENT. 31 a : H y. u & a w w H CO - a s. c o H X K a u E; c o O C6 3 a ■a 3 3 o & it s o p. 6C ■a 03 O a. - r- > .C o 3 X! ,0 pH -a ■d I c o 5 3 p *> a) cj o 75 70 75 < /. M CO Unit saved or rejected. : +3 ^1 O to 6f to" Num- ber small. 60 1^* ;- QJ . — I a bo a u. - 1 ■r. « 3 H a 1 ! O 03 s'S 1 -— C3 CO ■*-> c n Desirable, Un- desirable. $4 3 c6 svri =5 9 * Num- ber plants. 1 3 o i- p. • ■ Date sprouts appear. Weight of tuber ( ounces 1. 5 . ^ 6 ! & > & ^ — ■ f o o [Cir. 113] U. S. DEPARTMENT OF AGRICULTURE. BUREAU OF PLANT INDUSTRY— Circular No. 114. B. T. GALLOWAY, Chief of Bureau. MISCELLANEOUS PAPERS. Sowing Flax on Winterkilled Wheat Fields Experiments in Subsoiling at San Antonio M. W. EVANS Control of the Black-Rot and Stem-Rot of the Sweet Potato Bartlett Pear Precooling and Storage Investigations in the Rogue River Valley Climatic Conditions on the Truckee-Carson Project . I S. H. HASTINGS 1 and C. R. LETTEER L. L. HARTER A. V. STUBENRAUCH and H.J. RAMSEY . F. B. HEADLEY Issued February 24, 1913. WASHINGTON: GOVERNMENT PRINTING OFFICE. 1913. BUREAU OF PLANT INDUSTRY. Chief of Bureau, Beverly T. Galloway. Assistant Chief of Bureau, William A. Taylor, Editor, J. E. Rockwell. Chief Clerk, James E. Jones. [Cir. 114] 9 SOWING FLAX ON WINTERKILLED WHEAT FIELDS. 1 By M. W. Evans, Scientific Assistant, Forage-Crop Investigations. INTRODUCTION. Wherever wheat is sown in the fall and the climatic conditions during the winter are unfavorable, it is more or less subject to injury by winterkilling. Great losses are sometimes sustained in the winter- wheat areas of the United States from this cause. The farmers in the vicinity of New London, in northern Ohio, have found a way to avoid much of this loss. AVhen the stand of Pig. 1. — A Hold of fall-sown wheat and spring-sown flax at New London, Ohio. wheat in the spring is very thin, flax is frequently sown on the field. In the spring of 1912, when the wheat fields were in very bad condi- tion as a result of winterkilling, a large proportion of the wheat acreage in that locality was seeded with flax. In this vicinity a 5-year rotation, consisting of corn, oats, wheat, timothy and clover, and timothy, is generally followed. Occasionallv [Clr, 114] 1 Issued Feb. 22, 1913. 4 CIRCULAR NO. 114, BUREAU OF PLANT INDUSTRY. flax is sown instead of oats. Sometimes the meadow is plowed up after one year and sometimes it produces hay for three years or more, but the rotation just described is the customary one. How or when the practice of sowing flax in wheat originated has not been definitely determined. It has been followed around New Lon- don, however, for a period of approximately twenty years. Although careful inquiry has been made, no information has been obtained that flax is sown on winter-wheat fields in any other locality in the United States. There is apparently no reason why the practice would not be applicable in other parts of the country where flax thrives and where wheat is subject to winterkilling. CONDITIONS UNDER WHICH FLAX IS SOWN. Ordinarily when wheat has been injured by winterkilling, the farmer either harvests a light crop that does not pay for the cost of producing it or else he prepares the soil for a crop of spring grain, which not only involves additional expense, but also often seriously interferes with his regular rotation of crops. It is under such condi- tions that the practice of sowing flax in the wheat field has been found to be a means of avoiding loss and interference with the regu- lar system of crop rotation. It is a common practice of the farmers in the vicinity of New London to sow timothy seed at the same time as the wheat. The weather conditions in the fall of 15)11 were very favorable for the growth of the timothy seedlings. In the spring of 1912 there was such a well-developed stand of timothy in some of the wheat fields that it was evident that the fields would produce a good crop of mixed wheat and timothy hay or, if left to mature, a light crop of wheat and a fair crop of timothy seed. Flax is not sown, as a rule, in fields where the indications are that the timothy will produce a fair crop. SEEDING. The flax is generally sown about April 1, or as soon as the weather has become warm enough to be favorable for plant growth and dan- ger of freezing weather is past. Light frosts that sometimes occur after the flax seedlings are up do not seem to harm them. The seed is sown as early as it is safe to do so; otherwise, the flax does not mature until somewhat later than the wheat. When the flax is sown as early as April 1 it matures in time, so that there is practically no loss of wheat through shattering, as is the case when it is sown after the middle of April. The flax is ordinarily sown broadcast with one of the various types of hand seeders. A few farmers mix the flax with timothy and clover and sow them together. As a rule the flax is sown separately and sometimes at a later date than the clover. [Cir. 114] SOWING FLAX ON WINTERKILLED WHEAT FIELDS. 5 Flax germinates very readily. The farmers sow the seed without harrowing- the soil either before or after sowing. When the first rain occurs, the seed germinates and usually makes a good stand. A light harrowing, however, would no doubt be beneficial to both the flax and the wheat. When a flax crop is grown alone, the farmers in this vicinity gen- erally sow 1 bushel of seed to the acre. When the flax seed is sown on winter-wheat fields, different farmers sow amounts of seed varying from '2 to 4 pecks per acre. The quantity of seed sown also depends somewhat upon the extent to which the wheat has been winterkilled. HARVESTING. As the wheat ripens somewhat earlier than the flax, the crop is harvested as soon as possible after the flax is mature. As has already been stated, the date at which the flax is ready to harvest depends largely upon the date on which the seed was sown. As flax matures, its stems become woody, so that it is compara- tively difficult to cut. The knives of the machine used must therefore be sharp. Flax is harvested in various ways. Farmers who own a drop-rake reaper usually use that machine. Where there is a large proportion of wheat growing in all parts of the field in mixture with the flax, a binder does excellent work. In fields where the flax pre- dominates, the binder does not work so well. Where the flax grows alone, particularly if it is short and fine, it tends to get under the canvas and become wound around different parts of the machine, so that it sometimes becomes necessary to stop the machine and remove the flax. Most of the flax grown in this locality is cut with a mowing machine, with an attachment behind the cutting bar which leaves it in light windrows, out of the way of the horses' feet and the wheels of the mower when the next swath is cut. By means of a 1-horse wheel rake, two windrows may be gathered at once into small bunches, where the flax lies until dry. The flax may be loaded, pref- erably with large wooden forks, on hayracks and hauled directly to the thrashing machine or stored in a barn or stack until it can be thrashed. THRASHING AND SEPARATING THE SEED. The wheat and flax mixture is thrashed in the same way that wheat alone is thrashed. They go through the same sieves and come out through the grain spouts together. The two kinds of seed are later separated with a fanning mill. The work of separating is usually done at the grain warehouse by the dealer who purchases the seed. [Cir. 114] 6 CIRCULAR NO. 114, BUREAU OF PLANT INDUSTRY. When a mixture of flax, wheat, and timothy is thrashed the timo- thy seed is separated by the sieves from the flax and the wheat and conies out beneath the machine. The cost of thrashing is determined by the proportion of flax and wheat after the seed has been separated. In this locality the thrashers charge 12^ cents per bushel for thrashing flax and 4 cents per bushel for wheat. YIELDS. The yield of flax and wheat depends upon several factors. Soil and climatic conditions, as well as the relative thickness of the stand of flax and wheat, are important. In Table I are given the yields harvested from several typical fields in 1912. It should be understood that neither the area of the fields nor the yields were measured exactly: the figures are quoted from the statements of the farmers growing the crop and are no doubt approximately correct. Table I. — Yields of flax, wheat, and timothy bushels of wheat to the acre. UTILIZATION OF SEED AND STRAW. The flax seed is usually sold to a local grain dealer. It is then shipped to mills which manufacture linseed oil and linseed meal. Large quantities of thrashed flax straw produced on fields where the flax has been grown alone are sold at a local tow mill. When the flax is grown in mixture with wheat, however, it can not be used for making tow unless it contains only a very small proportion of the wheat straw. The price of clear flax straw is at present $8 per ton. Flax straw containing a small amount of wheat or timothy was sold from a few farms for $7 per ton. As a rule, the mixed flax and wheat straw is retained on the farm, to be used for bedding or to be i'vd in the barnyard to stock during the winter. EFFECT OF THE FLAX ON THE WHEAT, TIMOTHY, AND CLOVER. It is the general opinion of those farmers who have sown flax in their wheat fields that the amount of wheat produced is not appre- ciably decreased by the presence of the flax. In those spots in the field where there may be a good stand of wheat plants the flax seed- lings make only a small growth. In those portions of the field where the wheat has been winterkilled w T eeds would grow if the flax were not present. Timothy and clover are sown in a field where flax is growing in mixture with the wheat in the same way that they are sown with wheat growing alone. Many good meadows have been obtained where timothy and clover have been seeded with wheat and flax. CONCLUSION. This method of sowing flax in wheat fields is not recommended as a regular farm practice. It is merely a plan that may be resorted to in years when wheat has been injured by winterkilling. After being given a trial of 15 or 20 years in the locality where the method was originated it is looked upon as one of the best ways to make use of a wheat field that has been injured by winterkilling. There is appar- ently no reason why it could not be adapted to a large proportion of the winter-wheat area throughout the Eastern and Central States, where it could be made a means of decreasing the loss that frequently occurs in seasons following a winter unfavorable to the wheat crop. [Clr. 114] EXPERIMENTS IN SUBSOILING AT SAN ANTONIO. 1 P.y S. II. Hastings, Farm Superintendent, and C. u. Letteer, Assistant, Office of Western Irrigation Agriculture. INTRODUCTION. San Antonio, Tex., is in what is classified as a semiarid region, the normal annual rainfall, as shown by the records of the United States Weather Bureau for the years 1891 to 1910, inclusive, being 24.77 inches. Because of the irregularity of the rainfall, the com- pact character of the soil, which during torrential rainstorms causes a large loss in surface run-off, and the high evaporation as compared with more northern sections, soil moisture is one of the most im- portant limiting factors in crop production. Any farm practice, therefore, which will result in increased moisture content of the soil will be of much importance to the agriculture of the region. It is popularly thought that subsoiling will effect this and by so doing will increase crop production. Subsoiling consists in loosening the soil to a depth greater than it can be loosened with an ordinary plow. This is accomplished by a subsoil plow, which operates in the bottom of the furrow left by a breaking plow, loosening the soil to the additional depth of about 12 inches. It requires a 2-horse or 3-horse team and a man to per- form the operation, in addition to the man and team that do the plowing, so that the cost of plowing and subsoiling is practically double what is required for ordinary breaking. It is readily seen that the practice is an expensive one, and adds so much to the cost of preparation for a crop that unless it results in materially increased yields it can not be profitably followed as a regular farm practice. The cost of subsoiling at San Antonio has been found to be practi- cally the same as plowing, or about $2 per acre. SOIL CONDITIONS OF THE REGION. 2 San Antonio lies in the southern extension of what is known as the Black Prairie region, or the Black Lands of Texas, and near the northern edge of an area known geologically as the Rio Grande i Issued Feb. 22, 1913. "See Circular 34, Bureau of riant Industry, U. S. Department of Agriculture, entitled " The work of the San Antonio Experiment Farm in 1908." 78241°— Cir. 114—13 2 9 10 CIRCULAR NO. 114, BUREAU OF PLANT INDUSTRY. Plain. The soil .is mostly the result of weathering of limestone rocks of the Upper Cretaceous period. Recent alluvial deposits have. been washed down from the higher lands northwest of the city, re- sulting in local modifications through the addition of coarser mate- rial. The typical soil is a heavy black or brownish clay or clay loam. The lime content of the soil is unusually high, the proportion of lime in the upper 12 inches of soil varying from 7 to 23 per cent, This lime occurs in the soil both as finely divided material and as gravelly concretions. In the former condition it is generally dark colored through staining by decomposed organic matter, while in the latter condition it is usually white. TEST OF SUBSOILING IN ROTATION AND TILLAGE EXPERIMENTS. In order to determine the effect of subsoiling upon crop yields and upon the moisture content of the soil at San Antonio, subsoiling tests as a part of the rotation and tillage experiments were begun on the San Antonio Experiment Farm in" the fall of 1009. This rota- tion and tillage work is conducted on 82 plats, each one-fourth acre in size. Three years' results have been obtained with subsoiling, and it is believed that these results are sufficiently conclusive to justify their publication. Corn, cotton, oats, grain sorghums, and sac- charine sorghums are the crops grown in the rotations. The va- rieties of the various crops referred to in this paper are as follows: Corn (Laguna), three years; oats (Appier's Tviistproof), three years; cotton (Triumph)'. 1010 and 1011, (Acala). 1012. Those rotations in which subsoiling has been tested are shown in Table I. The lists are so arranged that the rotations opposite each other are directly comparable, the only difference in treatment being that of subsoiling or its omission. Table I. Lists of rotations in which subsoiling has been tested at San Antonio Experiment Farm. Yr. Not subsoiled. Rotation A5-D: i late for grain, cowpeas; plow under cowpeas in fall. Cotton; plow in November. Com, cowpeas, manure; plow under cowpeas in fall. Cotton; plow in November. Rotation A 6- A: Corn, plow in July. Oats for hay; plow in May. Rotation 15«V A: Corn: plow in July. Cotton; plow in November, isolation B6-D: Corn, cowpeas, manure: plow cowpeas in fall. Cotton; plow in November. Rotation B6-I i : Corn: plow in February. Cotton; plow in February. Subsoiled. Rotation A5-E: ' >ats for grain, cowpeas; plow and subsoil in fall. Cotton; plow in November. Com, cowpeas, manure; plow cowpeas and subsoil in fall. Cotton; plow in November. Rotation A6-C: Corn; plow in July. < »ats for hay; plow and subsoil in May. Rotation B6-B': Corn: plow and subsoil in July. Coll on: plow and subsoil in November. notation B6-E: Corn, cowpeas, manure; plow cowpeas in fall. Cotton; plow and subsoil in November. Rotation lie. II: Com; plow and subsoil in February. Cotton; plow and subsoil in February. [Cir. 114] EXPERIMENTS IX SUBSOILING AT SAX ANTONIO. 11 EFFECT OF SUBSOILING ON CROP YIELDS. Tabic II shows the effects of subsoiling on the yields of corn, cot- ton, and oats. Table II. — Average yield of crops in rotations subsoiled and rotations not sub- soiled at the San Antonio Experiment Farm, WW to 191.2, inclusive. Rotation Nos. Not sub- soiled. Sub- soiled. (iain( + ) or loss ( — ). Rotation Nos. Not sub- soiled. Sub- soiled. Gain( + ) or loss ( — ). COKN (Bushels teh Acre i. A6-A and A6-C B(V-A and B6-B.... B6-Gand B6-H.... 13.0 24. S 21.1 21.2 20.9 1 1.0 ■rl 2 Is! 7 20.6 17.9 + 1.0 —2.6 -2.4 - .0 -3.0 Cotton ( Pounds of Ski: n Cotton per Acre). A.5 I) and A5-E B6 A and B0-B B6-G and B6-H Average Average of 10 rota- tions: 1910 4S7.3 5119.3 501.7 614.0 574.7 436.7 520.0 5iii.:i 643.3 502.0 - 50.0 + 10.7 - 00.4 + 29.3 - 72.7 Average 20.2 18.7 -1.5 549.4 521.9 - 27.5 Average of LO rota- tions: 1910 10.8 11.9 37.9 7.2 11.8 37.0 -3.6 - .1 - .9 420.0 507.2 714.4 397.0 190. 1 077.0 - 29.0 I'll i 1911 - 16.8 1912 1912 - 30.8 3-year average. Oats (Pounds of Hay per Acre). A0- A and A6-C: 3-ycar average... Average of 2 rota- tions: 1910 3-year average. 20.2 IS. 7 -1.5 549. 4 521.9 - 27.5 Oats (Bushels per Acre). A5-I) and A5-E: 3-year average. . Average of 2 rota- tions: 1910. . 11.5 5.1 5.4 24.1 11.7 3.8 0. 2 25.0 + .2 -1.3 + .8 + .9 3,557 2,050 3,056 5,560 3,121 2,344 1,9111 5,056 - 430 + 2SS 1911 1911 -1,092 1912.. 1912 - 504 Summary of Averages. Crop. Corn bushels. . Cotton pounds.. Oats, grain bushels. . Oats, hay pounds. . A verage Yield per acre. Not sub- soiled. 20.2 549. 4 11.5 3,557 Sub- soiled. 18.7 521.9 11.7 3,121 Gain(+) or loss (— ). - 1.5 - 27.5 + .2 -430 Yield expressed in percent- ages of untreated plats. Not sub- soiled. 100 100 100 100 100 Sub- soiled. 92.6 95 101.7 87.7 94. 25 Gain(-f) or loss (— ). -7.4 - 5 + 1-7 -12.3 From the foregoing table it is seen that in four rotations snl (soil- ing has decreased the yields of corn and in one rotation it has slightly increased the yield. The cotton yields have been increased in two rotations and decreased in three rotations. In those portions of the table showing the average yield by years it is seen that subsoil- ing gave decreased average }delds of corn and cotton in each of the three years. The summary shows that when the three-year average for all crops concerned is taken into consideration, subsoiling has decreased the yield of all crops except oats for grain. Since the tests with oats have not been so extensive as with other crops, it is doubt- ful whether this result can be taken as conclusive. [Cir. 114] 12 CIRCULAR NO. 114, BUREAU OF PLANT INDUSTRY. RESIDUAL EFFECT OF SUBSOILING ON THE YIELDS OF CORN AND COTTON. In order to call attention to the possible residual effect of subsoil- ing on succeeding crops for several years after the operation, Table III has been compiled to show the effect of subsoiling on the yields of corn and cotton where the length of time intervening between the time of subsoiling and that of planting varies from 1 to 16 months. Table III.— Effect of different lengths of time between subsoiling and planting on the average yield of corn and vOlton at the Han Antonio Experiment Fa rm. Crop. Cain or loss, per acre, due to sub- soiling. 15 lo lti months. 3toS months. 1 to 2 months. bushels. . + 1.3 + 7 - 1.5 -31.4 - 3.1 pounds. . -72.7 There is a marked decrease in the yields of both corn and cotton where the subsoiling was done from 1 to 2 months before planting, and a decrease where the subsoiling was done from 3 to 8 months before planting, while when the subsoiling was done from 15 to 10 months before planting there was a very slight increase. While these results are not at all conclusive, owing to the fact that the experiments cover only two years and the number of plats is limited, yet they are suggestive. The figures in this table indicate that loosen- ing the subsoil is detrimental the first few months and also at the end of over a year, when under ordinary conditions if there was to be any resulting benefit it should be apparent. During the interven- ing period between subsoiling and planting, a crop was grown and the land replowed, and it would be supposed that any unfavorable soil conditions brought about by subsoiling would have time to read- just themselves during the period. Yet the increase in average yields as shown by the table is so slight as to indicate very little result- ing benefit from the operation. In no case was the increase sufficient to cover the expense of subsoiling. EFFECT OF SUBSOILING ON THE MOISTURE CONTENT OF THE SOIL. Determinations of soil moisture were made at regular intervals of about one month on rotations B6-A and BG-B for a period of two years. Table IV shows the percentage of moisture in plat B6-1 as compared with B6-3, and BG-2 as compared with B6-4; also the crops grown on each plat each year and the time of plowing or subsoiling. The soil samples for moisture determinations were taken by means of the standard soil tube, two cores being taken on each plat and composited to a single sample. This composite sample was then weighed, dried, and weighed again, and the percentage of moisture computed on the basis of dry soil. [C'ir. 114] EXPERIMENTS IN SUE-SOILING AT SAN ANTONIO. 13 Table IV. — Average moisture content in land subsoiled and land not subsoiled at the San Antonio Experiment Farm.' Crop and year. Plat B6-1. Plat B 6-3. Sampled. Plowed. Moisture content. Plowed and subsoiled. Moisture content. 1910. Nov. 21 November do 10.9 1.3.3 12.4 11.9 12.4 13.5 14.1 8.7 9. 5 9.8 10.1 17.3* 16.2* 14.0 16.6* 16.6 20.2* 17.9* 16. 13.5* 10.5 10.6* 14.7* November do do do do do do do do 1911. do do do do do do do do do do do do do 11.7 Dec. 27 11.6 1911. Jan. 12 do 11.0 Feb. 13 do 11.7 Mar. 11 Corn, 1911 do 10.3 do do 12.4 Ma v 9 ..do ..do 13.7 June 15 ..do do 8.9 July 17 ..do ..do 9.1 Aug. 17 ..do 1911. do 9.5 Sept. 16 do 9.4 Nov. 16 ..do .do.... is. 3* Dec. 13 ..do do 16. 7* 1912. Jan. 9 do... .do . 12.0 Feb. 9 ...do ...do 16.4* Mar. 11 ..do ..do 16. 2 Apr. 17 Cotton, 1912 do do L9.6* 17. S* June 12 ..do ..do... 14. S July 9 ..do... .do... 12.6* Aug. 22 do ...do 9.2 do do Hi :;i : Oct. 24 ..do... .do... 14.8* Mean 13.0 12.4 Crop and year. Plat B 6-2. Plat 15 6-4. Sampled. Plowed. Moisture content. Plowed and subsoiled. Moisture content. 1910. Aug. 17-18 Corn, 1910 do Aug. 1 . . . . do 10.2 9.1 11.0 11.3- 11.7 11.1 11.5 10.8 12.5 13.7 10.5 9.7 lu.1 9.4 16.6* 15.0* 11.5 16.9* 15.3 20.5* 17.1* 11.5 1 1 . 3* 9.8 10.9* 15.2* Aug. 2.... do do do do do do do do do do do do do do.... 1911. Nov. 29... do do.... do do do do do do do do 10.8 Sept. 19 9.2 Oct. 14 ..do... ....do 10.5 Nov. 21 ..do... ..do... 9.6 Dec. 27 ..do... do... 11.9 1911. Jan. 12 ..do do 10.0 do 11 6 Mar. 11 .do... ..do... 10.6 Cotton, 1911 do.. do .do.. 13 May 9 13.7 June 1") ...do ..do 8.6 July 17 .do .do... 9.3 Aug. 17 .do... .do... 8.3 Sept. 16 .do... .do . S. 3 Nov. 16 .do... ..do... 1.")..")* 1911. Dec. 11.... . do 16. 2* 1912. Jan. 11 do .. 12.2 Feb. 9 do .do 15. 2* Mar. 11 Corn, 1912. . .do .. 16.7 Apr. 17 .do... do 18.9* May 18 .do... do . 16.7* June 12 .do... ..do... 1 2. 6 July 9 .do... ..do... 10.8* Aug. 22 .do .do... 9.9 do 9.2* Oct. 24 .do ..do... 15.1* Mean 11.9 11.6 1 Each moisture determination marked with a star (*) is an average of 3 feet; all others are averages of 6 feet. rCir. 114] 14 CIRCULAR NO. 114, BUREAU OF PLANT INDUSTRY. Table IV shows that in only a very few instances has the percent- age of moisture in subsoiled land been greater than that in land not subsoiled, while in most instances it has been somewhat less. The means were obtained, not from the monthly averages, but by con- sidering the entire number of samples taken during the sampling period. In both pairs of plats the averages indicate a larger per- centage of soil moisture in land not subsoiled than in the subsoiled land. CONCLUSIONS. (1) Subsoiling is an expensive practice and so adds to the cost of preparation for a crop that unless materially increased yields result it can not be profitably adopted as a regular farm practice. (2) Subsoiling has been tested at the San Antonio Experiment Farm for three years in rotation experiments with corn, cotton, and oats for hay and for grain. (3) The yields of corn, cotton, and oats for hay and for grain have been either slightly increased or slightly decreased on subsoiled land. In no instance has the difference been significant. (4) The depressing residual effect of subsoiling on the yields of corn and cotton was most marked when the crop was planted from 1 to 8 months after subsoiling; 15 months after subsoiling but little depressing effect was noted. (5) In the soil-moisture studies so far made at San Antonio it has been found that subsoiling has not increased the moisture content of the soil. ((>) The results of these tests indicate that since neither the moisture content of the soil nor the yields of corn, cotton, and oats are increased by subsoiling, the practice is not advisable in connec- tion with the crops mentioned in the San Antonio region of Texas. [Cir. 114] CONTROL OF THE BLACK-ROT AND STEM-ROT OF THE SWEET POTATO. 1 By L. I.. Harter, Pathologist, Cotton and Truck Disease and Sugar-Plant Investigations. INTRODUCTION. This article outlines some of the precautions necessary to reduce the widespread loss of the sweet-potato crop by the black-rot 2 and stem-rot 3 organisms. Several papers on sweet-pot